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Protein

Peptidoglycan D,D-transpeptidase MrdA

Gene

mrdA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall.UniRule annotation

Catalytic activityi

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei338Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase MrdAUniRule annotation (EC:3.4.16.4UniRule annotation)
Alternative name(s):
Penicillin-binding protein 2UniRule annotation
Short name:
PBP-2UniRule annotation
Gene namesi
Name:mrdAUniRule annotation
Synonyms:pbp2
Ordered Locus Names:HI_0032
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB00671. Cefixime.
DB00303. Ertapenem.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954481 – 651Peptidoglycan D,D-transpeptidase MrdAAdd BLAST651

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0032.

Structurei

3D structure databases

ProteinModelPortaliP44469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has a penicillin insensitive transglycosylase domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase domain (cross-linking of the peptide subunits).

Sequence similaritiesi

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
KOiK05515.
OMAiWYDGETI.
PhylomeDBiP44469.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_02081. MrdA_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

P44469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKKFFNTP THEPFRDKKA ERNLFARRTL VAFLGILLLT GVLFTNIYQL
60 70 80 90 100
QIVNFDTYQT RSNGNRIKLL PLPPTRGLIY DRYGKLLAEN LTFFGLYIVP
110 120 130 140 150
EKTENLDRTL DELRYIVGLT DNDIENFKKE RRRGTRYTPI LLKPNLTEEQ
160 170 180 190 200
ISRFAVNGYQ YPSLEVRPYF KRHYLYGETM AHILGYVGKM NDKDVERLKR
210 220 230 240 250
EDKFANYAGT NDIGKLGIER YYEDILQGTT GFEEVEINNR GKVIRTLRSR
260 270 280 290 300
PAVAGKSIHL TIDLALQRYI TELLSGLKGA VVVLDPKDSS VLAMVSTPSY
310 320 330 340 350
DNNLFVDGIS SEDYKRLLND LARPLYSRAT QGAYPPASTV KPFIAVAAQT
360 370 380 390 400
ENVITPNTTI FDPGYWVLPN STKRFRDWKK TGHGDTDLNK AITESSDTYF
410 420 430 440 450
YQVAYNMGID RLSNWMKDFG FGMPTGIEIQ EETAANIPTR EWKQKRYKRP
460 470 480 490 500
WVQGDTISVG IGQGYWTATP LQVAKATTIL VNNGKVNTPH LMKAIEGAVL
510 520 530 540 550
EPYEDPLLYP DINTPKVAAW EAAKRGMYNV VNAANGTGRK AFADANYRVA
560 570 580 590 600
GKSGTAQVFS LKENEKYNTA GLKKELHDHA WFTAYAPYDN PKLVVTVILE
610 620 630 640 650
NAGGGSSNAA PLARKVMDYY LNQRLPQVEK YNVPIQQKKD LSQESEQING

R
Length:651
Mass (Da):73,813
Last modified:November 1, 1995 - v1
Checksum:i4BEB73290952AAB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21710.1.
PIRiC64044.
RefSeqiNP_438205.1. NC_000907.1.
WP_005649850.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21710; AAC21710; HI_0032.
GeneIDi950929.
KEGGihin:HI0032.
PATRICi20188515. VBIHaeInf48452_0032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21710.1.
PIRiC64044.
RefSeqiNP_438205.1. NC_000907.1.
WP_005649850.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP44469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0032.

Chemistry databases

DrugBankiDB00671. Cefixime.
DB00303. Ertapenem.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21710; AAC21710; HI_0032.
GeneIDi950929.
KEGGihin:HI0032.
PATRICi20188515. VBIHaeInf48452_0032.

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
KOiK05515.
OMAiWYDGETI.
PhylomeDBiP44469.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_02081. MrdA_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMRDA_HAEIN
AccessioniPrimary (citable) accession number: P44469
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 15, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.