Malate dehydrogenase - Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

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P44427 (MDH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	HI_1210

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus ›

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 311

311

Malate dehydrogenase HAMAP-Rule MF_01516

PRO_0000113306

Regions

Nucleotide binding

7 – 13

NAD By similarity

Nucleotide binding

117 – 119

NAD By similarity

Sites

Active site

177

Proton acceptor By similarity

Binding site

NAD By similarity

Binding site

Substrate By similarity

Binding site

Substrate By similarity

Binding site

NAD By similarity

Binding site

119

Substrate By similarity

Binding site

153

Substrate By similarity

Binding site

227

NAD By similarity

Natural variations

Natural variant

P → L in strain: 767 and 794.

Natural variant

T → I in strain: 432.

Natural variant

A → T in strain: 1124.

Natural variant

107 – 108

VT → TI in strain: 773, 796 and 1231.

Natural variant

107

V → I in strain: 162, 375, 723, 788, 800, 1008, 1124, 1158, 1180, 1181, 1207, 1233, 1247, a6062, e6158, e6229, e7066, f6237 and 1209.

Natural variant

107

V → T in strain: a7205, BR355 and e6181.

Natural variant

128

A → V in strain: c6132.

Natural variant

135

A → S in strain: a7205.

Natural variant

163

G → D in strain: f6252.

Natural variant

193

K → E in strain: 176, 667, a7205, b7017, c1271, c6134, c7424 and c8032.

Sequences

Sequence

Length

Mass (Da)

Tools

P44427 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1311441B90E4EA2C
Show »

FASTA

311

32,543

        10         20         30         40         50         60 
MKVAVLGAAG GIGQALALLL KLQLPAGTDL SLYDIAPVTP GVAVDVSHIP TAVNVKGFSG 

        70         80         90        100        110        120 
EDPTPALEGA DVVLISAGVA RKPGMDRSDL FNINAGIVRG LIEKVAVTCP KACVGIITNP 

       130        140        150        160        170        180 
VNTTVAIAAE VLKKAGVYDK RKLFGVTTLD VLRSETFVAE LKGLNVSRTS VPVIGGHSGV 

       190        200        210        220        230        240 
TILPLLSQVQ YAKWNEDEIE PLTKRIQNAG TEVLNAKAGG GSATLSMAQA AARFARSLVK 

       250        260        270        280        290        300 
GLSGETVVEC TYVEGDGKYA RFFSQPVRLG KEGVEEILPI GPLSNFEQQA LENMLPTLRA 

