Reviewed, UniProtKB/Swiss-Prot P44318 (EX3_HAEIN)

Names and origin

Protein names

Recommended name:
    Exodeoxyribonuclease III
      Short name=Exonuclease III
      Short name=EXO III
    EC=3.1.11.2

Gene names

Name:	xthA
Ordered Locus Names:	HI0041

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA repair enzymes AP/exoA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Exodeoxyribonuclease III		PRO_0000200022
Sites
	Active site	259	1	Proton acceptor By similarity
	Metal binding	34	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44318-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6B3ADE465A1E347C	FASTA	267	31,025
	10 20 30 40 50 60 MKFISFNING LRARPHQLEA IIEKYQPDVI GLQEIKVADE AFPYEITENL GYHVFHHGQK 70 80 90 100 110 120 GHYGVALLTK QEPKVIRRGF PTDNEDAQKR IIMADLETEF GLLTVINGYF PQGESRAHET 130 140 150 160 170 180 KFPAKEKFYA DLQQYLEKEH DKSNPILIMG DMNISPSDLD IGIGDENRKR WLRTGKCSFL 190 200 210 220 230 240 PEERAWYQRL YDYGLEDSFR KLNPTANDKF SWFDYRSKGF DDNRGLRIDH ILVSQKLAER 250 260 CVDVGIALDI RAMEKPSDHA PIWAEFK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21719.1.
PIR	H64044.
RefSeq	NP_438214.1.
3D structure databases
SMR	P44318. Positions 1-267.
ModBase	Search...
Genome annotation databases
GeneID	950938.
GenomeReviews	Gene locus HI0041 in contig L42023_GR.
KEGG	hin:HI0041.
NMPDR	fig|71421.1.peg.40.
TIGR	HI0041.
Phylogenomic databases
HOGENOM	HBG704164.
OMA	NAKRWLK.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0041-MONOMER.
BRENDA	3.1.11.2. 109.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR020847. AP_endonuclease_F1_BS. IPR020848. AP_endonuclease_F1_CS. IPR005135. Endo/exonuclease/phosphatase. IPR004808. exoDNase_III. [Graphical view]
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
TIGRFAMs	TIGR00195. exoDNase_III. 1 hit. TIGR00633. xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EX3_HAEIN

Accession

Primary (citable) accession number: P44318

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)