Reviewed, UniProtKB/Swiss-Prot P44470 (RLMH_HAEIN)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase H
    EC=2.1.1.-
Alternative name(s):
    rRNA (pseudouridine-N3-)-methyltransferase rlmH
    23S rRNA m3Psi1915 methyltransferase

Gene names

Name:	rlmH
Ordered Locus Names:	HI0033

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N3-methylpseudouridine. HAMAP MF_00658
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase rlmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (pseudouridine-N3-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Ribosomal RNA large subunit methyltransferase H HAMAP MF_00658		PRO_0000198126

Sequences

	Sequence		Length	Mass (Da)
	P44470-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D147B9FA24389A1E	FASTA	155	17,250
	10 20 30 40 50 60 MKITLIAVGT KMPSWVTTGF EEYQRRFPKD MPFELIEIPA GKRGKNADIK RILEQEGKAM 70 80 90 100 110 120 LAACGKGKVV TLDIPGKPWT TPQLAEQLEA WKNDGRDVCL LIGGPEGLSP ECKAAAEQSW 130 140 150 SLSPLTLPHP LVRVVVAESL YRAWSLTTNH PYHRE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21711.1.
PIR	G64140.
RefSeq	NP_438206.1.
3D structure databases
SMR	P44470. Positions 1-155.
ModBase	Search...
Genome annotation databases
GeneID	950930.
GenomeReviews	Gene locus HI0033 in contig L42023_GR.
KEGG	hin:HI0033.
TIGR	HI0033.
Phylogenomic databases
HOGENOM	P44470.
OMA	IVTLEVH
Enzyme and pathway databases
BioCyc	HINF71421:HI_0033-MON.
Family and domain databases
HAMAP	MF_00658. [Tree]
InterPro	IPR016051. CHP00246. IPR003742. SPOUT_MeTrfase. [Graphical view]
Pfam	PF02590. SPOUT_MTase. 1 hit. [Graphical view]
PIRSF	PIRSF004505. MT_bac. 1 hit.
TIGRFAMs	TIGR00246. DUF163. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMH_HAEIN

Accession

Primary (citable) accession number: P44470

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)