Reviewed, UniProtKB/Swiss-Prot P44469 (PBP2_HAEIN)

Names and origin

Protein names

Recommended name:
    Penicillin-binding protein 2
      Short name=PBP-2

Gene names

Name:	mrdA
Synonyms:	pbp2
Ordered Locus Names:	HI0032

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross-linked peptidoglycan from the lipid intermediates By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell inner membrane By similarity.
Domain	Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalytic activity Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Penicillin-binding protein 2		PRO_0000195448
Sites
	Active site	338	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44469-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4BEB73290952AAB0	FASTA	651	73,813
	10 20 30 40 50 60 MNLKKFFNTP THEPFRDKKA ERNLFARRTL VAFLGILLLT GVLFTNIYQL QIVNFDTYQT 70 80 90 100 110 120 RSNGNRIKLL PLPPTRGLIY DRYGKLLAEN LTFFGLYIVP EKTENLDRTL DELRYIVGLT 130 140 150 160 170 180 DNDIENFKKE RRRGTRYTPI LLKPNLTEEQ ISRFAVNGYQ YPSLEVRPYF KRHYLYGETM 190 200 210 220 230 240 AHILGYVGKM NDKDVERLKR EDKFANYAGT NDIGKLGIER YYEDILQGTT GFEEVEINNR 250 260 270 280 290 300 GKVIRTLRSR PAVAGKSIHL TIDLALQRYI TELLSGLKGA VVVLDPKDSS VLAMVSTPSY 310 320 330 340 350 360 DNNLFVDGIS SEDYKRLLND LARPLYSRAT QGAYPPASTV KPFIAVAAQT ENVITPNTTI 370 380 390 400 410 420 FDPGYWVLPN STKRFRDWKK TGHGDTDLNK AITESSDTYF YQVAYNMGID RLSNWMKDFG 430 440 450 460 470 480 FGMPTGIEIQ EETAANIPTR EWKQKRYKRP WVQGDTISVG IGQGYWTATP LQVAKATTIL 490 500 510 520 530 540 VNNGKVNTPH LMKAIEGAVL EPYEDPLLYP DINTPKVAAW EAAKRGMYNV VNAANGTGRK 550 560 570 580 590 600 AFADANYRVA GKSGTAQVFS LKENEKYNTA GLKKELHDHA WFTAYAPYDN PKLVVTVILE 610 620 630 640 650 NAGGGSSNAA PLARKVMDYY LNQRLPQVEK YNVPIQQKKD LSQESEQING R

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21710.1.
PIR	C64044.
RefSeq	NP_438205.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950929.
GenomeReviews	Gene locus HI0032 in contig L42023_GR.
KEGG	hin:HI0032.
TIGR	HI0032.
Phylogenomic databases
HOGENOM	P44469.
OMA	MTHALGY
Enzyme and pathway databases
BioCyc	HINF71421:HI_0032-MON.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. IPR017790. Penicillin-binding_protein_2. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03423. pbp2_mrdA. 1 hit.
ProtoNet	Search...
Other Resources
DrugBank	DB00303. Ertapenem.

Entry information

Entry name

PBP2_HAEIN

Accession

Primary (citable) accession number: P44469

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)