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Protein

Malate dehydrogenase

Gene

mdh

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei81SubstrateBy similarity1
Binding sitei87SubstrateBy similarity1
Binding sitei94NADBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei153SubstrateBy similarity1
Active sitei177Proton acceptorBy similarity1
Binding sitei227NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 13NADBy similarity7
Nucleotide bindingi117 – 119NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-1241-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:HI_1210
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133061 – 311Malate dehydrogenaseAdd BLAST311

Proteomic databases

PRIDEiP44427

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1210

Structurei

Secondary structure

1311
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 23Combined sources13
Beta strandi29 – 33Combined sources5
Helixi39 – 47Combined sources9
Beta strandi54 – 58Combined sources5
Helixi64 – 67Combined sources4
Beta strandi71 – 75Combined sources5
Helixi87 – 90Combined sources4
Helixi91 – 108Combined sources18
Beta strandi112 – 116Combined sources5
Beta strandi118 – 120Combined sources3
Helixi121 – 134Combined sources14
Helixi140 – 142Combined sources3
Beta strandi143 – 145Combined sources3
Helixi148 – 162Combined sources15
Turni166 – 168Combined sources3
Beta strandi173 – 175Combined sources3
Helixi179 – 181Combined sources3
Beta strandi182 – 184Combined sources3
Helixi186 – 188Combined sources3
Turni196 – 198Combined sources3
Helixi199 – 207Combined sources9
Helixi209 – 216Combined sources8
Turni217 – 219Combined sources3
Helixi225 – 242Combined sources18
Beta strandi247 – 254Combined sources8
Beta strandi261 – 270Combined sources10
Beta strandi273 – 277Combined sources5
Helixi285 – 309Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6AOOX-ray2.15A/B1-311[»]
6BALX-ray2.10A/B/C/D/E/F/G/H1-311[»]
ProteinModelPortaliP44427
SMRiP44427
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
KOiK00024
OMAiQCTPKVE
PhylomeDBiP44427

