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Protein

Class B acid phosphatase

Gene

aphA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67NucleophileBy similarity1
Metal bindingi67MagnesiumBy similarity1
Active sitei69Proton donorBy similarity1
Metal bindingi69Magnesium; via carbonyl oxygenBy similarity1
Binding sitei176SubstrateBy similarity1
Metal bindingi191MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2By similarity)
Short name:
CBAP
Gene namesi
Name:aphA
Synonyms:napA
Ordered Locus Names:HI_0494/HI_0495
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002400523 – 236Class B acid phosphataseAdd BLAST214

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0495m.

Structurei

3D structure databases

ProteinModelPortaliP44009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 137Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
KOiK03788.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVMKLSVI ALLTAAAVPA MAGKTEPYTQ SGTNAREMLQ EQAIHWISVD
60 70 80 90 100
QIKQSLEGKA PINVSFDIDD TVMLFSSPCF YHGQQKFSPG KHDYLKNQDF
110 120 130 140 150
WNEVNAGCDK YSIPKQIAID LINMHQARGD QVYFFTGRTA GKVDGVTPIL
160 170 180 190 200
EKTFNIKNMH PVEFMGSRER TTKYNKTPAI ISHKVSIHYG DSDDDVLAAK
210 220 230
EAGVRGIRLM RAANSTYQPM PTLGGYGEEV LINSSY
Length:236
Mass (Da):26,271
Last modified:November 1, 1995 - v1
Checksum:iD5D7091AC6BB4418
GO

Sequence cautioni

The sequence AAC22151 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.Curated
The sequence AAC22152 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73Missing in CAA68889 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22151.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22152.1. Frameshift.
Y07615 Genomic DNA. Translation: CAA68889.1.
PIRiT09434.
RefSeqiNP_438653.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22151; AAC22151; HI_0494.
AAC22152; AAC22152; HI_0495.
GeneIDi950561.
KEGGihin:HI0495m.
PATRICi20189539. VBIHaeInf48452_0512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22151.1. Frameshift.
L42023 Genomic DNA. Translation: AAC22152.1. Frameshift.
Y07615 Genomic DNA. Translation: CAA68889.1.
PIRiT09434.
RefSeqiNP_438653.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP44009.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0495m.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22151; AAC22151; HI_0494.
AAC22152; AAC22152; HI_0495.
GeneIDi950561.
KEGGihin:HI0495m.
PATRICi20189539. VBIHaeInf48452_0512.

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.
KOiK03788.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_HAEIN
AccessioniPrimary (citable) accession number: P44009
Secondary accession number(s): P44730, P77869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.