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Protein

DNA adenine methylase

Gene

dam

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211S-adenosyl-L-methionineBy similarity
Binding sitei25 – 251S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei66 – 661S-adenosyl-L-methionineBy similarity
Binding sitei194 – 1941S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi1152. M.HindDam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.HindIV
Gene namesi
Name:dam
Synonyms:hindIVM
Ordered Locus Names:HI_0209
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286DNA adenine methylasePRO_0000087996Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0209.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.
PhylomeDBiP44431.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPKKQSLK PKLKHRPFLK WAGGKFRLTD EINKAFPNKK NCLIEPFVGA
60 70 80 90 100
GAVFLNSNFE RYILADINPD LINLFNIVKX NVDGYIEDCK PIFFADDANT
110 120 130 140 150
PDYYYAKRRQ FNASTEPFER SIIFLYLNRF GFNGLCRYNS KNEFNVPFGA
160 170 180 190 200
YKTHYFPEDE LRYFAHKAQS AVFLCCDFQK TFEFADKDSV IYCDPPYAPL
210 220 230 240 250
QQETNFTGYA GNEFGLAQQR ALADLAKSIQ KEKQISILIS NHDTKFTREI
260 270 280
YNGAKFKRVK VQRSISQNPE KRVKVKELIA IFGARK
Length:286
Mass (Da):33,219
Last modified:November 1, 1995 - v1
Checksum:iBE39C5725AE35DEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21877.1.
PIRiH64054.
RefSeqiNP_438378.1. NC_000907.1.
WP_010868957.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21877; AAC21877; HI_0209.
GeneIDi951117.
KEGGihin:HI0209.
PATRICi20188915. VBIHaeInf48452_0214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21877.1.
PIRiH64054.
RefSeqiNP_438378.1. NC_000907.1.
WP_010868957.1. NC_000907.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0209.

Protein family/group databases

REBASEi1152. M.HindDam.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21877; AAC21877; HI_0209.
GeneIDi951117.
KEGGihin:HI0209.
PATRICi20188915. VBIHaeInf48452_0214.

Phylogenomic databases

eggNOGiCOG0338.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.
PhylomeDBiP44431.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiDMA_HAEIN
AccessioniPrimary (citable) accession number: P44431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.