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P43985 (CMOA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (cmo5U34)-methyltransferase

EC=2.1.1.-
Gene names
Name:cmoA
Ordered Locus Names:HI_0319
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity. Ref.3

Subunit structure

Monomer. Ref.3

Sequence similarities

Belongs to the methyltransferase superfamily. CmoA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241tRNA (cmo5U34)-methyltransferase HAMAP-Rule MF_01589
PRO_0000169084

Regions

Region63 – 653S-adenosyl-L-methionine binding HAMAP-Rule MF_01589
Region116 – 1172S-adenosyl-L-methionine binding HAMAP-Rule MF_01589

Sites

Binding site381S-adenosyl-L-methionine
Binding site881S-adenosyl-L-methionine
Binding site1311S-adenosyl-L-methionine
Binding site1981Substrate Potential

Secondary structure

..................................... 241
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43985 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 08743633A3E853F8

FASTA24127,372
        10         20         30         40         50         60 
MVKDTLFSTP IAKLGDFIFD ENVAEVFPDM IQRSVPGYSN IITAIGMLAE RFVTADSNVY 

        70         80         90        100        110        120 
DLGCSRGAAT LSARRNINQP NVKIIGIDNS QPMVERCRQH IAAYHSEIPV EILCNDIRHV 

       130        140        150        160        170        180 
EIKNASMVIL NFTLQFLPPE DRIALLTKIY EGLNPNGVLV LSEKFRFEDT KINHLLIDLH 

       190        200        210        220        230        240 
HQFKRANGYS ELEVSQKRTA LENVMRTDSI ETHKVRLKNV GFSQVELWFQ CFNFGSMIAV 


K 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd."
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. expand/collapse author list , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C.
Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine."
Lim K., Zhang H., Tempczyk A., Bonander N., Toedt J., Howard A., Eisenstein E., Herzberg O.
Proteins 45:397-407(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE, FUNCTION, SUBUNIT.
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC21983.1.
PIRC64006.
RefSeqNP_438485.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IM8X-ray2.20A/B1-241[»]
ProteinModelPortalP43985.
SMRP43985. Positions 17-241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC21983; AAC21983; HI_0319.
GeneID949432.
KEGGhin:HI0319.
PATRIC20189181. VBIHaeInf48452_0337.

Phylogenomic databases

eggNOGCOG0500.
KOK15256.
OMADMLERSI.
OrthoDBEOG628F44.
ProtClustDBCLSK870321.

Family and domain databases

HAMAPMF_01589. tRNA_methyltr_CmoA.
InterProIPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PANTHERPTHR10108:SF119. PTHR10108:SF119. 1 hit.
PIRSFPIRSF006325. MeTrfase_bac. 1 hit.
TIGRFAMsTIGR00740. TIGR00740. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP43985.

Entry information

Entry nameCMOA_HAEIN
AccessionPrimary (citable) accession number: P43985
Secondary accession number(s): P43986
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names