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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:HI_1719
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192736Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1719.

Structurei

3D structure databases

ProteinModelPortaliP43919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini447 – 579133HDUniRule annotationAdd
BLAST
Domaini688 – 76477ACT 1UniRule annotationAdd
BLAST
Domaini794 – 86370ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 328328UridylyltransferaseAdd
BLAST
Regioni329 – 687359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.
PhylomeDBiP43919.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43919-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFSPTLSSL LTPSAVKIER ENLKQFELEN FSCYSIFELI ENRCDFYDAL
60 70 80 90 100
LIQLWQEIGL SEQQGISLIA VGGYGRREMF PLSDLDFLIL VEQTPSHEIE
110 120 130 140 150
EKITQFIQFL WDCGFEVGNS VRTLEQCELE GKQDITIATN LLEARFLTGN
160 170 180 190 200
RPHFDVLNEL VKRADFWSKE DFFNAKVQEQ IERYQRYHNT AYNLEPDIKF
210 220 230 240 250
SPGGLRDLHL LYWVALRHSG ALTLEAILQS GFIYPQEYQQ LQESRAFLFK
260 270 280 290 300
VRFALHLILK RYDNRLLFDR QIKVSELLGF RGEGNPAVEK MMKCFFQALH
310 320 330 340 350
RISLISNLLI QHYRENVLSS NQDTVIDQLD DDFQLINQSL CLRNSFVFQE
360 370 380 390 400
KPARILDLFF YLTQYEHVNI HSDTLRQLQI SLEQLSQKLC EIPAAREKFL
410 420 430 440 450
RLFNQSNAIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT
460 470 480 490 500
LRVMLKLESF LPKGSAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG
510 520 530 540 550
DHAELGAEDV ADFAQLHGLD RREIDTLAWL VQSHLLMSIT AQRRDIHDPE
560 570 580 590 600
VVMNFAEAMQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTE
610 620 630 640 650
QQFSQGMKEL LDYSEKSAEN RKLAQQILTQ DYSDITSISI EKLWTRCPED
660 670 680 690 700
YFVRNTPKQI AWHTSLLVDF VEALLVKISN RFSLGGTEVF IYCQDQPHLF
710 720 730 740 750
NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE
760 770 780 790 800
QALTVALQSE KLPALSIVPN RQLQHFTVQT DVRFLQENKK EHTEMELVAL
810 820 830 840 850
DKAGLLAQVS QIFTELNLNL LNAKITTVGE KAEDFFILTN QFGQALAREE
860
RERLNSVIIQ QIR
Length:863
Mass (Da):100,173
Last modified:November 1, 1995 - v1
Checksum:iB6F679A70A01A651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23362.1.
PIRiB64138.
RefSeqiNP_439860.1. NC_000907.1.
WP_005694204.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23362; AAC23362; HI_1719.
GeneIDi950533.
KEGGihin:HI1719.
PATRICi20192191. VBIHaeInf48452_1798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23362.1.
PIRiB64138.
RefSeqiNP_439860.1. NC_000907.1.
WP_005694204.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23362; AAC23362; HI_1719.
GeneIDi950533.
KEGGihin:HI1719.
PATRICi20192191. VBIHaeInf48452_1798.

Phylogenomic databases

eggNOGiCOG2844.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.
PhylomeDBiP43919.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiGLND_HAEIN
AccessioniPrimary (citable) accession number: P43919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.