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P43919

- GLND_HAEIN

UniProt

P43919 - GLND_HAEIN

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:HI_1719
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192736Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1719.

    Structurei

    3D structure databases

    ProteinModelPortaliP43919.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini447 – 579133HDUniRule annotationAdd
    BLAST
    Domaini688 – 76477ACT 1UniRule annotationAdd
    BLAST
    Domaini794 – 86370ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 328328UridylyltransferaseAdd
    BLAST
    Regioni329 – 687359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.
    PhylomeDBiP43919.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43919-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLFSPTLSSL LTPSAVKIER ENLKQFELEN FSCYSIFELI ENRCDFYDAL    50
    LIQLWQEIGL SEQQGISLIA VGGYGRREMF PLSDLDFLIL VEQTPSHEIE 100
    EKITQFIQFL WDCGFEVGNS VRTLEQCELE GKQDITIATN LLEARFLTGN 150
    RPHFDVLNEL VKRADFWSKE DFFNAKVQEQ IERYQRYHNT AYNLEPDIKF 200
    SPGGLRDLHL LYWVALRHSG ALTLEAILQS GFIYPQEYQQ LQESRAFLFK 250
    VRFALHLILK RYDNRLLFDR QIKVSELLGF RGEGNPAVEK MMKCFFQALH 300
    RISLISNLLI QHYRENVLSS NQDTVIDQLD DDFQLINQSL CLRNSFVFQE 350
    KPARILDLFF YLTQYEHVNI HSDTLRQLQI SLEQLSQKLC EIPAAREKFL 400
    RLFNQSNAIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT 450
    LRVMLKLESF LPKGSAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG 500
    DHAELGAEDV ADFAQLHGLD RREIDTLAWL VQSHLLMSIT AQRRDIHDPE 550
    VVMNFAEAMQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTE 600
    QQFSQGMKEL LDYSEKSAEN RKLAQQILTQ DYSDITSISI EKLWTRCPED 650
    YFVRNTPKQI AWHTSLLVDF VEALLVKISN RFSLGGTEVF IYCQDQPHLF 700
    NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE 750
    QALTVALQSE KLPALSIVPN RQLQHFTVQT DVRFLQENKK EHTEMELVAL 800
    DKAGLLAQVS QIFTELNLNL LNAKITTVGE KAEDFFILTN QFGQALAREE 850
    RERLNSVIIQ QIR 863
    Length:863
    Mass (Da):100,173
    Last modified:November 1, 1995 - v1
    Checksum:iB6F679A70A01A651
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23362.1.
    PIRiB64138.
    RefSeqiNP_439860.1. NC_000907.1.
    WP_005694204.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC23362; AAC23362; HI_1719.
    GeneIDi950533.
    KEGGihin:HI1719.
    PATRICi20192191. VBIHaeInf48452_1798.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC23362.1 .
    PIRi B64138.
    RefSeqi NP_439860.1. NC_000907.1.
    WP_005694204.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P43919.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC23362 ; AAC23362 ; HI_1719 .
    GeneIDi 950533.
    KEGGi hin:HI1719.
    PATRICi 20192191. VBIHaeInf48452_1798.

    Phylogenomic databases

    eggNOGi COG2844.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.
    PhylomeDBi P43919.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiGLND_HAEIN
    AccessioniPrimary (citable) accession number: P43919
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3