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P44140

- TMCA_HAEIN

UniProt

P44140 - TMCA_HAEIN

Protein

tRNA(Met) cytidine acetyltransferase TmcA

Gene

tmcA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).UniRule annotation

    Catalytic activityi

    (Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451ATPUniRule annotation
    Binding sitei291 – 2911ATPUniRule annotation
    Binding sitei510 – 5101Acetyl-CoAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi167 – 17610ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP
    3. tRNA N-acetyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA acetylation Source: UniProtKB-HAMAP
    2. tRNA wobble cytosine modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA(Met) cytidine acetyltransferase TmcAUniRule annotation (EC:2.3.1.193UniRule annotation)
    Gene namesi
    Name:tmcAUniRule annotation
    Ordered Locus Names:HI_1254/HI_1255/HI_1256
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 656656tRNA(Met) cytidine acetyltransferase TmcAPRO_0000013891Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1254.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini368 – 542175N-acetyltransferaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni474 – 4763Acetyl-CoA bindingUniRule annotation
    Regioni481 – 4877Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the TmcA family.UniRule annotation
    Contains 1 N-acetyltransferase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1444.
    OrthoDBiEOG67T5F2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    3.40.630.30. 2 hits.
    HAMAPiMF_01886. tRNA_acetyltr_TmcA.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR013562. DUF1726.
    IPR000182. GNAT_dom.
    IPR007807. Helicase_dom.
    IPR027417. P-loop_NTPase.
    IPR024914. tRNA_acetyltr_TmcA.
    [Graphical view]
    PfamiPF08351. DUF1726. 1 hit.
    PF13718. GNAT_acetyltr_2. 1 hit.
    PF05127. Helicase_RecD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P44140-1 [UniParc]FASTAAdd to Basket

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    MPSRQLQILI RKTLPLVPDH VLIIGESGIA FSKATNLLGQ EFEHILFDGR    50
    NGIHLEALAI AAGTLKMGGT LCLVLSDWEN LSQQPDQDSL RWNGNQSAIA 100
    TPNFIYHFKQ CIERYHFPIL REESAVEFPT VFYSNEHHKN ATLAQQQIIE 150
    NILQAEQDIY FLTAKRGRGK SALLGMLANQ IQAPVYLTAP NKSAVHSVIE 200
    FSEGDIEFIA PDELALTLQT EPEFSQSAWL LVDEAAMIPL PLLQEYSRYF 250
    QHIVFSTTIH SYEGTGRGFE LKFKRKIHRT FQHFELKQPL RWQENDPLEH 300
    FIDDLLLLNA EDDFQHFDYS NITYNIEENA KNLSFPCLRG KVPEGPKGDL 350
    DIASLPQALE ALLTSKGSEG KYNRQFFFRD FYGLMTIAHY RTSPLDLRRL 400
    LDGKNQRFYF AEYQQNLLGA IWALEEGNMA DDELIIQIQQ GKRRPKGNLV 450
    PQALCFHENL SQACKLRSLR ISRIAVQPNW QQKGIGQNLM QAMENADVDF 500
    LSVSFGYTDE LAKFWQKCGF VLVHLGEHQE ASSGCYSAIA LKGISKEGLA 550
    LVDTAYKQFQ RNLPLSFHPF AINFEQNQLD WQLDDFDWMS LKNFANFHRT 600
    LFSSIPAMRR LLKLAGKENF PLISAYLTKK QFPINKKKGV ECLRLEIKQY 650
    LERGTL 656
    Length:656
    Mass (Da):75,162
    Last modified:January 11, 2011 - v2
    Checksum:i9975F7685CB45303
    GO

    Sequence cautioni

    The sequence AAC22904.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.
    The sequence AAC22910.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.
    The sequence AAC22911.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22904.1. Frameshift.
    L42023 Genomic DNA. Translation: AAC22910.1. Frameshift.
    L42023 Genomic DNA. Translation: AAC22911.1. Frameshift.
    PIRiE64023.
    F64023.
    G64023.

    Genome annotation databases

    EnsemblBacteriaiAAC22904; AAC22904; HI_1254.
    AAC22910; AAC22910; HI_1255.
    AAC22911; AAC22911; HI_1256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22904.1 . Frameshift.
    L42023 Genomic DNA. Translation: AAC22910.1 . Frameshift.
    L42023 Genomic DNA. Translation: AAC22911.1 . Frameshift.
    PIRi E64023.
    F64023.
    G64023.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22904 ; AAC22904 ; HI_1254 .
    AAC22910 ; AAC22910 ; HI_1255 .
    AAC22911 ; AAC22911 ; HI_1256 .

    Phylogenomic databases

    eggNOGi COG1444.
    OrthoDBi EOG67T5F2.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    3.40.630.30. 2 hits.
    HAMAPi MF_01886. tRNA_acetyltr_TmcA.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR013562. DUF1726.
    IPR000182. GNAT_dom.
    IPR007807. Helicase_dom.
    IPR027417. P-loop_NTPase.
    IPR024914. tRNA_acetyltr_TmcA.
    [Graphical view ]
    Pfami PF08351. DUF1726. 1 hit.
    PF13718. GNAT_acetyltr_2. 1 hit.
    PF05127. Helicase_RecD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF55729. SSF55729. 1 hit.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiTMCA_HAEIN
    AccessioniPrimary (citable) accession number: P44140
    Secondary accession number(s): P44141, P44142
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Could be the product of a pseudogene. Three frameshifts produce three separate ORFs.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3