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P44009 (APHA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Class B acid phosphatase

Short name=CBAP
EC=3.1.3.2
Gene names
Name:aphA
Synonyms:napA
Ordered Locus Names:HI_0494/HI_0495
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class B bacterial acid phosphatase family.

Sequence caution

The sequence AAC22151.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.

The sequence AAC22152.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 236214Class B acid phosphatase
PRO_0000024005

Regions

Region136 – 1372Substrate binding By similarity

Sites

Active site671Nucleophile By similarity
Active site691Proton donor By similarity
Metal binding671Magnesium By similarity
Metal binding691Magnesium; via carbonyl oxygen By similarity
Metal binding1911Magnesium By similarity
Binding site1761Substrate By similarity

Experimental info

Sequence conflict731Missing in CAA68889. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P44009 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D5D7091AC6BB4418

FASTA23626,271
        10         20         30         40         50         60 
MKNVMKLSVI ALLTAAAVPA MAGKTEPYTQ SGTNAREMLQ EQAIHWISVD QIKQSLEGKA 

        70         80         90        100        110        120 
PINVSFDIDD TVMLFSSPCF YHGQQKFSPG KHDYLKNQDF WNEVNAGCDK YSIPKQIAID 

       130        140        150        160        170        180 
LINMHQARGD QVYFFTGRTA GKVDGVTPIL EKTFNIKNMH PVEFMGSRER TTKYNKTPAI 

       190        200        210        220        230 
ISHKVSIHYG DSDDDVLAAK EAGVRGIRLM RAANSTYQPM PTLGGYGEEV LINSSY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22151.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22152.1. Frameshift.
Y07615 Genomic DNA. Translation: CAA68889.1.
PIRT09434.
RefSeqNP_438653.1. NC_000907.1.

3D structure databases

ProteinModelPortalP44009.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0495m.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22151; AAC22151; HI_0494.
AAC22152; AAC22152; HI_0495.
GeneID950561.
KEGGhin:HI0495m.
PATRIC20189539. VBIHaeInf48452_0512.

Phylogenomic databases

eggNOGCOG3700.
KOK03788.
OrthoDBEOG6HTNVZ.
ProtClustDBPRK11009.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01672. AphA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAPHA_HAEIN
AccessionPrimary (citable) accession number: P44009
Secondary accession number(s): P44730, P77869
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names