Reviewed, UniProtKB/Swiss-Prot P44466 (DACA_HAEIN)

Names and origin

Protein names

Recommended name:
    D-alanyl-D-alanine carboxypeptidase dacA
      Short name=DD-carboxypeptidase
      Short name=DD-peptidase
    EC=3.4.16.4
Alternative name(s):
    Penicillin-binding protein 5
      Short name=PBP-5

Gene names

Name:	dacA
Ordered Locus Names:	HI0029

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors By similarity.
Catalytic activity	Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity. Note: N-terminal lies in the periplasmic space By similarity.
Sequence similarities	Belongs to the peptidase S11 family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal
Molecular function	Carboxypeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type D-Ala-D-Ala carboxypeptidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 393	375	D-alanyl-D-alanine carboxypeptidase dacA		PRO_0000027232
Sites
	Active site	65	1	Acyl-ester intermediate By similarity
	Active site	68	1	Proton acceptor By similarity
	Active site	128	1	By similarity
	Binding site	231	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44466-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 31DF0C563A833B7C	FASTA	393	43,414
	10 20 30 40 50 60 MLKRTTKIAF LSSFVALSAF SVSAEDMQFG VTPPQITAQT YVLMDYNSGA ILTALNPDQR 70 80 90 100 110 120 QYPASLTKMM TSYVVGVALK QGKIHNTDMV TIGESAWGRN FPDSSKMFLD LNTQVSVADL 130 140 150 160 170 180 NRGVIVVSGN DATVALAEHI SGNVPNFVET MNKYVQQFGL KNTNFTTPHG LDDPNQYSSA 190 200 210 220 230 240 RDMAIIGAHI IRDLPEEYKI YSEKNFTFNK IKQANRNGLL WDKTINVDGM KTGHTSQAGY 250 260 270 280 290 300 NLVASATTSN NMRLISVVMG VPTYKGREVE SKKLLQWGFA NFETFKTLEA GKEISEQRVY 310 320 330 340 350 360 YGDKNSVKLG ALMDHFITIP KGKQSEVKAR YELADKNLQA PLVKGQVIGK VVYQLDGKDI 370 380 390 ASANLQVMND VGEAGIFGKL WDWLVLTVKG LFS

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21707.1.
PIR	I64043.
RefSeq	NP_438202.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950922.
GenomeReviews	Gene locus HI0029 in contig L42023_GR.
KEGG	hin:HI0029.
NMPDR	fig|71421.1.peg.28.
TIGR	HI0029.
Phylogenomic databases
HOGENOM	HBG728991.
OMA	GKIHNTD.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0029-MON.
BRENDA	3.4.16.4. 109.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR015956. Peniciliin-bd_prot-assoc. IPR001967. Peptidase_S11. IPR012907. Peptidase_S11_C. IPR018044. Peptidase_S11_cons-region. [Graphical view]
Gene3D	G3DSA:2.60.410.10. Peptidase_S11_C. 1 hit.
Pfam	PF07943. PBP5_C. 1 hit. PF00768. Peptidase_S11. 1 hit. [Graphical view]
PRINTS	PR00725. DADACBPTASE1.
ProtoNet	Search...
Other Resources
DrugBank	DB00303. Ertapenem.

Entry information

Entry name

DACA_HAEIN

Accession

Primary (citable) accession number: P44466

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)