P44450 (FDXH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate dehydrogenase iron-sulfur subunit

Alternative name(s):
Formate dehydrogenase subunit beta
Short name=FDH subunit beta

Gene names

Name:	fdxH
Ordered Locus Names:	HI_0007

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit By similarity.
Subunit structure	Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.
Subcellular location	Cell membrane By similarity.
Sequence similarities	Contains 4 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Formate dehydrogenase iron-sulfur subunit		PRO_0000159252
Regions
	Domain	35 – 65	31	4Fe-4S ferredoxin-type 1
	Domain	97 – 129	33	4Fe-4S ferredoxin-type 2
	Domain	130 – 159	30	4Fe-4S ferredoxin-type 3
	Domain	164 – 195	32	4Fe-4S ferredoxin-type 4
Sites
	Metal binding	44	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	47	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	54	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	106	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	109	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	114	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	118	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	139	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	142	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	145	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	166	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	169	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	181	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	185	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44450 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AA49DD3C17064866	FASTA	312	34,069
	10 20 30 40 50 60 MAGTAQGVQT QDVIKISATS GLTPAPQARD HKVEIAKLID VSTCIGCKAC QVGCSEWNDI 70 80 90 100 110 120 RSDINAQCVG VYDNPVDLDA KAWTVMRFNE VEENDRLEWL IRKDGCMHCT EPGCLKACPA 130 140 150 160 170 180 PGAIIQYANG IVDFQSDKCI GCGYCIAGCP FNIPRMNPED NRVYKCTLCV DRVSVGQEPA 190 200 210 220 230 240 CVKTCPTGAI RFGSKEEMKI YAEQRVADLK SRGYENAGLY DPPGVGGTHV MYVLHHADKP 250 260 270 280 290 300 ELYNGLPKDP QIDLSVTLWK DVLKPVAAVA MGGLALAEVA HYLTVGPNVE EDVEDHHHEF 310 EENKPSKGEN NE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21685.1.
PIR	A64042.
RefSeq	NP_438180.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44450.
SMR	P44450. Positions 7-292.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC21685; AAC21685; HI_0007.
GeneID	950904.
KEGG	hin:HI0007.
PATRIC	20188465. VBIHaeInf48452_0007.
Phylogenomic databases
eggNOG	COG0437.
KO	K00124.
OMA	PRARDYK.
ProtClustDB	CLSK870084.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR006470. Formate_DH_bsu. IPR014603. Formate_DH_Fe-S_su. IPR015246. Formate_DH_TM. [Graphical view]
Pfam	PF13247. Fer4_11. 1 hit. PF12800. Fer4_4. 1 hit. PF09163. Form-deh_trans. 1 hit. [Graphical view]
PIRSF	PIRSF036298. FDH_4Fe4S. 1 hit.
TIGRFAMs	TIGR01582. FDH-beta. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDXH_HAEIN

Accession

Primary (citable) accession number: P44450

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program