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P43912 (TRMD_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:HI_0202
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer. Ref.2

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_0000060385

Regions

Region133 – 1386S-adenosyl-L-methionine binding HAMAP-Rule MF_00605

Sites

Active site1691Proton acceptor Potential
Binding site861S-adenosyl-L-methionine
Binding site1131S-adenosyl-L-methionine; via amide nitrogen

Secondary structure

......................................... 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43912 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: DEEF238159B1003D

FASTA24627,543
        10         20         30         40         50         60 
MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD DRPYGGGPGM 

        70         80         90        100        110        120 
LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT ELAQNQKLIL VCGRYEGIDE 

       130        140        150        160        170        180 
RLIQTEIDEE WSIGDYVLTG GELPAMTLID AVARFIPGVL GKQASAEEDS FADGLLDCPH 

       190        200        210        220        230        240 
YTRPEVLEGL TVPPVLMSGH HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA 


QAEHNS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC21871.1.
PIRC64054.
RefSeqNP_438371.1. NC_000907.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAJX-ray1.85A1-246[»]
1UAKX-ray2.05A1-246[»]
1UALX-ray1.80A1-246[»]
1UAMX-ray2.20A1-246[»]
3AXZX-ray2.25A1-246[»]
4MCBX-ray1.94A/B1-246[»]
4MCCX-ray1.95A/B1-246[»]
4MCDX-ray1.55A1-246[»]
ProteinModelPortalP43912.
SMRP43912. Positions 1-246.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC21871; AAC21871; HI_0202.
GeneID951111.
KEGGhin:HI0202.
PATRIC20188901. VBIHaeInf48452_0207.

Phylogenomic databases

eggNOGCOG0336.
KOK00554.
OMAFVAGRYE.
OrthoDBEOG6J48RZ.
PhylomeDBP43912.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMSSF75217. SSF75217. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP43912.

Entry information

Entry nameTRMD_HAEIN
AccessionPrimary (citable) accession number: P43912
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names