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P44427 (MDH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:HI_1210
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01516

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Malate dehydrogenase HAMAP-Rule MF_01516
PRO_0000113306

Regions

Nucleotide binding7 – 137NAD By similarity
Nucleotide binding117 – 1193NAD By similarity

Sites

Active site1771Proton acceptor By similarity
Binding site341NAD By similarity
Binding site811Substrate By similarity
Binding site871Substrate By similarity
Binding site941NAD By similarity
Binding site1191Substrate By similarity
Binding site1531Substrate By similarity
Binding site2271NAD By similarity

Natural variations

Natural variant631P → L in strain: 767 and 794.
Natural variant641T → I in strain: 432.
Natural variant661A → T in strain: 1124.
Natural variant107 – 1082VT → TI in strain: 773, 796 and 1231.
Natural variant1071V → I in strain: 162, 375, 723, 788, 800, 1008, 1124, 1158, 1180, 1181, 1207, 1233, 1247, a6062, e6158, e6229, e7066, f6237 and 1209.
Natural variant1071V → T in strain: a7205, BR355 and e6181.
Natural variant1281A → V in strain: c6132.
Natural variant1351A → S in strain: a7205.
Natural variant1631G → D in strain: f6252.
Natural variant1931K → E in strain: 176, 667, a7205, b7017, c1271, c6134, c7424 and c8032.

Sequences

Sequence LengthMass (Da)Tools
P44427 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1311441B90E4EA2C

FASTA31132,543
        10         20         30         40         50         60 
MKVAVLGAAG GIGQALALLL KLQLPAGTDL SLYDIAPVTP GVAVDVSHIP TAVNVKGFSG 

        70         80         90        100        110        120 
EDPTPALEGA DVVLISAGVA RKPGMDRSDL FNINAGIVRG LIEKVAVTCP KACVGIITNP 

       130        140        150        160        170        180 
VNTTVAIAAE VLKKAGVYDK RKLFGVTTLD VLRSETFVAE LKGLNVSRTS VPVIGGHSGV 

       190        200        210        220        230        240 
TILPLLSQVQ YAKWNEDEIE PLTKRIQNAG TEVLNAKAGG GSATLSMAQA AARFARSLVK 

       250        260        270        280        290        300 
GLSGETVVEC TYVEGDGKYA RFFSQPVRLG KEGVEEILPI GPLSNFEQQA LENMLPTLRA 

