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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionine
Binding sitei113 – 1131S-adenosyl-L-methionine; via amide nitrogen
Active sitei169 – 1691Proton acceptorSequence Analysis

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.228. 2529.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene namesi
Name:trmD
Ordered Locus Names:HI_0202
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246tRNA (guanine-N(1)-)-methyltransferasePRO_0000060385Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0202.

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 134Combined sources
Helixi14 – 174Combined sources
Helixi20 – 278Combined sources
Beta strandi30 – 367Combined sources
Helixi38 – 414Combined sources
Helixi64 – 7815Combined sources
Beta strandi83 – 875Combined sources
Beta strandi91 – 933Combined sources
Helixi96 – 1027Combined sources
Beta strandi106 – 1116Combined sources
Helixi120 – 1267Combined sources
Beta strandi128 – 1336Combined sources
Beta strandi139 – 1413Combined sources
Helixi142 – 15312Combined sources
Turni171 – 1744Combined sources
Beta strandi185 – 1873Combined sources
Helixi194 – 1985Combined sources
Helixi201 – 21919Combined sources
Helixi221 – 2255Combined sources
Helixi231 – 24414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAJX-ray1.85A1-246[»]
1UAKX-ray2.05A1-246[»]
1UALX-ray1.80A1-246[»]
1UAMX-ray2.20A1-246[»]
3AXZX-ray2.25A1-246[»]
4MCBX-ray1.94A/B1-246[»]
4MCCX-ray1.95A/B1-246[»]
4MCDX-ray1.55A1-246[»]
ProteinModelPortaliP43912.
SMRiP43912. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43912.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1386S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.Curated

Phylogenomic databases

eggNOGiCOG0336.
KOiK00554.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.
PhylomeDBiP43912.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

P43912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD
60 70 80 90 100
DRPYGGGPGM LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT
110 120 130 140 150
ELAQNQKLIL VCGRYEGIDE RLIQTEIDEE WSIGDYVLTG GELPAMTLID
160 170 180 190 200
AVARFIPGVL GKQASAEEDS FADGLLDCPH YTRPEVLEGL TVPPVLMSGH
210 220 230 240
HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA QAEHNS
Length:246
Mass (Da):27,543
Last modified:November 1, 1995 - v1
Checksum:iDEEF238159B1003D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21871.1.
PIRiC64054.
RefSeqiNP_438371.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21871; AAC21871; HI_0202.
GeneIDi951111.
KEGGihin:HI0202.
PATRICi20188901. VBIHaeInf48452_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21871.1.
PIRiC64054.
RefSeqiNP_438371.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAJX-ray1.85A1-246[»]
1UAKX-ray2.05A1-246[»]
1UALX-ray1.80A1-246[»]
1UAMX-ray2.20A1-246[»]
3AXZX-ray2.25A1-246[»]
4MCBX-ray1.94A/B1-246[»]
4MCCX-ray1.95A/B1-246[»]
4MCDX-ray1.55A1-246[»]
ProteinModelPortaliP43912.
SMRiP43912. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21871; AAC21871; HI_0202.
GeneIDi951111.
KEGGihin:HI0202.
PATRICi20188901. VBIHaeInf48452_0207.

Phylogenomic databases

eggNOGiCOG0336.
KOiK00554.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.
PhylomeDBiP43912.

Enzyme and pathway databases

BRENDAi2.1.1.228. 2529.

Miscellaneous databases

EvolutionaryTraceiP43912.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition."
    Ahn H.J., Kim H.-W., Yoon H.-J., Lee B.I., Suh S.W., Yang J.K.
    EMBO J. 22:2593-2603(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, SUBUNIT.

Entry informationi

Entry nameiTRMD_HAEIN
AccessioniPrimary (citable) accession number: P43912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.