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Protein

Pyruvate kinase

Gene

pykA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK (By similarity).By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi38PotassiumBy similarity1
Metal bindingi40PotassiumBy similarity1
Metal bindingi70PotassiumBy similarity1
Sitei223Transition state stabilizerBy similarity1
Metal bindingi225MagnesiumBy similarity1
Binding sitei251Substrate; via amide nitrogenBy similarity1
Metal bindingi252MagnesiumBy similarity1
Binding sitei252Substrate; via amide nitrogenBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-1591-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pykA
Ordered Locus Names:HI_1573
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001120752 – 478Pyruvate kinaseAdd BLAST477

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1573

Structurei

3D structure databases

ProteinModelPortaliP43924
SMRiP43924
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
KOiK00873
OMAiICVEAEK
PhylomeDBiP43924

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRLRRTKI VCTMGPSTDR DNNLEKIIAA GANVVRMNFS HGTPDDHIGR
60 70 80 90 100
AERVRSIAKK LGKTVAILGD LQGPKIRVST FKDGKIFLNV GDKFILDAEL
110 120 130 140 150
PKGEGTQESV GLDYKTLPQD VVPGDILLLD DGRVQLKVLS TDGAKVFTEV
160 170 180 190 200
TVGGPLSNNK GINKLGGGLS ADALTEKDKA DIITAARIGV DFLAVSFPRS
210 220 230 240 250
SADLNYAREL AQQAGLNAKI VAKVERAETV ANDEAMDDII LASDVIMVAR
260 270 280 290 300
GDLGVEIGDP ELVGVQKKLI RRSRQLNRAV ITATQMMESM ISNPMPTRAE
310 320 330 340 350
VMDVANAVLD GTDAVMLSAE TAAGQYPSET VAAMASVCLG AEKMPSINVS
360 370 380 390 400
RHRMDKEFET IEESVAMSAM YAANHMKGVA AIVTLSSTGR TPLLMSRISS
410 420 430 440 450
GLPIFALSRN QETLNLCALY RGVTPIYHGE ESRTEAGAKA APQSLKEKGY
460 470
LSTGDLVLVT QGGQGATQTN VCRTLIVE
Length:478
Mass (Da):51,055
Last modified:January 23, 2007 - v2
Checksum:iFF803EC3E3BD6216
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC23216.1
PIRiC64130
RefSeqiNP_439719.1, NC_000907.1
WP_010869247.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC23216; AAC23216; HI_1573
GeneIDi950433
KEGGihin:HI1573
PATRICifig|71421.8.peg.1645

Similar proteinsi

Entry informationi

Entry nameiKPYK_HAEIN
AccessioniPrimary (citable) accession number: P43924
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health