Reviewed, UniProtKB/Swiss-Prot P44431 (DMA_HAEIN)

Names and origin

Protein names

Recommended name:
    DNA adenine methylase
    EC=2.1.1.72
Alternative name(s):
    M.HindIV
    Deoxyadenosyl-methyltransferase
    DNA adenine methyltransferase

Gene names

Name:	dam
Synonyms:	hindIVM
Ordered Locus Names:	HI0209

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	DNA adenine methylase		PRO_0000087996
Sites
	Binding site	21	1	S-adenosyl-L-methionine By similarity
	Binding site	25	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	194	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44431-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BE39C5725AE35DEE	FASTA	286	33,219
	10 20 30 40 50 60 MLRPKKQSLK PKLKHRPFLK WAGGKFRLTD EINKAFPNKK NCLIEPFVGA GAVFLNSNFE 70 80 90 100 110 120 RYILADINPD LINLFNIVKX NVDGYIEDCK PIFFADDANT PDYYYAKRRQ FNASTEPFER 130 140 150 160 170 180 SIIFLYLNRF GFNGLCRYNS KNEFNVPFGA YKTHYFPEDE LRYFAHKAQS AVFLCCDFQK 190 200 210 220 230 240 TFEFADKDSV IYCDPPYAPL QQETNFTGYA GNEFGLAQQR ALADLAKSIQ KEKQISILIS 250 260 270 280 NHDTKFTREI YNGAKFKRVK VQRSISQNPE KRVKVKELIA IFGARK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21877.1.
PIR	H64054.
RefSeq	NP_438378.1.
3D structure databases
HSSP	HSSP built from PDB template 2DPM based on UniProtKB P04043.
ModBase	Search...
Genome annotation databases
GeneID	951117.
GenomeReviews	Gene locus HI0209 in contig L42023_GR.
KEGG	hin:HI0209.
NMPDR	fig|71421.1.peg.199.
TIGR	HI0209.
Phylogenomic databases
HOGENOM	P44431.
OMA	WAGGKFR.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0209-MON.
BRENDA	2.1.1.72. 109.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_HAEIN

Accession

Primary (citable) accession number: P44431

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)