P43948 (MPL_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
EC=6.3.2.-

Alternative name(s):
Murein peptide ligase

Gene names

Name:	mpl
Ordered Locus Names:	HI_0121

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid.
Pathway	Cell wall biogenesis; peptidoglycan recycling.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Secreted
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acid-amino acid ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase		PRO_0000101709
Regions
	Nucleotide binding	111 – 117	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P43948 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5E7E71A07646EB7D	FASTA	453	50,074
	10 20 30 40 50 60 MKHIHILGIC GTFMGGVAMI AKQMGYHVTG SDTNVYPPMS TFLEEQGIEI IPNYDVAQLQ 70 80 90 100 110 120 PAPDMVIVGN AMKRGNPCVE YVLENNLKYT SGPQWLHDHL LRDRWVLAVS GTHGKTTTTG 130 140 150 160 170 180 MLTWVLEQNG LKSGFLIGGI AGNFGISARL GDSPYFIIEA DEYDTAFFDK RSKFVHYNPR 190 200 210 220 230 240 TLIVNNISFD HADIFDDLKA IQRQFHHMIR TIPASGLVLS SASEQSAKET LALGCWSQQQ 250 260 270 280 290 300 FLGKDNEWFA ERITNDASHF AVFHHGEKVA EVKWNVVGQH NMHNALMAIA AAHHTGVAIE 310 320 330 340 350 360 DACKALGSFV NAKRRLEVKG EVNSITVYDD FAHHPEAILA TLTALRDKVG GGVRILAVLE 370 380 390 400 410 420 PRSNTMKMGV HKDDIAPALG RADAVFMLQP EQLSWEVADI ANQCVQPAYW NANLDRLVDM 430 440 450 IVAKAQPTDH ILVMSNGSFG GIHQKILDKL KLK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21795.1.
PIR	B64002.
RefSeq	NP_438293.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43948.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0121.
Proteomic databases
PRIDE	P43948.
Protocols and materials databases
DNASU	951026.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC21795; AAC21795; HI_0121.
GeneID	951026.
KEGG	hin:HI0121.
PATRIC	20188727. VBIHaeInf48452_0125.
Phylogenomic databases
eggNOG	COG0773.
KO	K02558.
OMA	PYTSGPQ.
ProtClustDB	CLSK870302.
Enzyme and pathway databases
UniPathway	UPA00544.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.40.50.720. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005757. Murein_peptide_ligase. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23135:SF8. PTHR23135:SF8. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01081. mpl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MPL_HAEIN

Accession

Primary (citable) accession number: P43948

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program