Reviewed, UniProtKB/Swiss-Prot P44439 (PCNB_HAEIN)

Names and origin

Protein names

Recommended name:
    Probable poly(A) polymerase
      Short name=PAP
    EC=2.7.7.19

Gene names

Name:	pcnB
Ordered Locus Names:	HI0063

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Polymerase that creates the 3' poly(A) tail found in some mRNA's By similarity.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA polyadenylation Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Probable poly(A) polymerase		PRO_0000139093
Sites
	Active site	68	1	By similarity
	Active site	70	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44439-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1B3A9F9E2286F349	FASTA	452	52,698
	10 20 30 40 50 60 MPKARAKKSE QTRRYDKNVI KAAQFDISPR DFSRNALNVV EKLQRQGFEA YIVGGCIRDL 70 80 90 100 110 120 LLGKKPKDFD VATNARPEQI QNIFQRQCRL VGRRFRLAHI MFGRDIIEVA TFRANHSDAR 130 140 150 160 170 180 NENQAKQSNE GMLLRDNVYG TIEQDAARRD FTVNALYYNP QDNTLRDYFE GIKDLKAGKL 190 200 210 220 230 240 RLIGDPVTRY QEDPVRMLRS IRFMAKLDMF LEKPSEQPIR ELAPLLKNIP PARLFDESLK 250 260 270 280 290 300 LLQAGQGVKT YRLLRQYGLF EQLFPALSAY FTEKEDSFAE RMIVTALTST DERVADKLRI 310 320 330 340 350 360 NPAFLFAAFF WYPLREKVEI LKNEGGLNNH DAYALAGNEV LDLFCRALAA PRRHTAVIRD 370 380 390 400 410 420 IWFLQLQLLK RTGSAPMRTM EHPKFRAGFD LLAMRAEIEG GETIELAKWW HEYQFSNGEQ 430 440 450 REQLIQEQQR LHPKPKKKYY RPRRRKTTCS AE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21741.1.
PIR	B64046.
RefSeq	NP_438236.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950961.
GenomeReviews	Gene locus HI0063 in contig L42023_GR.
KEGG	hin:HI0063.
NMPDR	fig|71421.1.peg.62.
TIGR	HI0063.
Phylogenomic databases
HOGENOM	P44439.
OMA	WYPLREK
Enzyme and pathway databases
BioCyc	HINF71421:HI_0063-MON.
BRENDA	2.7.7.19. 109.
Family and domain databases
InterPro	IPR010206. PolA_pol_PcnB. IPR002646. PolyA_pol_reg. [Graphical view]
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
TIGRFAMs	TIGR01942. pcnB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PCNB_HAEIN

Accession

Primary (citable) accession number: P44439

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)