Reviewed, UniProtKB/Swiss-Prot P44121 (DNLI_HAEIN)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	ligA
Ordered Locus Names:	HI1182/HI1183

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes efficient strand joining on a single nicked DNA.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.
Caution	Was originally (Ref.2) proposed to encode for two separate adjacent ORFs, HI1182 and HI1183.
Sequence caution	The sequence AAC22836.1 differs from that shown. Reason: Frameshift at position 183. The sequence AAC22846.1 differs from that shown. Reason: Frameshift at position 183.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	DNA ligase		PRO_0000059625
Sites
	Active site	41	1	N6-AMP-lysine intermediate Potential
Experimental info
	Sequence conflict	93	1	T → S in AAC22836. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P44121-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: F9EF9DD95B6B1252	FASTA	268	30,896
	10 20 30 40 50 60 MKFYRTLLLF FASSFAFANS DLMLLHTYNN QPIEGWVMSE KLDGVRGYWN GKQLLTRQGQ 70 80 90 100 110 120 RLSPPAYFIK DFPPFAIDGE LFSERNHFEE ISTITKSFKG DGWEKLKLYV FDVPDAEGNL 130 140 150 160 170 180 FERLAKLKAH LLEHPTTYIE IIEQIPVKDK THLYQFLAQV ENLQGEGVVV RNPNAPYERK 190 200 210 220 230 240 RSSQILKLKT ARGEECTVIA HHKGKGQFEN VMGALTCKNH RGEFKIGSGF NLNERENPPP 250 260 IGSVITYKYR GITNSGKPRF ATYWREKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Altered lipopolysaccharide characteristic of the I69 phenotype in Haemophilus influenzae results from mutations in a novel gene, isn." Preston A., Maskell D., Johnson A., Moxon E.R. J. Bacteriol. 178:396-402(1996) [PubMed: 8550458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[3]	"Characterization of an ATP-dependent DNA ligase encoded by Haemophilus influenzae." Cheng C., Shuman S. Nucleic Acids Res. 25:1369-1374(1997) [PubMed: 9060431] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U17295 Genomic DNA. Translation: AAA95982.1. L42023 Genomic DNA. Translation: AAC22836.1. Frameshift. L42023 Genomic DNA. Translation: AAC22846.1. Frameshift.
PIR	D64021. E64021.
3D structure databases
ModBase	Search...
Genome annotation databases
TIGR	HI1182. HI1183.
Phylogenomic databases
HOGENOM	P44121.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1182-MON. HINF71421:HI_1183-MON.
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PROSITE	PS00697. DNA_LIGASE_A1. False negative. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_HAEIN

Accession

Primary (citable) accession number: P44121
Secondary accession number(s): P44122, P71371, P71372

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)