Reviewed, UniProtKB/Swiss-Prot P44407 (MSBA_HAEIN)

Names and origin

Protein names

Recommended name:
    Lipid A export ATP-binding/permease protein msbA
    EC=3.6.3.-

Gene names

Name:	msbA
Synonyms:	msh-1
Ordered Locus Names:	HI0060

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Domain	In msbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused. HAMAP MF_01703
Sequence similarities	Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid transport Inferred from electronic annotation. Source: HAMAP transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro lipid transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Lipid A export ATP-binding/permease protein msbA HAMAP MF_01703		PRO_0000092583
Regions
	Transmembrane	31 – 51	21	Potential
	Transmembrane	68 – 88	21	Potential
	Transmembrane	145 – 165	21	Potential
	Transmembrane	166 – 186	21	Potential
	Transmembrane	259 – 279	21	Potential
	Domain	31 – 315	285	ABC transmembrane type-1
	Domain	347 – 583	237	ABC transporter
	Nucleotide binding	381 – 388	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44407-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 97F90D5537436123	FASTA	587	64,912
	10 20 30 40 50 60 MQEQKLQEND FSTLQTFKRL WPMIKPFKAG LIVSGVALVF NALADSGLIY LLKPLLDDGF 70 80 90 100 110 120 GKANHSFLKM MAFVVVGMII LRGITNFISN YCLAWVSGKV VMTMRRRLFK HLMFMPVSFF 130 140 150 160 170 180 DQNSTGRLLS RITYDSQMIA SSSSGSLITI VREGAYIISL FAVMFYTSWE LTIVLFIIGP 190 200 210 220 230 240 IIAVLIRLVS KIFRRLSKNL QDSMGELTSA TEQMLKGHKV VLSFGGQHVE EVHFNHVSND 250 260 270 280 290 300 MRRKSMKMVT ANSISDPVVQ VIASLALATV LYLATTPLIA EDNLSAGSFT VVFSSMLAMM 310 320 330 340 350 360 RPLKSLTAVN AQFQSGMAAC QTLFAILDLE PEKDDGAYKA EPAKGELEFK NVSFAYQGKD 370 380 390 400 410 420 ELALNNISFS VPAGKTVALV GRSGSGKSTI ANLVTRFYDI EQGEILLDGV NIQDYRLSNL 430 440 450 460 470 480 RENCAVVSQQ VHLFNDTIAN NIAYAAQDKY SREEIIAAAK AAYALEFIEK LPQVFDTVIG 490 500 510 520 530 540 ENGTSLSGGQ RQRLAIARAL LRNSPVLILD EATSALDTES ERAIQSALEE LKKDRTVVVI 550 560 570 580 AHRLSTIENA DEILVIDHGE IRERGNHKTL LEQNGAYKQL HSMQFTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Sequence of the rec-2 locus of Haemophilus influenzae: homologies to comE-ORF3 of Bacillus subtilis and msbA of Escherichia coli." Clifton S.W., McCarthy D., Roe B.A. Gene 146:95-100(1994) [PubMed: 8063112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-458. Strain: BC200.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21738.1. L20805 Genomic DNA. Translation: AAC13734.1.
PIR	H64045.
RefSeq	NP_438233.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950953.
GenomeReviews	Gene locus HI0060 in contig L42023_GR.
KEGG	hin:HI0060.
NMPDR	fig|71421.1.peg.59.
TIGR	HI0060.
Phylogenomic databases
HOGENOM	P44407.
OMA	LTNVNSQ
Enzyme and pathway databases
BioCyc	HINF71421:HI_0060-MON.
Family and domain databases
HAMAP	MF_01703. [Tree]
InterPro	IPR011527. ABC_TM_1. IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR011917. ABC_transpr_lipidA. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02203. MsbA_lipidA. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51239. MSBA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSBA_HAEIN

Accession

Primary (citable) accession number: P44407

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)