Reviewed,
UniProtKB/Swiss-Prot P44463 (LIPA_HAEIN)
Last modified
December 15, 2009.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Lipoyl synthase EC=2.8.1.8 Alternative name(s): Lipoic acid synthase Lipoate synthase Sulfur insertion protein lipA Lip-syn Short name=LS | ||||
| Gene names |
| ||||
| Organism | Haemophilus influenzae [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 727 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus |
Protein attributes
| Sequence length | 320 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. |
| Catalytic activity | Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP MF_00206 |
| Cofactor | Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. |
| Pathway | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP MF_00206 |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the radical SAM superfamily. Lipoyl synthase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine |
| Molecular function | Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | lipoate biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW lipoate synthase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 320 | 320 | Lipoyl synthase HAMAP MF_00206 | PRO_0000102320 | |||||
Sites | |||||||||
| Metal binding | 67 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 72 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 78 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 93 | 1 | Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity | ||||||
| Metal binding | 97 | 1 | Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity | ||||||
| Metal binding | 100 | 1 | Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity | ||||||
Sequences
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References
| [1] | "Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. Venter J.C.Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd. |
Cross-references
Sequence databases | |
|---|---|
| L42023 Genomic DNA. Translation: AAC21704.1. | |
| PIR | G64043. |
| RefSeq | NP_438199.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 950923. |
| GenomeReviews | Gene locus HI0026 in contig L42023_GR. |
| KEGG | hin:HI0026. |
| TIGR | HI0026. |
Phylogenomic databases | |
| HOGENOM | HBG284542. |
| OMA | TTIEVLI. |
Enzyme and pathway databases | |
| BioCyc | HINF71421:HI_0026-MON. |
| BRENDA | 2.8.1.8. 109. |
Family and domain databases | |
| HAMAP | MF_00206. [Tree] |
| InterPro | IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoate_synth. IPR007197. Radical_SAM. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. |
| Pfam | PF04055. Radical_SAM. 1 hit. [Graphical view] |
| PIRSF | PIRSF005963. Lipoyl_synth. 1 hit. |
| SMART | SM00729. Elp3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LIPA_HAEIN | ||||||||
| Accession | Primary (citable) accession number: P44463 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Haemophilus influenzae Haemophilus influenzae (strain Rd): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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