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Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

Catalytic activityi

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Iron1
Metal bindingi58Iron1
Metal bindingi128Iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Autoinducer synthesis, Quorum sensing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi4.4.1.21. 2529.

Names & Taxonomyi

Protein namesi
Recommended name:
S-ribosylhomocysteine lyase (EC:4.4.1.21)
Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS
Gene namesi
Name:luxS
Ordered Locus Names:HI_0491
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001722272 – 167S-ribosylhomocysteine lyaseAdd BLAST166

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi71421.HI0491.

Structurei

Secondary structure

1167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Helixi11 – 13Combined sources3
Beta strandi16 – 26Combined sources11
Beta strandi32 – 39Combined sources8
Turni43 – 45Combined sources3
Helixi50 – 68Combined sources19
Beta strandi73 – 80Combined sources8
Beta strandi84 – 93Combined sources10
Helixi97 – 112Combined sources16
Helixi117 – 119Combined sources3
Turni125 – 127Combined sources3
Turni129 – 132Combined sources4
Helixi136 – 149Combined sources14
Beta strandi152 – 154Combined sources3
Helixi156 – 159Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J6WX-ray2.10A/B2-167[»]
1JOEX-ray2.40A/B/C/D1-167[»]
ProteinModelPortaliP44007.
SMRiP44007.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44007.

Family & Domainsi

Sequence similaritiesi

Belongs to the LuxS family.Curated

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
KOiK07173.
OMAiAGFMREH.
PhylomeDBiP44007.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamiPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF63411. SSF63411. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLDSFKVD HTKMNAPAVR IAKTMLTPKG DNITVFDLRF CIPNKEILSP
60 70 80 90 100
KGIHTLEHLF AGFMRDHLNG DSIEIIDISP MGCRTGFYMS LIGTPNEQKV
110 120 130 140 150
SEAWLASMQD VLGVQDQASI PELNIYQCGS YTEHSLEDAH EIAKNVIARG
160
IGVNKNEDLS LDNSLLK
Length:167
Mass (Da):18,527
Last modified:January 23, 2007 - v3
Checksum:iA8116FE96BA5F817
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22149.1.
PIRiG64008.
RefSeqiNP_438651.1. NC_000907.1.
WP_005693682.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22149; AAC22149; HI_0491.
GeneIDi950775.
KEGGihin:HI0491.
PATRICi20189535. VBIHaeInf48452_0510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22149.1.
PIRiG64008.
RefSeqiNP_438651.1. NC_000907.1.
WP_005693682.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J6WX-ray2.10A/B2-167[»]
1JOEX-ray2.40A/B/C/D1-167[»]
ProteinModelPortaliP44007.
SMRiP44007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22149; AAC22149; HI_0491.
GeneIDi950775.
KEGGihin:HI0491.
PATRICi20189535. VBIHaeInf48452_0510.

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
KOiK07173.
OMAiAGFMREH.
PhylomeDBiP44007.

Enzyme and pathway databases

BRENDAi4.4.1.21. 2529.

Miscellaneous databases

EvolutionaryTraceiP44007.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
[Graphical view]
PfamiPF02664. LuxS. 1 hit.
[Graphical view]
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiPD013172. S-ribosylhomocysteinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF63411. SSF63411. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLUXS_HAEIN
AccessioniPrimary (citable) accession number: P44007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.