Class B acid phosphatase precursor - Haemophilus influenzae

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Reviewed, UniProtKB/Swiss-Prot P44009 (APHA_HAEIN)

Last modified June 16, 2009. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
EC=3.1.3.2

Gene names

Name:	aphA
Synonyms:	napA
Ordered Locus Names:	HI0494/HI0495

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.
Sequence caution	The sequence AAC22152.1 differs from that shown. Reason: Frameshift at position 66. Produces two separate ORFs.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

22

Potential

Chain

214

Class B acid phosphatase

PRO_0000024005

Sites

Metal binding

1

Magnesium By similarity

Metal binding

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

1

Magnesium By similarity

Experimental info

Sequence conflict

1

Missing in CAA68889. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P44009-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D5D7091AC6BB4418
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236

26,271

        10         20         30         40         50         60 
MKNVMKLSVI ALLTAAAVPA MAGKTEPYTQ SGTNAREMLQ EQAIHWISVD QIKQSLEGKA 

        70         80         90        100        110        120 
PINVSFDIDD TVMLFSSPCF YHGQQKFSPG KHDYLKNQDF WNEVNAGCDK YSIPKQIAID 

       130        140        150        160        170        180 
LINMHQARGD QVYFFTGRTA GKVDGVTPIL EKTFNIKNMH PVEFMGSRER TTKYNKTPAI 

       190        200        210        220        230 
ISHKVSIHYG DSDDDVLAAK EAGVRGIRLM RAANSTYQPM PTLGGYGEEV LINSSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	Rossolini G.M., Bonci A., Schippa S., Iori P., Thaller M.C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCUG 7317/A.
[3]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed: 10675023] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
	L42023 Genomic DNA. Translation: AAC22151.1. Frameshift. L42023 Genomic DNA. Translation: AAC22152.1. Frameshift. Y07615 Genomic DNA. Translation: CAA68889.1.
PIR	T09434.
RefSeq	NP_438653.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950561.
TIGR	HI0494. HI0495.
Phylogenomic databases
HOGENOM	P44009.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0494-MON. HINF71421:HI_0495-MON.
BRENDA	3.1.3.2. 109.
Family and domain databases
InterPro	IPR005519. Acid_phosphat_B. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_HAEIN

Accession

Primary (citable) accession number: P44009
Secondary accession number(s): P44730, P77869

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents