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P44140

- TMCA_HAEIN

UniProt

P44140 - TMCA_HAEIN

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Protein

tRNA(Met) cytidine acetyltransferase TmcA

Gene

tmcA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini

Functioni

Catalyzes the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).UniRule annotation

Catalytic activityi

(Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451ATPUniRule annotation
Binding sitei291 – 2911ATPUniRule annotation
Binding sitei510 – 5101Acetyl-CoAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi167 – 17610ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
  3. tRNA N-acetyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA acetylation Source: UniProtKB-HAMAP
  2. tRNA wobble cytosine modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Met) cytidine acetyltransferase TmcAUniRule annotation (EC:2.3.1.193UniRule annotation)
Gene namesi
Name:tmcAUniRule annotation
Ordered Locus Names:HI_1254/HI_1255/HI_1256
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656tRNA(Met) cytidine acetyltransferase TmcAPRO_0000013891Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1254.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini368 – 542175N-acetyltransferaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni474 – 4763Acetyl-CoA bindingUniRule annotation
Regioni481 – 4877Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

Belongs to the TmcA family.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1444.
OrthoDBiEOG67T5F2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.630.30. 2 hits.
HAMAPiMF_01886. tRNA_acetyltr_TmcA.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR013562. DUF1726.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR027417. P-loop_NTPase.
IPR024914. tRNA_acetyltr_TmcA.
[Graphical view]
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44140-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MPSRQLQILI RKTLPLVPDH VLIIGESGIA FSKATNLLGQ EFEHILFDGR
60 70 80 90 100
NGIHLEALAI AAGTLKMGGT LCLVLSDWEN LSQQPDQDSL RWNGNQSAIA
110 120 130 140 150
TPNFIYHFKQ CIERYHFPIL REESAVEFPT VFYSNEHHKN ATLAQQQIIE
160 170 180 190 200
NILQAEQDIY FLTAKRGRGK SALLGMLANQ IQAPVYLTAP NKSAVHSVIE
210 220 230 240 250
FSEGDIEFIA PDELALTLQT EPEFSQSAWL LVDEAAMIPL PLLQEYSRYF
260 270 280 290 300
QHIVFSTTIH SYEGTGRGFE LKFKRKIHRT FQHFELKQPL RWQENDPLEH
310 320 330 340 350
FIDDLLLLNA EDDFQHFDYS NITYNIEENA KNLSFPCLRG KVPEGPKGDL
360 370 380 390 400
DIASLPQALE ALLTSKGSEG KYNRQFFFRD FYGLMTIAHY RTSPLDLRRL
410 420 430 440 450
LDGKNQRFYF AEYQQNLLGA IWALEEGNMA DDELIIQIQQ GKRRPKGNLV
460 470 480 490 500
PQALCFHENL SQACKLRSLR ISRIAVQPNW QQKGIGQNLM QAMENADVDF
510 520 530 540 550
LSVSFGYTDE LAKFWQKCGF VLVHLGEHQE ASSGCYSAIA LKGISKEGLA
560 570 580 590 600
LVDTAYKQFQ RNLPLSFHPF AINFEQNQLD WQLDDFDWMS LKNFANFHRT
610 620 630 640 650
LFSSIPAMRR LLKLAGKENF PLISAYLTKK QFPINKKKGV ECLRLEIKQY

LERGTL
Length:656
Mass (Da):75,162
Last modified:January 11, 2011 - v2
Checksum:i9975F7685CB45303
GO

Sequence cautioni

The sequence AAC22904.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502. Curated
The sequence AAC22910.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502. Curated
The sequence AAC22911.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22904.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22910.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22911.1. Frameshift.
PIRiE64023.
F64023.
G64023.

Genome annotation databases

EnsemblBacteriaiAAC22904; AAC22904; HI_1254.
AAC22910; AAC22910; HI_1255.
AAC22911; AAC22911; HI_1256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22904.1 . Frameshift.
L42023 Genomic DNA. Translation: AAC22910.1 . Frameshift.
L42023 Genomic DNA. Translation: AAC22911.1 . Frameshift.
PIRi E64023.
F64023.
G64023.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1254.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22904 ; AAC22904 ; HI_1254 .
AAC22910 ; AAC22910 ; HI_1255 .
AAC22911 ; AAC22911 ; HI_1256 .

Phylogenomic databases

eggNOGi COG1444.
OrthoDBi EOG67T5F2.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
3.40.630.30. 2 hits.
HAMAPi MF_01886. tRNA_acetyltr_TmcA.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR013562. DUF1726.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR027417. P-loop_NTPase.
IPR024914. tRNA_acetyltr_TmcA.
[Graphical view ]
Pfami PF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiTMCA_HAEIN
AccessioniPrimary (citable) accession number: P44140
Secondary accession number(s): P44141, P44142
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2011
Last modified: October 29, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene. Three frameshifts produce three separate ORFs.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3