       310 
DIELGEKFIN G 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd."
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. expand/collapse author list , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C.
Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]"Recombination within natural populations of pathogenic bacteria: Short-term empirical estimates and long-term phylogenetic consequences."
Feil E.J., Holmes E.C., Bessen D.E., Chan M.-S., Day N.P.J., Enright M.C., Goldstein R., Hood D.W., Kalia A., Moore C.E., Zhou J., Spratt B.G.
Proc. Natl. Acad. Sci. U.S.A. 98:182-187(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195.
Strain: 1124, 1158, 1181, 1209, 375, 477, 667, 723, a1042, a6062, a6064, a6073, a7190, a7205, b1324, b6107, b7017, b7109, b7651, b7717, Beagan, c1271, c6132, c6134, c7424, c8032, d6137, Drm118, e6158, e6181, e6229, e7066, f6237, f6252, f7283 and f7290.
[3]"High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae."
Cody A.J., Field D., Feil E.J., Stringer S., Deadman M.E., Tsolaki A.G., Gratz B., Bouchet V., Goldstein R., Hood D.W., Moxon E.R.
Infect. Genet. Evol. 3:57-66(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195.
Strain: 1008, 1180, 1200, 1207, 1231, 1233, 1247, 1268, 162, 176, 285, 432, 486 and 981.
[4]"Invasive disease due to nontypeable Haemophilus influenzae among children in Arkansas."
O'Neill J.M., St Geme J.W. III, Cutter D., Adderson E.E., Anyanwu J., Jacobs R.F., Schutze G.E.
J. Clin. Microbiol. 41:3064-3069(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195.
Strain: 767, 769, 773, 788, 794, 795, 796 and 800.
[5]"Multilocus sequence-typing for characterization of Moscow strains of Haemophilus influenzae type b."
Platonov A.E., Mironov K.O., Iatsyshina S.B., Koroleva I.S., Platonova O.V., Gushchin A.E., Shipulin G.A.
Mol. Genet. Mikrobiol. Virusol. 2:21-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-195.
Strain: BR134, BR229, BR355, BR48 and BRC1-13.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22864.1.
AF322740 Genomic DNA. Translation: AAK11394.1.
AF322741 Genomic DNA. Translation: AAK11395.1.
AF322742 Genomic DNA. Translation: AAK11396.1.
AF322743 Genomic DNA. Translation: AAK11397.1.
AF322744 Genomic DNA. Translation: AAK11398.1.
AF322745 Genomic DNA. Translation: AAK11399.1.
AF322746 Genomic DNA. Translation: AAK11400.1.
AF322747 Genomic DNA. Translation: AAK11401.1.
AF322748 Genomic DNA. Translation: AAK11402.1.
AF322749 Genomic DNA. Translation: AAK11403.1.
AF322750 Genomic DNA. Translation: AAK11404.1.
AF322751 Genomic DNA. Translation: AAK11405.1.
AF322752 Genomic DNA. Translation: AAK11406.1.
AF322753 Genomic DNA. Translation: AAK11407.1.
AF322754 Genomic DNA. Translation: AAK11408.1.
AF322755 Genomic DNA. Translation: AAK11409.1.
AF322756 Genomic DNA. Translation: AAK11410.1.
AF322757 Genomic DNA. Translation: AAK11411.1.
AF322758 Genomic DNA. Translation: AAK11412.1.
AF322759 Genomic DNA. Translation: AAK11413.1.
AF322760 Genomic DNA. Translation: AAK11414.1.
AF322761 Genomic DNA. Translation: AAK11415.1.
AF322762 Genomic DNA. Translation: AAK11416.1.
AF322763 Genomic DNA. Translation: AAK11417.1.
AF322764 Genomic DNA. Translation: AAK11418.1.
AF322765 Genomic DNA. Translation: AAK11419.1.
AF322766 Genomic DNA. Translation: AAK11420.1.
AF322767 Genomic DNA. Translation: AAK11421.1.
AF322768 Genomic DNA. Translation: AAK11422.1.
AF322769 Genomic DNA. Translation: AAK11423.1.
AF322770 Genomic DNA. Translation: AAK11424.1.
AF322771 Genomic DNA. Translation: AAK11425.1.
AF322772 Genomic DNA. Translation: AAK11426.1.
AF322773 Genomic DNA. Translation: AAK11427.1.
AF322774 Genomic DNA. Translation: AAK11428.1.
AF322775 Genomic DNA. Translation: AAK11429.1.
AF322776 Genomic DNA. Translation: AAK11430.1.
AF536037 Genomic DNA. Translation: AAP19893.1.
AF536038 Genomic DNA. Translation: AAP19894.1.
AF536039 Genomic DNA. Translation: AAP19895.1.
AF536040 Genomic DNA. Translation: AAP19896.1.
AF536041 Genomic DNA. Translation: AAP19897.1.
AF536042 Genomic DNA. Translation: AAP19898.1.
AF536043 Genomic DNA. Translation: AAP19899.1.
AF536044 Genomic DNA. Translation: AAP19900.1.
AF536045 Genomic DNA. Translation: AAP19901.1.
AF536046 Genomic DNA. Translation: AAP19902.1.
AF536047 Genomic DNA. Translation: AAP19903.1.
AF536048 Genomic DNA. Translation: AAP19904.1.
AF536049 Genomic DNA. Translation: AAP19905.1.
AF536050 Genomic DNA. Translation: AAP19906.1.
AY245392 Genomic DNA. Translation: AAP74406.1.
AY245393 Genomic DNA. Translation: AAP74407.1.
AY245394 Genomic DNA. Translation: AAP74408.1.
AY245395 Genomic DNA. Translation: AAP74409.1.
AY245396 Genomic DNA. Translation: AAP74410.1.
AY245397 Genomic DNA. Translation: AAP74411.1.
AY245398 Genomic DNA. Translation: AAP74412.1.
AY245399 Genomic DNA. Translation: AAP74413.1.
AF525726 Genomic DNA. Translation: AAM91960.1.
AF525727 Genomic DNA. Translation: AAM91961.1.
AF525728 Genomic DNA. Translation: AAM91962.1.
AF525729 Genomic DNA. Translation: AAM91963.1.
AF525730 Genomic DNA. Translation: AAM91964.1.
PIRC64110.
RefSeqNP_439366.1. NC_000907.1.

3D structure databases

ProteinModelPortalP44427.
SMRP44427. Positions 1-310.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP44427.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22864; AAC22864; HI_1210.
GeneID950148.
KEGGhin:HI1210.
PATRIC20191099. VBIHaeInf48452_1262.

Phylogenomic databases

KOK00024.
OMAKARGVYN.
OrthoDBEOG6091FG.
PhylomeDBP44427.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01516. Malate_dehydrog_1.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_HAEIN
AccessionPrimary (citable) accession number: P44427
Secondary accession number(s): Q7WRT7 expand/collapse secondary AC list , Q83V59, Q83V60, Q99Q89, Q99Q90, Q99QA9, Q9AMQ2, Q9AMQ3, Q9AMQ4, Q9AMQ5, Q9AMQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names