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Protein

3-deoxy-manno-octulosonate cytidylyltransferase

Gene

kdsB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.By similarity

Catalytic activityi

CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate.

Pathwayi: CMP-3-deoxy-D-manno-octulosonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP.
Proteins known to be involved in this subpathway in this organism are:
  1. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB)
This subpathway is part of the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP, the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BRENDAi2.7.7.38. 2529.
UniPathwayiUPA00030.
UPA00358; UER00476.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase (EC:2.7.7.38)
Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name:
CKS
Short name:
CMP-KDO synthase
Gene namesi
Name:kdsB
Ordered Locus Names:HI_0058
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 2542533-deoxy-manno-octulosonate cytidylyltransferasePRO_0000188506Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0058.

Structurei

Secondary structure

1
254
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85Combined sources
Beta strandi14 – 163Combined sources
Helixi19 – 213Combined sources
Beta strandi25 – 273Combined sources
Helixi29 – 3911Combined sources
Beta strandi43 – 508Combined sources
Helixi52 – 609Combined sources
Beta strandi64 – 674Combined sources
Helixi75 – 8511Combined sources
Beta strandi93 – 964Combined sources
Helixi106 – 11914Combined sources
Beta strandi122 – 1298Combined sources
Helixi133 – 1364Combined sources
Beta strandi143 – 1464Combined sources
Beta strandi150 – 1589Combined sources
Helixi164 – 1674Combined sources
Helixi173 – 1753Combined sources
Beta strandi183 – 19210Combined sources
Helixi193 – 2019Combined sources
Helixi206 – 2116Combined sources
Helixi216 – 2205Combined sources
Beta strandi225 – 2295Combined sources
Helixi241 – 25313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VH3X-ray2.70A/B/C2-254[»]
1VICX-ray1.80A/B2-254[»]
3DUVX-ray2.30A/B1-254[»]
ProteinModelPortaliP44490.
SMRiP44490. Positions 2-254.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44490.

Family & Domainsi

Sequence similaritiesi

Belongs to the KdsB family.Curated

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
KOiK00979.
OMAiNSGTERC.
PhylomeDBiP44490.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. CMP_KDO_synth. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTVIIPAR FASSRLPGKP LADIKGKPMI QHVFEKALQS GASRVIIATD
60 70 80 90 100
NENVADVAKS FGAEVCMTSV NHNSGTERLA EVVEKLAIPD NEIIVNIQGD
110 120 130 140 150
EPLIPPVIVR QVADNLAKFN VNMASLAVKI HDAEELFNPN AVKVLTDKDG
160 170 180 190 200
YVLYFSRSVI PYDRDQFMNL QDVQKVQLSD AYLRHIGIYA YRAGFIKQYV
210 220 230 240 250
QWAPTQLENL EKLEQLRVLY NGERIHVELA KEVPAVGVDT AEDLEKVRAI

LAAN
Length:254
Mass (Da):28,255
Last modified:January 23, 2007 - v2
Checksum:i2FAC2BB15BD961A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21736.1.
PIRiG64045.
RefSeqiNP_438231.1. NC_000907.1.
WP_005693864.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21736; AAC21736; HI_0058.
GeneIDi950957.
KEGGihin:HI0058.
PATRICi20188569. VBIHaeInf48452_0058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21736.1.
PIRiG64045.
RefSeqiNP_438231.1. NC_000907.1.
WP_005693864.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VH3X-ray2.70A/B/C2-254[»]
1VICX-ray1.80A/B2-254[»]
3DUVX-ray2.30A/B1-254[»]
ProteinModelPortaliP44490.
SMRiP44490. Positions 2-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21736; AAC21736; HI_0058.
GeneIDi950957.
KEGGihin:HI0058.
PATRICi20188569. VBIHaeInf48452_0058.

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
KOiK00979.
OMAiNSGTERC.
PhylomeDBiP44490.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00358; UER00476.
BRENDAi2.7.7.38. 2529.

Miscellaneous databases

EvolutionaryTraceiP44490.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. CMP_KDO_synth. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKDSB_HAEIN
AccessioniPrimary (citable) accession number: P44490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.