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P43985

- CMOA_HAEIN

UniProt

P43985 - CMOA_HAEIN

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Protein
tRNA (cmo5U34)-methyltransferase
Gene
cmoA, HI_0319
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) By similarity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381S-adenosyl-L-methionine
Binding sitei88 – 881S-adenosyl-L-methionine
Binding sitei131 – 1311S-adenosyl-L-methionine
Binding sitei198 – 1981Substrate Reviewed prediction

GO - Molecular functioni

  1. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA (uracil) methyltransferase activity Source: InterPro

GO - Biological processi

  1. tRNA wobble uridine modification Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cmo5U34)-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:cmoA
Ordered Locus Names:HI_0319
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241tRNA (cmo5U34)-methyltransferaseUniRule annotation
PRO_0000169084Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0319.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 3414
Helixi38 – 5215
Beta strandi58 – 636
Helixi68 – 758
Beta strandi83 – 875
Helixi91 – 10212
Beta strandi110 – 1134
Turni117 – 1193
Beta strandi124 – 1329
Helixi134 – 1363
Helixi139 – 1413
Helixi142 – 15211
Beta strandi153 – 16412
Helixi170 – 18617
Helixi189 – 1913
Helixi195 – 20410
Helixi210 – 22011
Beta strandi223 – 2319
Beta strandi234 – 2407

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IM8X-ray2.20A/B1-241[»]
ProteinModelPortaliP43985.
SMRiP43985. Positions 17-241.

Miscellaneous databases

EvolutionaryTraceiP43985.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 653S-adenosyl-L-methionine bindingUniRule annotation
Regioni116 – 1172S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
KOiK15256.
OMAiYDLGCSR.
OrthoDBiEOG628F44.
PhylomeDBiP43985.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01589. tRNA_methyltr_CmoA.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view]
PIRSFiPIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00740. TIGR00740. 1 hit.

Sequencei

Sequence statusi: Complete.

P43985-1 [UniParc]FASTAAdd to Basket

« Hide

MVKDTLFSTP IAKLGDFIFD ENVAEVFPDM IQRSVPGYSN IITAIGMLAE    50
RFVTADSNVY DLGCSRGAAT LSARRNINQP NVKIIGIDNS QPMVERCRQH 100
IAAYHSEIPV EILCNDIRHV EIKNASMVIL NFTLQFLPPE DRIALLTKIY 150
EGLNPNGVLV LSEKFRFEDT KINHLLIDLH HQFKRANGYS ELEVSQKRTA 200
LENVMRTDSI ETHKVRLKNV GFSQVELWFQ CFNFGSMIAV K 241
Length:241
Mass (Da):27,372
Last modified:November 1, 1995 - v1
Checksum:i08743633A3E853F8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21983.1.
PIRiC64006.
RefSeqiNP_438485.1. NC_000907.1.
WP_005694346.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21983; AAC21983; HI_0319.
GeneIDi949432.
KEGGihin:HI0319.
PATRICi20189181. VBIHaeInf48452_0337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21983.1 .
PIRi C64006.
RefSeqi NP_438485.1. NC_000907.1.
WP_005694346.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IM8 X-ray 2.20 A/B 1-241 [» ]
ProteinModelPortali P43985.
SMRi P43985. Positions 17-241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0319.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC21983 ; AAC21983 ; HI_0319 .
GeneIDi 949432.
KEGGi hin:HI0319.
PATRICi 20189181. VBIHaeInf48452_0337.

Phylogenomic databases

eggNOGi COG0500.
KOi K15256.
OMAi YDLGCSR.
OrthoDBi EOG628F44.
PhylomeDBi P43985.

Miscellaneous databases

EvolutionaryTracei P43985.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01589. tRNA_methyltr_CmoA.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR005271. tRNA_cmo5U34_MeTrfase.
[Graphical view ]
PIRSFi PIRSF006325. MeTrfase_bac. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00740. TIGR00740. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Crystal structure of YecO from Haemophilus influenzae (HI0319) reveals a methyltransferase fold and a bound S-adenosylhomocysteine."
    Lim K., Zhang H., Tempczyk A., Bonander N., Toedt J., Howard A., Eisenstein E., Herzberg O.
    Proteins 45:397-407(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE, FUNCTION, SUBUNIT.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiCMOA_HAEIN
AccessioniPrimary (citable) accession number: P43985
Secondary accession number(s): P43986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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