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Protein

tRNA (cmo5U34)-methyltransferase

Gene

cmoA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381S-adenosyl-L-methionine
Binding sitei88 – 881S-adenosyl-L-methionine
Binding sitei131 – 1311S-adenosyl-L-methionine
Binding sitei198 – 1981SubstrateSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cmo5U34)-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:cmoA
Ordered Locus Names:HI_0319
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241tRNA (cmo5U34)-methyltransferasePRO_0000169084Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0319.

Structurei

Secondary structure

1
241
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 3414Combined sources
Helixi38 – 5215Combined sources
Beta strandi58 – 636Combined sources
Helixi68 – 758Combined sources
Beta strandi83 – 875Combined sources
Helixi91 – 10212Combined sources
Beta strandi110 – 1134Combined sources
Turni117 – 1193Combined sources
Beta strandi124 – 1329Combined sources
Helixi134 – 1363Combined sources
Helixi139 – 1413Combined sources
Helixi142 – 15211Combined sources
Beta strandi153 – 16412Combined sources
Helixi170 – 18617Combined sources
Helixi189 – 1913Combined sources
Helixi195 – 20410Combined sources
Helixi210 – 22011Combined sources
Beta strandi223 – 2319Combined sources
Beta strandi234 – 2407Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IM8X-ray2.20A/B1-241[»]
ProteinModelPortaliP43985.
SMRiP43985. Positions 17-241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43985.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 653S-adenosyl-L-methionine binding
Regioni116 – 1172S-adenosyl-L-methionine binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
KOiK15256.
OMAiPWFQCLN.
OrthoDBiEOG628F44.
PhylomeDBiP43985.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. tRNA_methyltr_CmoA.
InterProiIPR029063. SAM-dependent_MTases.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

Sequencei

Sequence statusi: Complete.

P43985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKDTLFSTP IAKLGDFIFD ENVAEVFPDM IQRSVPGYSN IITAIGMLAE
60 70 80 90 100
RFVTADSNVY DLGCSRGAAT LSARRNINQP NVKIIGIDNS QPMVERCRQH
110 120 130 140 150
IAAYHSEIPV EILCNDIRHV EIKNASMVIL NFTLQFLPPE DRIALLTKIY
160 170 180 190 200
EGLNPNGVLV LSEKFRFEDT KINHLLIDLH HQFKRANGYS ELEVSQKRTA
210 220 230 240
LENVMRTDSI ETHKVRLKNV GFSQVELWFQ CFNFGSMIAV K
Length:241
Mass (Da):27,372
Last modified:November 1, 1995 - v1
Checksum:i08743633A3E853F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21983.1.
PIRiC64006.
RefSeqiNP_438485.1. NC_000907.1.
WP_005694346.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21983; AAC21983; HI_0319.
GeneIDi949432.
KEGGihin:HI0319.
PATRICi20189181. VBIHaeInf48452_0337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21983.1.
PIRiC64006.
RefSeqiNP_438485.1. NC_000907.1.
WP_005694346.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IM8X-ray2.20A/B1-241[»]
ProteinModelPortaliP43985.
SMRiP43985. Positions 17-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21983; AAC21983; HI_0319.
GeneIDi949432.
KEGGihin:HI0319.
PATRICi20189181. VBIHaeInf48452_0337.

Phylogenomic databases

eggNOGiCOG0500.
KOiK15256.
OMAiPWFQCLN.
OrthoDBiEOG628F44.
PhylomeDBiP43985.

Miscellaneous databases

EvolutionaryTraceiP43985.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. tRNA_methyltr_CmoA.
InterProiIPR029063. SAM-dependent_MTases.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine."
    Lim K., Zhang H., Tempczyk A., Bonander N., Toedt J., Howard A., Eisenstein E., Herzberg O.
    Proteins 45:397-407(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE, FUNCTION, SUBUNIT.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiCMOA_HAEIN
AccessioniPrimary (citable) accession number: P43985
Secondary accession number(s): P43986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.