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Protein

Peptidoglycan D,D-transpeptidase MrdA

Gene

mrdA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall.UniRule annotation

Catalytic activityi

Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei338Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-32-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase MrdAUniRule annotation (EC:3.4.16.4UniRule annotation)
Alternative name(s):
Penicillin-binding protein 2UniRule annotation
Short name:
PBP-2UniRule annotation
Gene namesi
Name:mrdAUniRule annotation
Synonyms:pbp2
Ordered Locus Names:HI_0032
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB00671 Cefixime
DB00303 Ertapenem

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954481 – 651Peptidoglycan D,D-transpeptidase MrdAAdd BLAST651

Proteomic databases

PRIDEiP44469

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0032

Structurei

3D structure databases

ProteinModelPortaliP44469
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similaritiesi

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN Bacteria
COG0768 LUCA
KOiK05515
OMAiSCDTYYY
PhylomeDBiP44469

Family and domain databases

HAMAPiMF_02081 MrdA_transpept, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR005311 PBP_dimer
IPR036138 PBP_dimer_sf
IPR001460 PCN-bd_Tpept
IPR017790 Penicillin-binding_protein_2
PfamiView protein in Pfam
PF03717 PBP_dimer, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF56519 SSF56519, 1 hit
SSF56601 SSF56601, 1 hit
TIGRFAMsiTIGR03423 pbp2_mrdA, 1 hit

Sequencei

Sequence statusi: Complete.

P44469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKKFFNTP THEPFRDKKA ERNLFARRTL VAFLGILLLT GVLFTNIYQL
60 70 80 90 100
QIVNFDTYQT RSNGNRIKLL PLPPTRGLIY DRYGKLLAEN LTFFGLYIVP
110 120 130 140 150
EKTENLDRTL DELRYIVGLT DNDIENFKKE RRRGTRYTPI LLKPNLTEEQ
160 170 180 190 200
ISRFAVNGYQ YPSLEVRPYF KRHYLYGETM AHILGYVGKM NDKDVERLKR
210 220 230 240 250
EDKFANYAGT NDIGKLGIER YYEDILQGTT GFEEVEINNR GKVIRTLRSR
260 270 280 290 300
PAVAGKSIHL TIDLALQRYI TELLSGLKGA VVVLDPKDSS VLAMVSTPSY
310 320 330 340 350
DNNLFVDGIS SEDYKRLLND LARPLYSRAT QGAYPPASTV KPFIAVAAQT
360 370 380 390 400
ENVITPNTTI FDPGYWVLPN STKRFRDWKK TGHGDTDLNK AITESSDTYF
410 420 430 440 450
YQVAYNMGID RLSNWMKDFG FGMPTGIEIQ EETAANIPTR EWKQKRYKRP
460 470 480 490 500
WVQGDTISVG IGQGYWTATP LQVAKATTIL VNNGKVNTPH LMKAIEGAVL
510 520 530 540 550
EPYEDPLLYP DINTPKVAAW EAAKRGMYNV VNAANGTGRK AFADANYRVA
560 570 580 590 600
GKSGTAQVFS LKENEKYNTA GLKKELHDHA WFTAYAPYDN PKLVVTVILE
610 620 630 640 650
NAGGGSSNAA PLARKVMDYY LNQRLPQVEK YNVPIQQKKD LSQESEQING

R
Length:651
Mass (Da):73,813
Last modified:November 1, 1995 - v1
Checksum:i4BEB73290952AAB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC21710.1
PIRiC64044
RefSeqiNP_438205.1, NC_000907.1
WP_005649850.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC21710; AAC21710; HI_0032
GeneIDi950929
KEGGihin:HI0032
PATRICifig|71421.8.peg.32

Entry informationi

Entry nameiMRDA_HAEIN
AccessioniPrimary (citable) accession number: P44469
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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