Reviewed, UniProtKB/Swiss-Prot P44413 (T2D2_HAEIN)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme HindII
      Short name=R.HindII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme HindII
    Endonuclease HindII

Gene names

Name:	hindIIR
Ordered Locus Names:	HI0512

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 258

257

Type-2 restriction enzyme HindII

PRO_0000077320

Secondary structure

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258

Helix Strand Turn

Details...

Turn

4 – 7

4

Helix

8 – 15

8

Helix

36 – 38

3

Helix

39 – 44

6

Turn

48 – 52

5

Helix

56 – 65

10

Helix

73 – 78

6

Helix

83 – 89

7

Helix

93 – 98

6

Beta strand

101 – 103

3

Beta strand

114 – 119

6

Beta strand

122 – 131

10

Beta strand

141 – 144

4

Helix

145 – 158

14

Beta strand

161 – 175

15

Beta strand

180 – 189

10

Helix

190 – 192

3

Helix

195 – 197

3

Beta strand

200 – 202

3

Beta strand

207 – 209

3

Turn

212 – 214

3

Helix

223 – 249

27

Turn

250 – 253

4

Helix

254 – 257

4

Sequences

	Sequence		Length	Mass (Da)
	P44413-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6BE104A1D1B63238	FASTA	258	29,929
	10 20 30 40 50 60 MSFIKPIYQD INSILIGQKV KRPKSGTLSG HAAGEPFEKL VYKFLKENLS DLTFKQYEYL 70 80 90 100 110 120 NDLFMKKPAI IGHEARYKLF NSPTLLFLLS RGKAATENWS IENLFEEKQN DTADILLVKD 130 140 150 160 170 180 QFYELLDVKT RNISKSAQAP NIISAYKLAQ TCAKMIDNKE FDLFDINYLE VDWELNGEDL 190 200 210 220 230 240 VCVSTSFAEL FKSEPSELYI NWAAAMQIQF HVRDLDQGFN GTREEWAKSY LKHFVTQAEQ 250 RAISMIDKFV KPFKKYIL

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L42023 Genomic DNA. Translation: AAC22170.1.

PIR

E64073.

RefSeq

NP_438670.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TW8	X-ray	2.80	A/B/C/D	2-257	[»]
1TX3	X-ray	2.50	A/B/C/D	2-257	[»]
2AUD	X-ray	2.10	A	2-257	[»]
2GIE	X-ray	2.60	A/B/C/D	2-258	[»]
2GIG	X-ray	1.83	A/B	2-258	[»]
2GIH	X-ray	2.50	A/B	2-258	[»]
2GII	X-ray	2.30	A/B	2-258	[»]
2GIJ	X-ray	1.93	A/B	2-258	[»]
3E3Y	X-ray	2.13	A/B	2-258	[»]
3E40	X-ray	2.10	A/B	2-258	[»]
3E41	X-ray	2.73	A/B	2-258	[»]
3E42	X-ray	2.68	A/B	2-258	[»]
3E43	X-ray	2.73	A/B	2-258	[»]
3E44	X-ray	2.52	A/B	2-258	[»]
3E45	X-ray	2.78	A/B	2-258	[»]
3EBC	X-ray	2.55	A/B	1-258	[»]

ModBase

Search...

Genome annotation databases

GeneID

950710.

GenomeReviews

Gene locus HI0512 in contig L42023_GR.

KEGG

hin:HI0512.

NMPDR

fig|71421.1.peg.483.

TIGR

HI0512.

Enzyme and pathway databases

BioCyc

HINF71421:HI_0512-MON.

BRENDA

3.1.21.4. 109.

Family and domain databases

InterPro

IPR011337. Restrict_endonuc_II/DNA_repair.
IPR015307. Restrict_endonuc_II_HincII.
[Graphical view]

Gene3D

G3DSA:3.40.600.10. Restrict_endonuc_II/DNA_repair. 1 hit.

Pfam

PF09226. Endonuc-HincII. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2D2_HAEIN

Accession

Primary (citable) accession number: P44413

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	July 28, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)