Reviewed, UniProtKB/Swiss-Prot P44414 (MTHD_HAEIN)

Names and origin

Protein names

Recommended name:
    Probable modification methylase HindII
      Short name=M.HindII
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase HindII

Gene names

Name:	hindIIM
Ordered Locus Names:	HI0513

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HindII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Probable modification methylase HindII		PRO_0000087974

Sequences

	Sequence		Length	Mass (Da)
	P44414-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2E0CAA5B9DBF2EF6	FASTA	518	60,410
	10 20 30 40 50 60 MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ LGYTNLTSYE 70 80 90 100 110 120 IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL SELQKKELKD NSIWKMYCNS 130 140 150 160 170 180 LCDYFYIFII KSILQLKVGG ELIFICPDYF FSTKNAEGLR KFLINNGSFE KIILFNESKV 190 200 210 220 230 240 FHGVSSSVVI FKYIKGKNID NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP 250 260 270 280 290 300 NHIKVALDKF ESYCKTIKKV QPKSLFDDLE FSRIGSVCDI GNGMVSGLDK AFQMNDINYS 310 320 330 340 350 360 ELELLNSICV AKAKHLDAFC FSGYTRYKFI LDDINEDKLI TYFPNFFYEF NNYKDYLLKR 370 380 390 400 410 420 YSYNKYLPYW KWAFLRNFSL FSKNEKKIFV PCKERISKKS NFRFSLVDEF IYPTQDVTAL 430 440 450 460 470 480 YKKENVKESI EYITAYLNSK AVFLWMKYKG VVKGNVVEFS EKPLANIPFR RIDWQLKSEK 490 500 510 KIHDDITNLV RKYLSNKEFS ILHEINLNLE KLGIKVEI

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22171.1.
PIR	F64073.
RefSeq	NP_438671.1.
3D structure databases
HSSP	HSSP built from PDB template 2ADM based on UniProtKB P14385.
ModBase	Search...
Genome annotation databases
GeneID	950799.
GenomeReviews	Gene locus HI0513 in contig L42023_GR.
KEGG	hin:HI0513.
NMPDR	fig|71421.1.peg.484.
TIGR	HI0513.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0513-MON.
BRENDA	2.1.1.72. 109.
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHD_HAEIN

Accession

Primary (citable) accession number: P44414

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)