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P43985

- CMOA_HAEIN

UniProt

P43985 - CMOA_HAEIN

Protein

tRNA (cmo5U34)-methyltransferase

Gene

cmoA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei38 – 381S-adenosyl-L-methionine
    Binding sitei88 – 881S-adenosyl-L-methionine
    Binding sitei131 – 1311S-adenosyl-L-methionine
    Binding sitei198 – 1981SubstrateSequence Analysis

    GO - Molecular functioni

    1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA (uracil) methyltransferase activity Source: InterPro

    GO - Biological processi

    1. tRNA wobble uridine modification Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (cmo5U34)-methyltransferase (EC:2.1.1.-)
    Gene namesi
    Name:cmoA
    Ordered Locus Names:HI_0319
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 241241tRNA (cmo5U34)-methyltransferasePRO_0000169084Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi71421.HI0319.

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi21 – 3414
    Helixi38 – 5215
    Beta strandi58 – 636
    Helixi68 – 758
    Beta strandi83 – 875
    Helixi91 – 10212
    Beta strandi110 – 1134
    Turni117 – 1193
    Beta strandi124 – 1329
    Helixi134 – 1363
    Helixi139 – 1413
    Helixi142 – 15211
    Beta strandi153 – 16412
    Helixi170 – 18617
    Helixi189 – 1913
    Helixi195 – 20410
    Helixi210 – 22011
    Beta strandi223 – 2319
    Beta strandi234 – 2407

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IM8X-ray2.20A/B1-241[»]
    ProteinModelPortaliP43985.
    SMRiP43985. Positions 17-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43985.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 653S-adenosyl-L-methionine binding
    Regioni116 – 1172S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0500.
    KOiK15256.
    OMAiYDLGCSR.
    OrthoDBiEOG628F44.
    PhylomeDBiP43985.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01589. tRNA_methyltr_CmoA.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR005271. tRNA_cmo5U34_MeTrfase.
    [Graphical view]
    PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43985-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKDTLFSTP IAKLGDFIFD ENVAEVFPDM IQRSVPGYSN IITAIGMLAE    50
    RFVTADSNVY DLGCSRGAAT LSARRNINQP NVKIIGIDNS QPMVERCRQH 100
    IAAYHSEIPV EILCNDIRHV EIKNASMVIL NFTLQFLPPE DRIALLTKIY 150
    EGLNPNGVLV LSEKFRFEDT KINHLLIDLH HQFKRANGYS ELEVSQKRTA 200
    LENVMRTDSI ETHKVRLKNV GFSQVELWFQ CFNFGSMIAV K 241
    Length:241
    Mass (Da):27,372
    Last modified:November 1, 1995 - v1
    Checksum:i08743633A3E853F8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21983.1.
    PIRiC64006.
    RefSeqiNP_438485.1. NC_000907.1.
    WP_005694346.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC21983; AAC21983; HI_0319.
    GeneIDi949432.
    KEGGihin:HI0319.
    PATRICi20189181. VBIHaeInf48452_0337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21983.1 .
    PIRi C64006.
    RefSeqi NP_438485.1. NC_000907.1.
    WP_005694346.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IM8 X-ray 2.20 A/B 1-241 [» ]
    ProteinModelPortali P43985.
    SMRi P43985. Positions 17-241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC21983 ; AAC21983 ; HI_0319 .
    GeneIDi 949432.
    KEGGi hin:HI0319.
    PATRICi 20189181. VBIHaeInf48452_0337.

    Phylogenomic databases

    eggNOGi COG0500.
    KOi K15256.
    OMAi YDLGCSR.
    OrthoDBi EOG628F44.
    PhylomeDBi P43985.

    Miscellaneous databases

    EvolutionaryTracei P43985.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01589. tRNA_methyltr_CmoA.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR005271. tRNA_cmo5U34_MeTrfase.
    [Graphical view ]
    PIRSFi PIRSF006325. MeTrfase_bac. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00740. TIGR00740. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine."
      Lim K., Zhang H., Tempczyk A., Bonander N., Toedt J., Howard A., Eisenstein E., Herzberg O.
      Proteins 45:397-407(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE, FUNCTION, SUBUNIT.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiCMOA_HAEIN
    AccessioniPrimary (citable) accession number: P43985
    Secondary accession number(s): P43986
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3