Reviewed, UniProtKB/Swiss-Prot P44490 (KDSB_HAEIN)

Names and origin

Protein names

Recommended name:
    3-deoxy-manno-octulosonate cytidylyltransferase
    EC=2.7.7.38
Alternative name(s):
    CMP-KDO synthetase
    CMP-2-keto-3-deoxyoctulosonic acid synthetase
      Short name=CKS

Gene names

Name:	kdsB
Ordered Locus Names:	HI0058

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity.
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the kdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 254

253

3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057

PRO_0000188506

Secondary structure

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254

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Beta strand

14 – 16

3

Helix

19 – 21

3

Helix

29 – 39

11

Beta strand

43 – 50

8

Helix

52 – 60

9

Beta strand

64 – 67

4

Helix

75 – 85

11

Beta strand

93 – 96

4

Helix

106 – 119

14

Beta strand

122 – 129

8

Helix

133 – 136

4

Beta strand

143 – 146

4

Beta strand

150 – 158

9

Helix

164 – 167

4

Helix

173 – 175

3

Beta strand

183 – 192

10

Helix

193 – 201

9

Helix

206 – 211

6

Helix

216 – 220

5

Beta strand

225 – 229

5

Helix

241 – 253

13

Sequences

	Sequence		Length	Mass (Da)
	P44490-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2FAC2BB15BD961A2	FASTA	254	28,255
	10 20 30 40 50 60 MSFTVIIPAR FASSRLPGKP LADIKGKPMI QHVFEKALQS GASRVIIATD NENVADVAKS 70 80 90 100 110 120 FGAEVCMTSV NHNSGTERLA EVVEKLAIPD NEIIVNIQGD EPLIPPVIVR QVADNLAKFN 130 140 150 160 170 180 VNMASLAVKI HDAEELFNPN AVKVLTDKDG YVLYFSRSVI PYDRDQFMNL QDVQKVQLSD 190 200 210 220 230 240 AYLRHIGIYA YRAGFIKQYV QWAPTQLENL EKLEQLRVLY NGERIHVELA KEVPAVGVDT 250 AEDLEKVRAI LAAN

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L42023 Genomic DNA. Translation: AAC21736.1.

PIR

G64045.

RefSeq

NP_438231.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VH3	X-ray	2.70	A/B/C	2-253	[»]
1VIC	X-ray	1.80	A/B	2-253	[»]
3DUV	X-ray	2.30	A/B	1-254	[»]

ModBase

Search...

Genome annotation databases

GeneID

950957.

GenomeReviews

Gene locus HI0058 in contig L42023_GR.

KEGG

hin:HI0058.

NMPDR

fig|71421.1.peg.57.

TIGR

HI0058.

Phylogenomic databases

HOGENOM

P44490.

OMA

FSRAPLP

Enzyme and pathway databases

BioCyc

HINF71421:HI_0058-MON.

BRENDA

2.7.7.38. 109.

Family and domain databases

HAMAP

MF_00057.
[Tree]

InterPro

IPR003329. Cytidylyl_trans.
IPR004528. KdsB.
[Graphical view]

Pfam

PF02348. CTP_transf_3. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00466. kdsB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

KDSB_HAEIN

Accession

Primary (citable) accession number: P44490

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)