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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851NucleophileBy similarity
Active sitei179 – 1791By similarity
Active sitei181 – 1811By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi233 – 2397ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. GMP synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. pyrophosphatase activity Source: InterPro

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-HAMAP
  2. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:HI_0222
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523GMP synthase [glutamine-hydrolyzing]PRO_0000140131Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0222.

Structurei

3D structure databases

ProteinModelPortaliP44335.
SMRiP44335. Positions 3-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 205198Glutamine amidotransferase type-1Add
BLAST
Domaini206 – 398193GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0519.
KOiK01951.
OMAiKNAKWLA.
OrthoDBiEOG6JHRJV.
PhylomeDBiP44335.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNIHYHKIL ILDFGSQYTQ LIARRVREIG VYCELWAWDV TEQQIREFAP
60 70 80 90 100
TGIILSGSPE STTEENSPRA PEYVFNAGVP VLGICYGMQT MAMQLGGLTE
110 120 130 140 150
TSDHREFGYA SVSLENSTAL FANLNDNLTA SEPKLDVWMS HGDKVTRLPE
160 170 180 190 200
NFKVTGTTLT CPIAAMSDES RRFYGVQFHP EVTHTKKGLE LLTNFVVNIC
210 220 230 240 250
GCETKWTAEN IIEDAVARIK EQVGDDEVIL GLSGGVDSSV VALLLHRAIG
260 270 280 290 300
KNLHCVFVDN GLLRLHEGDQ VMEMFGDKFG LNITRVDAES RFLGELAGVS
310 320 330 340 350
DPEAKRKIIG KVFVDVFDDE SKKLTNVKWL AQGTIYPDVI ESAASKTGKA
360 370 380 390 400
HVIKSHHNVG GLPDYMKLGL VEPLRELFKD EVRKIGLALG LPAEMINRHP
410 420 430 440 450
FPGPGLGVRV LGEVKKEYCD LLRRADAIFI EELRNSGWYE KTSQAFSVFL
460 470 480 490 500
PVKSVGVMGD GRKYDWVISL RAVETIDFMT AHWAHLPYDL LGKVSNRIIN
510 520
EVNGISRVVY DISGKPPATI EWE
Length:523
Mass (Da):58,186
Last modified:November 1, 1995 - v1
Checksum:i6361F38BC20CF154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21891.1.
PIRiI64055.
RefSeqiNP_438394.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21891; AAC21891; HI_0222.
GeneIDi951140.
KEGGihin:HI0222.
PATRICi20188965. VBIHaeInf48452_0235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21891.1.
PIRiI64055.
RefSeqiNP_438394.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP44335.
SMRiP44335. Positions 3-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21891; AAC21891; HI_0222.
GeneIDi951140.
KEGGihin:HI0222.
PATRICi20188965. VBIHaeInf48452_0235.

Phylogenomic databases

eggNOGiCOG0519.
KOiK01951.
OMAiKNAKWLA.
OrthoDBiEOG6JHRJV.
PhylomeDBiP44335.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiGUAA_HAEIN
AccessioniPrimary (citable) accession number: P44335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.