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P44140

- TMCA_HAEIN

UniProt

P44140 - TMCA_HAEIN

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Protein
tRNA(Met) cytidine acetyltransferase TmcA
Gene
tmcA, HI_1254/HI_1255/HI_1256
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Protein uncertaini

Functioni

Catalyzes the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) By similarity.UniRule annotation

Catalytic activityi

(Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451ATP By similarity
Binding sitei291 – 2911ATP By similarity
Binding sitei510 – 5101Acetyl-CoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi167 – 17610ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tRNA N-acetyltransferase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA acetylation Source: UniProtKB-HAMAP
  2. tRNA wobble cytosine modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Met) cytidine acetyltransferase TmcA (EC:2.3.1.193)
Gene namesi
Name:tmcA
Ordered Locus Names:HI_1254/HI_1255/HI_1256
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656tRNA(Met) cytidine acetyltransferase TmcAUniRule annotation
PRO_0000013891Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1254.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini368 – 542175N-acetyltransferase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni474 – 4763Acetyl-CoA binding By similarity
Regioni481 – 4877Acetyl-CoA binding By similarity

Sequence similaritiesi

Belongs to the TmcA family.

Phylogenomic databases

eggNOGiCOG1444.
OrthoDBiEOG67T5F2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.630.30. 2 hits.
HAMAPiMF_01886. tRNA_acetyltr_TmcA.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR013562. DUF1726.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR027417. P-loop_NTPase.
IPR024914. tRNA_acetyltr_TmcA.
[Graphical view]
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44140-1 [UniParc]FASTAAdd to Basket

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MPSRQLQILI RKTLPLVPDH VLIIGESGIA FSKATNLLGQ EFEHILFDGR    50
NGIHLEALAI AAGTLKMGGT LCLVLSDWEN LSQQPDQDSL RWNGNQSAIA 100
TPNFIYHFKQ CIERYHFPIL REESAVEFPT VFYSNEHHKN ATLAQQQIIE 150
NILQAEQDIY FLTAKRGRGK SALLGMLANQ IQAPVYLTAP NKSAVHSVIE 200
FSEGDIEFIA PDELALTLQT EPEFSQSAWL LVDEAAMIPL PLLQEYSRYF 250
QHIVFSTTIH SYEGTGRGFE LKFKRKIHRT FQHFELKQPL RWQENDPLEH 300
FIDDLLLLNA EDDFQHFDYS NITYNIEENA KNLSFPCLRG KVPEGPKGDL 350
DIASLPQALE ALLTSKGSEG KYNRQFFFRD FYGLMTIAHY RTSPLDLRRL 400
LDGKNQRFYF AEYQQNLLGA IWALEEGNMA DDELIIQIQQ GKRRPKGNLV 450
PQALCFHENL SQACKLRSLR ISRIAVQPNW QQKGIGQNLM QAMENADVDF 500
LSVSFGYTDE LAKFWQKCGF VLVHLGEHQE ASSGCYSAIA LKGISKEGLA 550
LVDTAYKQFQ RNLPLSFHPF AINFEQNQLD WQLDDFDWMS LKNFANFHRT 600
LFSSIPAMRR LLKLAGKENF PLISAYLTKK QFPINKKKGV ECLRLEIKQY 650
LERGTL 656
Length:656
Mass (Da):75,162
Last modified:January 11, 2011 - v2
Checksum:i9975F7685CB45303
GO

Sequence cautioni

The sequence AAC22904.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.
The sequence AAC22910.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.
The sequence AAC22911.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22904.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22910.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22911.1. Frameshift.
PIRiE64023.
F64023.
G64023.

Genome annotation databases

EnsemblBacteriaiAAC22904; AAC22904; HI_1254.
AAC22910; AAC22910; HI_1255.
AAC22911; AAC22911; HI_1256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22904.1 . Frameshift.
L42023 Genomic DNA. Translation: AAC22910.1 . Frameshift.
L42023 Genomic DNA. Translation: AAC22911.1 . Frameshift.
PIRi E64023.
F64023.
G64023.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1254.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22904 ; AAC22904 ; HI_1254 .
AAC22910 ; AAC22910 ; HI_1255 .
AAC22911 ; AAC22911 ; HI_1256 .

Phylogenomic databases

eggNOGi COG1444.
OrthoDBi EOG67T5F2.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
3.40.630.30. 2 hits.
HAMAPi MF_01886. tRNA_acetyltr_TmcA.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR013562. DUF1726.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR027417. P-loop_NTPase.
IPR024914. tRNA_acetyltr_TmcA.
[Graphical view ]
Pfami PF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiTMCA_HAEIN
AccessioniPrimary (citable) accession number: P44140
Secondary accession number(s): P44141, P44142
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene. Three frameshifts produce three separate ORFs.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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