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P43912

- TRMD_HAEIN

UniProt

P43912 - TRMD_HAEIN

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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionine
Binding sitei113 – 1131S-adenosyl-L-methionine; via amide nitrogen
Active sitei169 – 1691Proton acceptorSequence Analysis

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene namesi
Name:trmD
Ordered Locus Names:HI_0202
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246tRNA (guanine-N(1)-)-methyltransferasePRO_0000060385Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0202.

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65
Helixi10 – 134
Helixi14 – 174
Helixi20 – 278
Beta strandi30 – 367
Helixi38 – 414
Helixi64 – 7815
Beta strandi83 – 875
Beta strandi91 – 933
Helixi96 – 1027
Beta strandi106 – 1116
Helixi120 – 1267
Beta strandi128 – 1336
Beta strandi139 – 1413
Helixi142 – 15312
Turni171 – 1744
Beta strandi185 – 1873
Helixi194 – 1985
Helixi201 – 21919
Helixi221 – 2255
Helixi231 – 24414

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAJX-ray1.85A1-246[»]
1UAKX-ray2.05A1-246[»]
1UALX-ray1.80A1-246[»]
1UAMX-ray2.20A1-246[»]
3AXZX-ray2.25A1-246[»]
4MCBX-ray1.94A/B1-246[»]
4MCCX-ray1.95A/B1-246[»]
4MCDX-ray1.55A1-246[»]
ProteinModelPortaliP43912.
SMRiP43912. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43912.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1386S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.Curated

Phylogenomic databases

eggNOGiCOG0336.
KOiK00554.
OMAiFVAGRYE.
OrthoDBiEOG6J48RZ.
PhylomeDBiP43912.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

P43912-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD
60 70 80 90 100
DRPYGGGPGM LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT
110 120 130 140 150
ELAQNQKLIL VCGRYEGIDE RLIQTEIDEE WSIGDYVLTG GELPAMTLID
160 170 180 190 200
AVARFIPGVL GKQASAEEDS FADGLLDCPH YTRPEVLEGL TVPPVLMSGH
210 220 230 240
HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA QAEHNS
Length:246
Mass (Da):27,543
Last modified:November 1, 1995 - v1
Checksum:iDEEF238159B1003D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21871.1.
PIRiC64054.
RefSeqiNP_438371.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21871; AAC21871; HI_0202.
GeneIDi951111.
KEGGihin:HI0202.
PATRICi20188901. VBIHaeInf48452_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC21871.1 .
PIRi C64054.
RefSeqi NP_438371.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UAJ X-ray 1.85 A 1-246 [» ]
1UAK X-ray 2.05 A 1-246 [» ]
1UAL X-ray 1.80 A 1-246 [» ]
1UAM X-ray 2.20 A 1-246 [» ]
3AXZ X-ray 2.25 A 1-246 [» ]
4MCB X-ray 1.94 A/B 1-246 [» ]
4MCC X-ray 1.95 A/B 1-246 [» ]
4MCD X-ray 1.55 A 1-246 [» ]
ProteinModelPortali P43912.
SMRi P43912. Positions 1-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0202.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC21871 ; AAC21871 ; HI_0202 .
GeneIDi 951111.
KEGGi hin:HI0202.
PATRICi 20188901. VBIHaeInf48452_0207.

Phylogenomic databases

eggNOGi COG0336.
KOi K00554.
OMAi FVAGRYE.
OrthoDBi EOG6J48RZ.
PhylomeDBi P43912.

Miscellaneous databases

EvolutionaryTracei P43912.

Family and domain databases

Gene3Di 1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPi MF_00605. TrmD.
InterProi IPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view ]
Pfami PF01746. tRNA_m1G_MT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000386. tRNA_mtase. 1 hit.
SUPFAMi SSF75217. SSF75217. 1 hit.
TIGRFAMsi TIGR00088. trmD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition."
    Ahn H.J., Kim H.-W., Yoon H.-J., Lee B.I., Suh S.W., Yang J.K.
    EMBO J. 22:2593-2603(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, SUBUNIT.

Entry informationi

Entry nameiTRMD_HAEIN
AccessioniPrimary (citable) accession number: P43912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3