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Protein

Argininosuccinate synthase

Gene

argG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei100 – 1001CitrullineBy similarity
Binding sitei130 – 1301ATP; via amide nitrogenBy similarity
Binding sitei132 – 1321AspartateBy similarity
Binding sitei132 – 1321ATPBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361CitrullineBy similarity
Binding sitei137 – 1371AspartateBy similarity
Binding sitei137 – 1371ATPBy similarity
Binding sitei140 – 1401CitrullineBy similarity
Binding sitei193 – 1931CitrullineBy similarity
Binding sitei195 – 1951ATPBy similarity
Binding sitei202 – 2021CitrullineBy similarity
Binding sitei204 – 2041CitrullineBy similarity
Binding sitei281 – 2811CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:HI_1727
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Argininosuccinate synthasePRO_0000148700Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1727.

Structurei

3D structure databases

ProteinModelPortaliP44315.
SMRiP44315. Positions 4-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.
PhylomeDBiP44315.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTILQNLP KGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD
60 70 80 90 100
EDDYNAIPKK AMAYGAENAR LIDCRAQLAH EGIAAIQCGA FHISTGGIPY
110 120 130 140 150
FNTTPLGRAV TGTMLVAAMK EDDVNIWGDG STFKGNDIER FYRYGLLTNP
160 170 180 190 200
NLKIYKPWLD VQFIEELGGR LEMSQFLIEN GFDYKMSVEK AYSTDSNMLG
210 220 230 240 250
ATHEAKDLEQ LSTGMKIVKP IMGVAFWDEK VEIKPETVTV TFEDGVPVAL
260 270 280 290 300
NGKHFDNAVD LILEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL
310 320 330 340 350
LHIAYERLVT GIHNEDTIEQ YRINGIRLGR LLYQGRWFDP QALMLRETAQ
360 370 380 390 400
RWVAKAITGT VTLELRRGND FTILNTESPN LTYEAERLSM EKVEDAPFDP
410 420 430 440
IDRIGQLTMR NLDVSDTRGK LGIYAQTGLL SAIKDSVLPQ LGKK
Length:444
Mass (Da):49,289
Last modified:November 1, 1995 - v1
Checksum:iF833EDD718199098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23373.1.
PIRiF64138.
RefSeqiNP_439868.1. NC_000907.1.
WP_005694213.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23373; AAC23373; HI_1727.
GeneIDi950537.
KEGGihin:HI1727.
PATRICi20192207. VBIHaeInf48452_1806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23373.1.
PIRiF64138.
RefSeqiNP_439868.1. NC_000907.1.
WP_005694213.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP44315.
SMRiP44315. Positions 4-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23373; AAC23373; HI_1727.
GeneIDi950537.
KEGGihin:HI1727.
PATRICi20192207. VBIHaeInf48452_1806.

Phylogenomic databases

eggNOGiCOG0137.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.
PhylomeDBiP44315.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiASSY_HAEIN
AccessioniPrimary (citable) accession number: P44315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.