P44009 (APHA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
Short name=CBAP
EC=3.1.3.2

Gene names

Name:	aphA
Synonyms:	napA
Ordered Locus Names:	HI_0494/HI_0495

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.
Sequence caution	The sequence AAC22151.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs. The sequence AAC22152.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 236	214	Class B acid phosphatase		PRO_0000024005
Regions
	Region	136 – 137	2	Substrate binding By similarity
Sites
	Active site	67	1	Nucleophile By similarity
	Active site	69	1	Proton donor By similarity
	Metal binding	67	1	Magnesium By similarity
	Metal binding	69	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	191	1	Magnesium By similarity
	Binding site	176	1	Substrate By similarity
Experimental info
	Sequence conflict	73	1	Missing in CAA68889. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P44009 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D5D7091AC6BB4418	FASTA	236	26,271
	10 20 30 40 50 60 MKNVMKLSVI ALLTAAAVPA MAGKTEPYTQ SGTNAREMLQ EQAIHWISVD QIKQSLEGKA 70 80 90 100 110 120 PINVSFDIDD TVMLFSSPCF YHGQQKFSPG KHDYLKNQDF WNEVNAGCDK YSIPKQIAID 130 140 150 160 170 180 LINMHQARGD QVYFFTGRTA GKVDGVTPIL EKTFNIKNMH PVEFMGSRER TTKYNKTPAI 190 200 210 220 230 ISHKVSIHYG DSDDDVLAAK EAGVRGIRLM RAANSTYQPM PTLGGYGEEV LINSSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	Rossolini G.M., Bonci A., Schippa S., Iori P., Thaller M.C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCUG 7317/A.
[3]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22151.1. Frameshift. L42023 Genomic DNA. Translation: AAC22152.1. Frameshift. Y07615 Genomic DNA. Translation: CAA68889.1.
PIR	T09434.
RefSeq	NP_438653.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44009.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0495m.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22151; AAC22151; HI_0494. AAC22152; AAC22152; HI_0495.
GeneID	950561.
KEGG	hin:HI0495m.
PATRIC	20189539. VBIHaeInf48452_0512.
Phylogenomic databases
eggNOG	COG3700.
KO	K03788.
ProtClustDB	PRK11009.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR005519. Acid_phosphat_B. IPR023214. HAD-like_dom. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_HAEIN

Accession

Primary (citable) accession number: P44009
Secondary accession number(s): P44730, P77869

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program