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Protein

UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase

Gene

mpl

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramate.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi111 – 1177ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00544.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligaseUniRule annotation (EC:6.3.2.45UniRule annotation)
Alternative name(s):
Murein peptide ligaseUniRule annotation
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligaseUniRule annotation
Gene namesi
Name:mplUniRule annotation
Ordered Locus Names:HI_0121
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligasePRO_0000101709Add
BLAST

Proteomic databases

PRIDEiP43948.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0121.

Structurei

3D structure databases

ProteinModelPortaliP43948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family. Mpl subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0773.
KOiK02558.
OMAiPPMSDQL.
OrthoDBiEOG64BQ73.
PhylomeDBiP43948.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02020. Mpl.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005757. Murein_peptide_ligase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01081. mpl. 1 hit.

Sequencei

Sequence statusi: Complete.

P43948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHIHILGIC GTFMGGVAMI AKQMGYHVTG SDTNVYPPMS TFLEEQGIEI
60 70 80 90 100
IPNYDVAQLQ PAPDMVIVGN AMKRGNPCVE YVLENNLKYT SGPQWLHDHL
110 120 130 140 150
LRDRWVLAVS GTHGKTTTTG MLTWVLEQNG LKSGFLIGGI AGNFGISARL
160 170 180 190 200
GDSPYFIIEA DEYDTAFFDK RSKFVHYNPR TLIVNNISFD HADIFDDLKA
210 220 230 240 250
IQRQFHHMIR TIPASGLVLS SASEQSAKET LALGCWSQQQ FLGKDNEWFA
260 270 280 290 300
ERITNDASHF AVFHHGEKVA EVKWNVVGQH NMHNALMAIA AAHHTGVAIE
310 320 330 340 350
DACKALGSFV NAKRRLEVKG EVNSITVYDD FAHHPEAILA TLTALRDKVG
360 370 380 390 400
GGVRILAVLE PRSNTMKMGV HKDDIAPALG RADAVFMLQP EQLSWEVADI
410 420 430 440 450
ANQCVQPAYW NANLDRLVDM IVAKAQPTDH ILVMSNGSFG GIHQKILDKL

KLK
Length:453
Mass (Da):50,074
Last modified:November 1, 1995 - v1
Checksum:i5E7E71A07646EB7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21795.1.
PIRiB64002.
RefSeqiNP_438293.1. NC_000907.1.
WP_005694412.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21795; AAC21795; HI_0121.
GeneIDi951026.
KEGGihin:HI0121.
PATRICi20188727. VBIHaeInf48452_0125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21795.1.
PIRiB64002.
RefSeqiNP_438293.1. NC_000907.1.
WP_005694412.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0121.

Proteomic databases

PRIDEiP43948.

Protocols and materials databases

DNASUi951026.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21795; AAC21795; HI_0121.
GeneIDi951026.
KEGGihin:HI0121.
PATRICi20188727. VBIHaeInf48452_0125.

Phylogenomic databases

eggNOGiCOG0773.
KOiK02558.
OMAiPPMSDQL.
OrthoDBiEOG64BQ73.
PhylomeDBiP43948.

Enzyme and pathway databases

UniPathwayiUPA00544.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02020. Mpl.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005757. Murein_peptide_ligase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01081. mpl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiMPL_HAEIN
AccessioniPrimary (citable) accession number: P43948
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.