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Protein

3-deoxy-manno-octulosonate cytidylyltransferase

Gene

kdsB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.UniRule annotation

Catalytic activityi

CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate.UniRule annotation

Pathwayi: CMP-3-deoxy-D-manno-octulosonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB)
This subpathway is part of the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP, the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BRENDAi2.7.7.38. 2529.
UniPathwayiUPA00030.
UPA00358; UER00476.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferaseUniRule annotation (EC:2.7.7.38UniRule annotation)
Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthaseUniRule annotation
Short name:
CKSUniRule annotation
Short name:
CMP-KDO synthaseUniRule annotation
Gene namesi
Name:kdsBUniRule annotation
Ordered Locus Names:HI_0058
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001885062 – 2543-deoxy-manno-octulosonate cytidylyltransferaseAdd BLAST253

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0058.

Structurei

Secondary structure

1254
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi14 – 16Combined sources3
Helixi19 – 21Combined sources3
Beta strandi25 – 27Combined sources3
Helixi29 – 39Combined sources11
Beta strandi43 – 50Combined sources8
Helixi52 – 60Combined sources9
Beta strandi64 – 67Combined sources4
Helixi75 – 85Combined sources11
Beta strandi93 – 96Combined sources4
Helixi106 – 119Combined sources14
Beta strandi122 – 129Combined sources8
Helixi133 – 136Combined sources4
Beta strandi143 – 146Combined sources4
Beta strandi150 – 158Combined sources9
Helixi164 – 167Combined sources4
Helixi173 – 175Combined sources3
Beta strandi183 – 192Combined sources10
Helixi193 – 201Combined sources9
Helixi206 – 211Combined sources6
Helixi216 – 220Combined sources5
Beta strandi225 – 229Combined sources5
Helixi241 – 253Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VH3X-ray2.70A/B/C2-254[»]
1VICX-ray1.80A/B2-254[»]
3DUVX-ray2.30A/B1-254[»]
ProteinModelPortaliP44490.
SMRiP44490.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44490.

Family & Domainsi

Sequence similaritiesi

Belongs to the KdsB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
KOiK00979.
OMAiNSGTERC.
PhylomeDBiP44490.

Family and domain databases

CDDicd02517. CMP-KDO-Synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. KdsB. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTVIIPAR FASSRLPGKP LADIKGKPMI QHVFEKALQS GASRVIIATD
60 70 80 90 100
NENVADVAKS FGAEVCMTSV NHNSGTERLA EVVEKLAIPD NEIIVNIQGD
110 120 130 140 150
EPLIPPVIVR QVADNLAKFN VNMASLAVKI HDAEELFNPN AVKVLTDKDG
160 170 180 190 200
YVLYFSRSVI PYDRDQFMNL QDVQKVQLSD AYLRHIGIYA YRAGFIKQYV
210 220 230 240 250
QWAPTQLENL EKLEQLRVLY NGERIHVELA KEVPAVGVDT AEDLEKVRAI

LAAN
Length:254
Mass (Da):28,255
Last modified:January 23, 2007 - v2
Checksum:i2FAC2BB15BD961A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21736.1.
PIRiG64045.
RefSeqiNP_438231.1. NC_000907.1.
WP_005693864.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21736; AAC21736; HI_0058.
GeneIDi950957.
KEGGihin:HI0058.
PATRICi20188569. VBIHaeInf48452_0058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21736.1.
PIRiG64045.
RefSeqiNP_438231.1. NC_000907.1.
WP_005693864.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VH3X-ray2.70A/B/C2-254[»]
1VICX-ray1.80A/B2-254[»]
3DUVX-ray2.30A/B1-254[»]
ProteinModelPortaliP44490.
SMRiP44490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21736; AAC21736; HI_0058.
GeneIDi950957.
KEGGihin:HI0058.
PATRICi20188569. VBIHaeInf48452_0058.

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
KOiK00979.
OMAiNSGTERC.
PhylomeDBiP44490.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00358; UER00476.
BRENDAi2.7.7.38. 2529.

Miscellaneous databases

EvolutionaryTraceiP44490.

Family and domain databases

CDDicd02517. CMP-KDO-Synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. KdsB. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKDSB_HAEIN
AccessioniPrimary (citable) accession number: P44490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.