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P44427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KLQLPAGTDL SLYDIAPVTP GVAVDVSHIP
60 70 80 90 100
TAVNVKGFSG EDPTPALEGA DVVLISAGVA RKPGMDRSDL FNINAGIVRG
110 120 130 140 150
LIEKVAVTCP KACVGIITNP VNTTVAIAAE VLKKAGVYDK RKLFGVTTLD
160 170 180 190 200
VLRSETFVAE LKGLNVSRTS VPVIGGHSGV TILPLLSQVQ YAKWNEDEIE
210 220 230 240 250
PLTKRIQNAG TEVLNAKAGG GSATLSMAQA AARFARSLVK GLSGETVVEC
260 270 280 290 300
TYVEGDGKYA RFFSQPVRLG KEGVEEILPI GPLSNFEQQA LENMLPTLRA
310
DIELGEKFIN G
Length:311
Mass (Da):32,543
Last modified:November 1, 1995 - v1
Checksum:i1311441B90E4EA2C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti63P → L in strain: 767 and 794. 1
Natural varianti64T → I in strain: 432. 1
Natural varianti66A → T in strain: 1124. 1
Natural varianti107 – 108VT → TI in strain: 773, 796 and 1231. 2
Natural varianti107V → I in strain: 162, 375, 723, 788, 800, 1008, 1124, 1158, 1180, 1181, 1207, 1233, 1247, a6062, e6158, e6229, e7066, f6237 and 1209. 1
Natural varianti107V → T in strain: a7205, BR355 and e6181. 1
Natural varianti128A → V in strain: c6132. 1
Natural varianti135A → S in strain: a7205. 1
Natural varianti163G → D in strain: f6252. 1
Natural varianti193K → E in strain: 176, 667, a7205, b7017, c1271, c6134, c7424 and c8032. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22864.1
AF322740 Genomic DNA Translation: AAK11394.1
AF322741 Genomic DNA Translation: AAK11395.1
AF322742 Genomic DNA Translation: AAK11396.1
AF322743 Genomic DNA Translation: AAK11397.1
AF322744 Genomic DNA Translation: AAK11398.1
AF322745 Genomic DNA Translation: AAK11399.1
AF322746 Genomic DNA Translation: AAK11400.1
AF322747 Genomic DNA Translation: AAK11401.1
AF322748 Genomic DNA Translation: AAK11402.1
AF322749 Genomic DNA Translation: AAK11403.1
AF322750 Genomic DNA Translation: AAK11404.1
AF322751 Genomic DNA Translation: AAK11405.1
AF322752 Genomic DNA Translation: AAK11406.1
AF322753 Genomic DNA Translation: AAK11407.1
AF322754 Genomic DNA Translation: AAK11408.1
AF322755 Genomic DNA Translation: AAK11409.1
AF322756 Genomic DNA Translation: AAK11410.1
AF322757 Genomic DNA Translation: AAK11411.1
AF322758 Genomic DNA Translation: AAK11412.1
AF322759 Genomic DNA Translation: AAK11413.1
AF322760 Genomic DNA Translation: AAK11414.1
AF322761 Genomic DNA Translation: AAK11415.1
AF322762 Genomic DNA Translation: AAK11416.1
AF322763 Genomic DNA Translation: AAK11417.1
AF322764 Genomic DNA Translation: AAK11418.1
AF322765 Genomic DNA Translation: AAK11419.1
AF322766 Genomic DNA Translation: AAK11420.1
AF322767 Genomic DNA Translation: AAK11421.1
AF322768 Genomic DNA Translation: AAK11422.1
AF322769 Genomic DNA Translation: AAK11423.1
AF322770 Genomic DNA Translation: AAK11424.1
AF322771 Genomic DNA Translation: AAK11425.1
AF322772 Genomic DNA Translation: AAK11426.1
AF322773 Genomic DNA Translation: AAK11427.1
AF322774 Genomic DNA Translation: AAK11428.1
AF322775 Genomic DNA Translation: AAK11429.1
AF322776 Genomic DNA Translation: AAK11430.1
AF536037 Genomic DNA Translation: AAP19893.1
AF536038 Genomic DNA Translation: AAP19894.1
AF536039 Genomic DNA Translation: AAP19895.1
AF536040 Genomic DNA Translation: AAP19896.1
AF536041 Genomic DNA Translation: AAP19897.1
AF536042 Genomic DNA Translation: AAP19898.1
AF536043 Genomic DNA Translation: AAP19899.1
AF536044 Genomic DNA Translation: AAP19900.1
AF536045 Genomic DNA Translation: AAP19901.1
AF536046 Genomic DNA Translation: AAP19902.1
AF536047 Genomic DNA Translation: AAP19903.1
AF536048 Genomic DNA Translation: AAP19904.1
AF536049 Genomic DNA Translation: AAP19905.1
AF536050 Genomic DNA Translation: AAP19906.1
AY245392 Genomic DNA Translation: AAP74406.1
AY245393 Genomic DNA Translation: AAP74407.1
AY245394 Genomic DNA Translation: AAP74408.1
AY245395 Genomic DNA Translation: AAP74409.1
AY245396 Genomic DNA Translation: AAP74410.1
AY245397 Genomic DNA Translation: AAP74411.1
AY245398 Genomic DNA Translation: AAP74412.1
AY245399 Genomic DNA Translation: AAP74413.1
AF525726 Genomic DNA Translation: AAM91960.1
AF525727 Genomic DNA Translation: AAM91961.1
AF525728 Genomic DNA Translation: AAM91962.1
AF525729 Genomic DNA Translation: AAM91963.1
AF525730 Genomic DNA Translation: AAM91964.1
PIRiC64110
RefSeqiNP_439366.1, NC_000907.1
WP_005694235.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22864; AAC22864; HI_1210
GeneIDi950148
KEGGihin:HI1210
PATRICifig|71421.8.peg.1262

Similar proteinsi

Entry informationi

Entry nameiMDH_HAEIN
AccessioniPrimary (citable) accession number: P44427
Secondary accession number(s): Q7WRT7
, Q83V59, Q83V60, Q99Q89, Q99Q90, Q99QA9, Q9AMQ2, Q9AMQ3, Q9AMQ4, Q9AMQ5, Q9AMQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health