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P44009

- APHA_HAEIN

UniProt

P44009 - APHA_HAEIN

Protein

Class B acid phosphatase

Gene

aphA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.By similarity

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671NucleophileBy similarity
    Metal bindingi67 – 671MagnesiumBy similarity
    Active sitei69 – 691Proton donorBy similarity
    Metal bindingi69 – 691Magnesium; via carbonyl oxygenBy similarity
    Binding sitei176 – 1761SubstrateBy similarity
    Metal bindingi191 – 1911MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphatase (EC:3.1.3.2)
    Short name:
    CBAP
    Gene namesi
    Name:aphA
    Synonyms:napA
    Ordered Locus Names:HI_0494/HI_0495
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 236214Class B acid phosphatasePRO_0000024005Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi71421.HI0495m.

    Structurei

    3D structure databases

    ProteinModelPortaliP44009.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 1372Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3700.
    KOiK03788.
    OrthoDBiEOG6HTNVZ.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P44009-1 [UniParc]FASTAAdd to Basket

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    MKNVMKLSVI ALLTAAAVPA MAGKTEPYTQ SGTNAREMLQ EQAIHWISVD    50
    QIKQSLEGKA PINVSFDIDD TVMLFSSPCF YHGQQKFSPG KHDYLKNQDF 100
    WNEVNAGCDK YSIPKQIAID LINMHQARGD QVYFFTGRTA GKVDGVTPIL 150
    EKTFNIKNMH PVEFMGSRER TTKYNKTPAI ISHKVSIHYG DSDDDVLAAK 200
    EAGVRGIRLM RAANSTYQPM PTLGGYGEEV LINSSY 236
    Length:236
    Mass (Da):26,271
    Last modified:November 1, 1995 - v1
    Checksum:iD5D7091AC6BB4418
    GO

    Sequence cautioni

    The sequence AAC22151.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.
    The sequence AAC22152.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731Missing in CAA68889. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22151.1. Frameshift.
    L42023 Genomic DNA. Translation: AAC22152.1. Frameshift.
    Y07615 Genomic DNA. Translation: CAA68889.1.
    PIRiT09434.
    RefSeqiNP_438653.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22151; AAC22151; HI_0494.
    AAC22152; AAC22152; HI_0495.
    GeneIDi950561.
    KEGGihin:HI0495m.
    PATRICi20189539. VBIHaeInf48452_0512.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22151.1 . Frameshift.
    L42023 Genomic DNA. Translation: AAC22152.1 . Frameshift.
    Y07615 Genomic DNA. Translation: CAA68889.1 .
    PIRi T09434.
    RefSeqi NP_438653.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44009.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0495m.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22151 ; AAC22151 ; HI_0494 .
    AAC22152 ; AAC22152 ; HI_0495 .
    GeneIDi 950561.
    KEGGi hin:HI0495m.
    PATRICi 20189539. VBIHaeInf48452_0512.

    Phylogenomic databases

    eggNOGi COG3700.
    KOi K03788.
    OrthoDBi EOG6HTNVZ.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. Rossolini G.M., Bonci A., Schippa S., Iori P., Thaller M.C.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CCUG 7317/A.
    3. "Two-dimensional map of the proteome of Haemophilus influenzae."
      Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
      Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiAPHA_HAEIN
    AccessioniPrimary (citable) accession number: P44009
    Secondary accession number(s): P44730, P77869
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3