P44334 (IMDH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosine-5'-monophosphate dehydrogenase
Short name=IMP dehydrogenase
Short name=IMPD
Short name=IMPDH
EC=1.1.1.205

Gene names

Name:	guaB
Ordered Locus Names:	HI_0221

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.
Pathway	Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. Contains 2 CBS domains.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	CBS domain Repeat
Ligand	Metal-binding NAD Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Inosine-5'-monophosphate dehydrogenase		PRO_0000093697
Regions
	Domain	94 – 150	57	CBS 1
	Domain	154 – 215	62	CBS 2
	Nucleotide binding	228 – 251	24	NAD By similarity
Sites
	Active site	306	1	Thioimidate intermediate By similarity
	Metal binding	301	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	303	1	Potassium; via carbonyl oxygen By similarity
	Binding site	339	1	IMP By similarity
	Binding site	386	1	IMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44334 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B477F22344E4BE8B	FASTA	488	51,981
	10 20 30 40 50 60 MSLRIKQEAL TFDDVLLVPA HSTVLPNTAN LSTQLTKEIR LNIPMLSAAM DTVTETKLAI 70 80 90 100 110 120 SLAQEGGIGF IHKNMTIERQ ADRVRKVKKF ESGIVSEPVT VLPNLTLAEL AEMVKKNGFA 130 140 150 160 170 180 GYPVVDGENN LIGIITGRDT RFVKDLSKTV SQVMTKKEDL VTVKEGASRE EILELMHQHR 190 200 210 220 230 240 VEKVLVVNDS FKLKGMITVK DFQKAEQKPN ACKDEFGRLR VGAAVGAGAG NEERIDALVK 250 260 270 280 290 300 AGVDVLLIDS SHGHSEGVLQ RVRETRAKYP NLPIVAGNVA TAEGAIALAD AGASAVKVGI 310 320 330 340 350 360 GPGSICTTRI VTGVGVPQIT AIADAAAALK DRGIPVIADG GIRFSGDIAK AIAAGASCVM 370 380 390 400 410 420 VGSMFAGTEE APGEIELYQG RAFKSYRGMG SLGAMAKGSS DRYFQSDNAA DKLVPEGIEG 430 440 450 460 470 480 RIPYKGYLKE IIHQQMGGLR SCMGLTGCAT IDELRTKAEF VRISGAGIKE SHVHDVAITK EAPNYRMG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21890.1.
PIR	H64055.
RefSeq	NP_438392.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44334.
SMR	P44334. Positions 3-486.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	951137.
GenomeReviews	Gene locus HI_0221 in contig L42023_GR.
KEGG	hin:HI0221.
NMPDR	fig|71421.1.peg.210.
PATRIC	20188961. VBIHaeInf48452_0233.
TIGR	HI_0221.
Phylogenomic databases
HOGENOM	HBG298985.
OMA	GIGIVHK.
PhylomeDB	P44334.
ProtClustDB	PRK05567.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0221-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000644. Cysta_beta_synth_core. IPR005990. IMP_DH. IPR018529. IMP_DH-rel. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00088.
PANTHER	PTHR11911:SF6. IMP_DH_GMPRtase. 1 hit.
Pfam	PF00571. CBS. 2 hits. PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000130. IMPDH. 1 hit.
SMART	SM00116. CBS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01302. IMP_dehydrog. 1 hit.
PROSITE	PS51371. CBS. 2 hits. PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMDH_HAEIN

Accession

Primary (citable) accession number: P44334

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program