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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18By similarity1
Active sitei150By similarity1
Metal bindingi257Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 1UniRule annotation1
Metal bindingi270Magnesium or manganese 2UniRule annotation1
Metal bindingi272Magnesium or manganese 2UniRule annotation1
Active sitei281By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 189ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-1173-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Synonyms:ddlB
Ordered Locus Names:HI_1140
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778271 – 306D-alanine--D-alanine ligaseAdd BLAST306

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1140

Structurei

3D structure databases

ProteinModelPortaliP44405
SMRiP44405
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 303ATP-graspUniRule annotationAdd BLAST200

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
KOiK01921
OMAiYETKYTE
PhylomeDBiP44405

Family and domain databases

Gene3Di3.30.1490.201 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P44405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLKQEKIAV LLGGTSAERE VSLNSGKAVL EALLKQGYNA HPIDPKEYNV
60 70 80 90 100
ANLKKDGFNR AFNILHGRGG EDGTMQGLLE QIGLPYTGCG VMASALTMDK
110 120 130 140 150
MRTKMLWKAF GLPVADMKVV TRETFSELDP QAVVAKLGLP LMVKPSLEGS
160 170 180 190 200
SVGLTKVKAV EELKSAVEYA LKFDNTILIE EWLAGDELTV PVLDNQVLPA
210 220 230 240 250
IRIVPEGEFY DYEAKYISDN TQYFCPAGLT PEREQALSTL VKRAYDAVGC
260 270 280 290 300
RGWSRIDVMC DAKGNFRLVE VNTNPGMTSH SLFPKSAATV GISFEQLVVK

ILELSL
Length:306
Mass (Da):33,491
Last modified:November 1, 1995 - v1
Checksum:iC20B30CB540E6D7A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22795.1
PIRiF64185
RefSeqiNP_439298.1, NC_000907.1
WP_005693454.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22795; AAC22795; HI_1140
GeneIDi950555
KEGGihin:HI1140
PATRICifig|71421.8.peg.1190

Similar proteinsi

Entry informationi

Entry nameiDDL_HAEIN
AccessioniPrimary (citable) accession number: P44405
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome