Reviewed, UniProtKB/Swiss-Prot P44463 (LIPA_HAEIN)

Names and origin

Protein names

Recommended name:
    Lipoyl synthase
    EC=2.8.1.8
Alternative name(s):
    Lipoic acid synthase
    Lipoate synthase
    Sulfur insertion protein lipA
    Lip-syn
      Short name=LS

Gene names

Name:	lipA
Ordered Locus Names:	HI0026

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP MF_00206
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipoate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Lipoyl synthase HAMAP MF_00206		PRO_0000102320
Sites
	Metal binding	67	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	72	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	93	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	97	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	100	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44463-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7D4C4136925450C6	FASTA	320	36,197
	10 20 30 40 50 60 MSTPFKMERG VKYRDAAKTS IIPVKNIDPN QDLLKKPEWM KIKLPASSAK IESIKNGMRR 70 80 90 100 110 120 HGLHSVCEEA SCPNLHECFN HGTATFMILG AICTRRCPFC DVAHGKPLPP DPEEPQKLAE 130 140 150 160 170 180 TIQDMKLKYV VITSVDRDDL PDRGAGHFSE CVKAVRELNP NIKIEILVPD FRGRITQALE 190 200 210 220 230 240 KLKDNPPDVF NHNLENVPRL YKEIRPGADY EWSLKLLREF KEIFPNIPTK SGLMVGLGET 250 260 270 280 290 300 NEEILQVMQD LRDNGVTMLT LGQYLQPSRH HLPVARYVPP TEFDEFRDKA NEMGFEHAAC 310 320 GPFVRSSYHA DLQASGGLVK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21704.1.
PIR	G64043.
RefSeq	NP_438199.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950923.
GenomeReviews	Gene locus HI0026 in contig L42023_GR.
KEGG	hin:HI0026.
TIGR	HI0026.
Phylogenomic databases
HOGENOM	HBG284542.
OMA	TTIEVLI.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0026-MON.
BRENDA	2.8.1.8. 109.
Family and domain databases
HAMAP	MF_00206. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoate_synth. IPR007197. Radical_SAM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPA_HAEIN

Accession

Primary (citable) accession number: P44463

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)