       310 
DIELGEKFIN G

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Recombination within natural populations of pathogenic bacteria: Short-term empirical estimates and long-term phylogenetic consequences." Feil E.J., Holmes E.C., Bessen D.E., Chan M.-S., Day N.P.J., Enright M.C., Goldstein R., Hood D.W., Kalia A., Moore C.E., Zhou J., Spratt B.G. Proc. Natl. Acad. Sci. U.S.A. 98:182-187(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195. Strain: 1124, 1158, 1181, 1209, 375, 477, 667, 723, a1042, a6062, a6064, a6073, a7190, a7205, b1324, b6107, b7017, b7109, b7651, b7717, Beagan, c1271, c6132, c6134, c7424, c8032, d6137, Drm118, e6158, e6181, e6229, e7066, f6237, f6252, f7283 and f7290.
[3]	"High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae." Cody A.J., Field D., Feil E.J., Stringer S., Deadman M.E., Tsolaki A.G., Gratz B., Bouchet V., Goldstein R., Hood D.W., Moxon E.R. Infect. Genet. Evol. 3:57-66(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195. Strain: 1008, 1180, 1200, 1207, 1231, 1233, 1247, 1268, 162, 176, 285, 432, 486 and 981.
[4]	"Invasive disease due to nontypeable Haemophilus influenzae among children in Arkansas." O'Neill J.M., St Geme J.W. III, Cutter D., Adderson E.E., Anyanwu J., Jacobs R.F., Schutze G.E. J. Clin. Microbiol. 41:3064-3069(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195. Strain: 767, 769, 773, 788, 794, 795, 796 and 800.
[5]	"Multilocus sequence-typing for characterization of Moscow strains of Haemophilus influenzae type b." Platonov A.E., Mironov K.O., Iatsyshina S.B., Koroleva I.S., Platonova O.V., Gushchin A.E., Shipulin G.A. Mol. Genet. Mikrobiol. Virusol. 2:21-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195. Strain: BR134, BR229, BR355, BR48 and BRC1-13.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22864.1. AF322740 Genomic DNA. Translation: AAK11394.1. AF322741 Genomic DNA. Translation: AAK11395.1. AF322742 Genomic DNA. Translation: AAK11396.1. AF322743 Genomic DNA. Translation: AAK11397.1. AF322744 Genomic DNA. Translation: AAK11398.1. AF322745 Genomic DNA. Translation: AAK11399.1. AF322746 Genomic DNA. Translation: AAK11400.1. AF322747 Genomic DNA. Translation: AAK11401.1. AF322748 Genomic DNA. Translation: AAK11402.1. AF322749 Genomic DNA. Translation: AAK11403.1. AF322750 Genomic DNA. Translation: AAK11404.1. AF322751 Genomic DNA. Translation: AAK11405.1. AF322752 Genomic DNA. Translation: AAK11406.1. AF322753 Genomic DNA. Translation: AAK11407.1. AF322754 Genomic DNA. Translation: AAK11408.1. AF322755 Genomic DNA. Translation: AAK11409.1. AF322756 Genomic DNA. Translation: AAK11410.1. AF322757 Genomic DNA. Translation: AAK11411.1. AF322758 Genomic DNA. Translation: AAK11412.1. AF322759 Genomic DNA. Translation: AAK11413.1. AF322760 Genomic DNA. Translation: AAK11414.1. AF322761 Genomic DNA. Translation: AAK11415.1. AF322762 Genomic DNA. Translation: AAK11416.1. AF322763 Genomic DNA. Translation: AAK11417.1. AF322764 Genomic DNA. Translation: AAK11418.1. AF322765 Genomic DNA. Translation: AAK11419.1. AF322766 Genomic DNA. Translation: AAK11420.1. AF322767 Genomic DNA. Translation: AAK11421.1. AF322768 Genomic DNA. Translation: AAK11422.1. AF322769 Genomic DNA. Translation: AAK11423.1. AF322770 Genomic DNA. Translation: AAK11424.1. AF322771 Genomic DNA. Translation: AAK11425.1. AF322772 Genomic DNA. Translation: AAK11426.1. AF322773 Genomic DNA. Translation: AAK11427.1. AF322774 Genomic DNA. Translation: AAK11428.1. AF322775 Genomic DNA. Translation: AAK11429.1. AF322776 Genomic DNA. Translation: AAK11430.1. AF536037 Genomic DNA. Translation: AAP19893.1. AF536038 Genomic DNA. Translation: AAP19894.1. AF536039 Genomic DNA. Translation: AAP19895.1. AF536040 Genomic DNA. Translation: AAP19896.1. AF536041 Genomic DNA. Translation: AAP19897.1. AF536042 Genomic DNA. Translation: AAP19898.1. AF536043 Genomic DNA. Translation: AAP19899.1. AF536044 Genomic DNA. Translation: AAP19900.1. AF536045 Genomic DNA. Translation: AAP19901.1. AF536046 Genomic DNA. Translation: AAP19902.1. AF536047 Genomic DNA. Translation: AAP19903.1. AF536048 Genomic DNA. Translation: AAP19904.1. AF536049 Genomic DNA. Translation: AAP19905.1. AF536050 Genomic DNA. Translation: AAP19906.1. AY245392 Genomic DNA. Translation: AAP74406.1. AY245393 Genomic DNA. Translation: AAP74407.1. AY245394 Genomic DNA. Translation: AAP74408.1. AY245395 Genomic DNA. Translation: AAP74409.1. AY245396 Genomic DNA. Translation: AAP74410.1. AY245397 Genomic DNA. Translation: AAP74411.1. AY245398 Genomic DNA. Translation: AAP74412.1. AY245399 Genomic DNA. Translation: AAP74413.1. AF525726 Genomic DNA. Translation: AAM91960.1. AF525727 Genomic DNA. Translation: AAM91961.1. AF525728 Genomic DNA. Translation: AAM91962.1. AF525729 Genomic DNA. Translation: AAM91963.1. AF525730 Genomic DNA. Translation: AAM91964.1.
PIR	C64110.
RefSeq	NP_439366.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44427.
SMR	P44427. Positions 1-310.
ModBase	Search...
Proteomic databases
PRIDE	P44427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22864; AAC22864; HI_1210.
GeneID	950148.
KEGG	hin:HI1210.
PATRIC	20191099. VBIHaeInf48452_1262.
Phylogenomic databases
KO	K00024.
OMA	NVIECSY.
ProtClustDB	PRK05086.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_HAEIN

Accession

Primary (citable) accession number: P44427
Secondary accession number(s): Q7WRT7

Q9AMQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents