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P43923 (PCKA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]

Short name=PCK
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckA
Ordered Locus Names:HI_0809
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA By similarity. HAMAP-Rule MF_00453

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00453

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00453.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandATP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (ATP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 538538Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453
PRO_0000203824

Regions

Nucleotide binding246 – 2549ATP By similarity
Nucleotide binding447 – 4482ATP By similarity

Sites

Metal binding2111Manganese By similarity
Metal binding2301Manganese; via tele nitrogen By similarity
Metal binding2671Manganese By similarity
Binding site641Substrate By similarity
Binding site2051Substrate By similarity
Binding site2111ATP By similarity
Binding site2111Substrate By similarity
Binding site2301ATP By similarity
Binding site2951ATP By similarity
Binding site3311ATP By similarity
Binding site3311Substrate By similarity
Binding site4531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P43923 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: EF7AD503933DB3E6

FASTA53859,404
        10         20         30         40         50         60 
MTDLNKVVKE LEALGIYDVK EVVYNPSYEQ LFEEETKPGL EGFEKGTLTT TGAVAVDTGI 

        70         80         90        100        110        120 
FTGRSPKDKY IVLDEKTKDT VWWTSETAKN DNKPMNQATW QSLKDLVTNQ LSRKRLFVVD 

       130        140        150        160        170        180 
GFCGASEHDR IAVRIVTEVA WQAHFVKNMF IRPTEEQLKN FEPDFVVMNG SKVTNPNWKE 

       190        200        210        220        230        240 
QGLNSENFVA FNLTERIQLI GGTWYGGEMK KGMSSMMNYF LPLKGVGAMH CSANVGKDGD 

       250        260        270        280        290        300 
VAIFFGLSGT GKTTLSTDPK RELIGDDEHG WDDVGIFNFE GGCYAKTIHL SEENEPDIYR 

       310        320        330        340        350        360 
AIRRDALLEN VVVRSDGSVD FDDGSKTENT RVSYPIYHID NIVKPVSRAG HATKVIFLTA 

       370        380        390        400        410        420 
DAFGVLPPVS KLTPEQTKYY FLSGFTAKLA GTERGITEPT PTFSACFGAA FLTLHPTQYA 

       430        440        450        460        470        480 
EVLVKRMQAA GAEAYLVNTG WNGTGKRISI KDTRGIIDAI LDGSIEKAEM GELPIFNLAI 

       490        500        510        520        530 
PKALPGVDSA ILDPRDTYAD KAQWQSKAED LAGRFVKNFV KYATNEEGKA LIAAGPKA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22468.1.
PIRE64095.
RefSeqNP_438969.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43923.
SMRP43923. Positions 3-537.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0809.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22468; AAC22468; HI_0809.
GeneID950742.
KEGGhin:HI0809.
PATRIC20190273. VBIHaeInf48452_0850.

Phylogenomic databases

eggNOGCOG1866.
KOK01610.
OMARYAGEMK.
OrthoDBEOG6DG2RK.
ProtClustDBPRK09344.

Enzyme and pathway databases

UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00453. PEPCK_ATP.
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKA_HAEIN
AccessionPrimary (citable) accession number: P43923
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names