P44436 (HIS6_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisF
EC=4.1.3.-

Alternative name(s):
IGP synthase cyclase subunit
IGP synthase subunit HisF
ImGP synthase subunit HisF
Short name=IGPS subunit HisF

Gene names

Name:	hisF
Ordered Locus Names:	HI_0474

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit By similarity. HAMAP MF_01013
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_01013
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_01013
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_01013
Subcellular location	Cytoplasm By similarity HAMAP MF_01013.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Imidazole glycerol phosphate synthase subunit HisF HAMAP MF_01013		PRO_0000142163
Sites
	Active site	11	1	Potential
	Active site	130	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P44436 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: ABC2F6351F1642C2	FASTA	258	28,626
	10 20 30 40 50 60 MLAKRIIPCL DVRDGQVVKG VQFRNHEIIG DIVPLAQRYA QEGADELVFY DITASSDGRT 70 80 90 100 110 120 VDKSWVERIA QVIDIPFCVA GGIKTIEDAE KLFAFGADKI SINSPALADP TLISRLADRF 130 140 150 160 170 180 GVQAIVVGID SWFEQETGKY WVNQYTGDET RTRQTHWQLL DWVKEVQQCG AGEIVLNMMN 190 200 210 220 230 240 QDGLRNGYDL AQLKLVRGVC RVPLIASGGA GKMVHFRDAF IEAKVDGALA ASVFHKQIIE 250 IGELKSYLVQ SAIEIRSE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22133.1.
PIR	I64070.
RefSeq	NP_438635.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44436.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949565.
GenomeReviews	Gene locus HI_0474 in contig L42023_GR.
KEGG	hin:HI0474.
PATRIC	20189503. VBIHaeInf48452_0494.
TIGR	HI_0474.
Phylogenomic databases
HOGENOM	HBG541613.
OMA	ASVFHKG.
PhylomeDB	P44436.
ProtClustDB	PRK02083.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0474-MONOMER.
Family and domain databases
HAMAP	MF_01013. HisF. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR004651. HisF. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K02500.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00735. HisF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS6_HAEIN

Accession

Primary (citable) accession number: P44436

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program