P44413 (T2D2_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HindII
Short name=R.HindII
EC=3.1.21.4

Alternative name(s):
Endonuclease HindII
Type II restriction enzyme HindII

Gene names

Name:	hindIIR
Ordered Locus Names:	HI_0512

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 258	257	Type-2 restriction enzyme HindII		PRO_0000077320

Sequences

	Sequence		Length	Mass (Da)
	P44413 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6BE104A1D1B63238	FASTA	258	29,929
	10 20 30 40 50 60 MSFIKPIYQD INSILIGQKV KRPKSGTLSG HAAGEPFEKL VYKFLKENLS DLTFKQYEYL 70 80 90 100 110 120 NDLFMKKPAI IGHEARYKLF NSPTLLFLLS RGKAATENWS IENLFEEKQN DTADILLVKD 130 140 150 160 170 180 QFYELLDVKT RNISKSAQAP NIISAYKLAQ TCAKMIDNKE FDLFDINYLE VDWELNGEDL 190 200 210 220 230 240 VCVSTSFAEL FKSEPSELYI NWAAAMQIQF HVRDLDQGFN GTREEWAKSY LKHFVTQAEQ 250 RAISMIDKFV KPFKKYIL

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22170.1.
PIR	E64073.
RefSeq	NP_438670.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44413.
SMR	P44413. Positions 2-258.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0512.
Protein family/group databases
REBASE	1150. HindII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22170; AAC22170; HI_0512.
GeneID	950710.
KEGG	hin:HI0512.
PATRIC	20189577. VBIHaeInf48452_0531.
Phylogenomic databases
eggNOG	NOG320573.
KO	K01155.
OMA	NIISAYK.
ProtClustDB	CLSK2518359.
Family and domain databases
Gene3D	3.40.600.10. 1 hit.
InterPro	IPR011337. DNA_rep_MutH/RE_typeII. IPR011335. Restrct_endonuc-II-like. IPR015307. Restrct_endonuc_II_HincII. [Graphical view]
Pfam	PF09226. Endonuc-HincII. 1 hit. [Graphical view]
SUPFAM	SSF52980. Restrict_endonuc_II-like_core. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2D2_HAEIN

Accession

Primary (citable) accession number: P44413

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program