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P44140 (TMCA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA(Met) cytidine acetyltransferase TmcA

EC=2.3.1.193
Gene names
Name:tmcA
Ordered Locus Names:HI_1254/HI_1255/HI_1256
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceUncertain

General annotation (Comments)

Function

Catalyzes the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) By similarity. HAMAP-Rule MF_01886

Catalytic activity

(Elongator tRNA(Met))-cytidine34 + ATP + acetyl-CoA = (elongator tRNA(Met))-N(4)-acetylcytidine34 + ADP + phosphate + CoA. HAMAP-Rule MF_01886

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01886.

Sequence similarities

Belongs to the TmcA family.

Contains 1 N-acetyltransferase domain.

Caution

Could be the product of a pseudogene. Three frameshifts produce three separate ORFs.

Sequence caution

The sequence AAC22904.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.

The sequence AAC22910.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.

The sequence AAC22911.1 differs from that shown. Reason: Frameshift at positions 349, 389 and 502.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA acetylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA wobble cytosine modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA N-acetyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 656656tRNA(Met) cytidine acetyltransferase TmcA HAMAP-Rule MF_01886
PRO_0000013891

Regions

Domain368 – 542175N-acetyltransferase
Nucleotide binding167 – 17610ATP By similarity
Region474 – 4763Acetyl-CoA binding By similarity
Region481 – 4877Acetyl-CoA binding By similarity

Sites

Binding site1451ATP By similarity
Binding site2911ATP By similarity
Binding site5101Acetyl-CoA By similarity

Sequences

Sequence LengthMass (Da)Tools
P44140 [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: 9975F7685CB45303

FASTA65675,162
        10         20         30         40         50         60 
MPSRQLQILI RKTLPLVPDH VLIIGESGIA FSKATNLLGQ EFEHILFDGR NGIHLEALAI 

        70         80         90        100        110        120 
AAGTLKMGGT LCLVLSDWEN LSQQPDQDSL RWNGNQSAIA TPNFIYHFKQ CIERYHFPIL 

       130        140        150        160        170        180 
REESAVEFPT VFYSNEHHKN ATLAQQQIIE NILQAEQDIY FLTAKRGRGK SALLGMLANQ 

       190        200        210        220        230        240 
IQAPVYLTAP NKSAVHSVIE FSEGDIEFIA PDELALTLQT EPEFSQSAWL LVDEAAMIPL 

       250        260        270        280        290        300 
PLLQEYSRYF QHIVFSTTIH SYEGTGRGFE LKFKRKIHRT FQHFELKQPL RWQENDPLEH 

       310        320        330        340        350        360 
FIDDLLLLNA EDDFQHFDYS NITYNIEENA KNLSFPCLRG KVPEGPKGDL DIASLPQALE 

       370        380        390        400        410        420 
ALLTSKGSEG KYNRQFFFRD FYGLMTIAHY RTSPLDLRRL LDGKNQRFYF AEYQQNLLGA 

       430        440        450        460        470        480 
IWALEEGNMA DDELIIQIQQ GKRRPKGNLV PQALCFHENL SQACKLRSLR ISRIAVQPNW 

       490        500        510        520        530        540 
QQKGIGQNLM QAMENADVDF LSVSFGYTDE LAKFWQKCGF VLVHLGEHQE ASSGCYSAIA 

       550        560        570        580        590        600 
LKGISKEGLA LVDTAYKQFQ RNLPLSFHPF AINFEQNQLD WQLDDFDWMS LKNFANFHRT 

       610        620        630        640        650 
LFSSIPAMRR LLKLAGKENF PLISAYLTKK QFPINKKKGV ECLRLEIKQY LERGTL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22904.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22910.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22911.1. Frameshift.
PIRE64023.
F64023.
G64023.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22904; AAC22904; HI_1254.
AAC22910; AAC22910; HI_1255.
AAC22911; AAC22911; HI_1256.

Phylogenomic databases

eggNOGCOG1444.
OrthoDBEOG67T5F2.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
3.40.630.30. 2 hits.
HAMAPMF_01886. tRNA_acetyltr_TmcA.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR013562. DUF1726.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR027417. P-loop_NTPase.
IPR024914. tRNA_acetyltr_TmcA.
[Graphical view]
PfamPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTMCA_HAEIN
AccessionPrimary (citable) accession number: P44140
Secondary accession number(s): P44141, P44142
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names