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P43919 (GLND_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme

Short name=UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein

Including the following 2 domains:

  1. [Protein-PII] uridylyltransferase
    Short name=PII uridylyltransferase
    Short name=UTase
    EC=2.7.7.59
  2. [Protein-PII]-UMP uridylyl-removing enzyme
    Short name=UR
    EC=3.1.4.-
Gene names
Name:glnD
Ordered Locus Names:HI_1719
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family.

Contains 2 ACT domains.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277
PRO_0000192736

Regions

Domain447 – 579133HD
Domain688 – 76477ACT 1
Domain794 – 86370ACT 2
Region1 – 328328Uridylyltransferase HAMAP-Rule MF_00277
Region329 – 687359Uridylyl-removing HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
P43919 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: B6F679A70A01A651

FASTA863100,173
        10         20         30         40         50         60 
MLFSPTLSSL LTPSAVKIER ENLKQFELEN FSCYSIFELI ENRCDFYDAL LIQLWQEIGL 

        70         80         90        100        110        120 
SEQQGISLIA VGGYGRREMF PLSDLDFLIL VEQTPSHEIE EKITQFIQFL WDCGFEVGNS 

       130        140        150        160        170        180 
VRTLEQCELE GKQDITIATN LLEARFLTGN RPHFDVLNEL VKRADFWSKE DFFNAKVQEQ 

       190        200        210        220        230        240 
IERYQRYHNT AYNLEPDIKF SPGGLRDLHL LYWVALRHSG ALTLEAILQS GFIYPQEYQQ 

       250        260        270        280        290        300 
LQESRAFLFK VRFALHLILK RYDNRLLFDR QIKVSELLGF RGEGNPAVEK MMKCFFQALH 

       310        320        330        340        350        360 
RISLISNLLI QHYRENVLSS NQDTVIDQLD DDFQLINQSL CLRNSFVFQE KPARILDLFF 

       370        380        390        400        410        420 
YLTQYEHVNI HSDTLRQLQI SLEQLSQKLC EIPAAREKFL RLFNQSNAIK RAFMPMHQYG 

       430        440        450        460        470        480 
VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT LRVMLKLESF LPKGSAQEHP IAHRIFSQLS 

       490        500        510        520        530        540 
DRTLLYIAAL FHDIAKGRGG DHAELGAEDV ADFAQLHGLD RREIDTLAWL VQSHLLMSIT 

       550        560        570        580        590        600 
AQRRDIHDPE VVMNFAEAMQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTE 

       610        620        630        640        650        660 
QQFSQGMKEL LDYSEKSAEN RKLAQQILTQ DYSDITSISI EKLWTRCPED YFVRNTPKQI 

       670        680        690        700        710        720 
AWHTSLLVDF VEALLVKISN RFSLGGTEVF IYCQDQPHLF NKVVSTIGAK KFSIHDAQII 

       730        740        750        760        770        780 
TTQDGYVFDS FIITELNGEL VEFDRRRELE QALTVALQSE KLPALSIVPN RQLQHFTVQT 

       790        800        810        820        830        840 
DVRFLQENKK EHTEMELVAL DKAGLLAQVS QIFTELNLNL LNAKITTVGE KAEDFFILTN 

       850        860 
QFGQALAREE RERLNSVIIQ QIR 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC23362.1.
PIRB64138.
RefSeqNP_439860.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43919.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1719.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC23362; AAC23362; HI_1719.
GeneID950533.
KEGGhin:HI1719.
PATRIC20192191. VBIHaeInf48452_1798.

Phylogenomic databases

eggNOGCOG2844.
KOK00990.
OMASPKVWNA.
OrthoDBEOG6CCH44.
ProtClustDBPRK01759.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PANTHERPTHR13734:SF1. PTHR13734:SF1. 1 hit.
PfamPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLND_HAEIN
AccessionPrimary (citable) accession number: P43919
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names