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P43912

- TRMD_HAEIN

UniProt

P43912 - TRMD_HAEIN

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Protein
tRNA (guanine-N(1)-)-methyltransferase
Gene
trmD, HI_0202
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionine
Binding sitei113 – 1131S-adenosyl-L-methionine; via amide nitrogen
Active sitei169 – 1691Proton acceptor Reviewed prediction

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
    Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase
    Gene namesi
    Name:trmD
    Ordered Locus Names:HI_0202
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm Reviewed prediction UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation
    PRO_0000060385Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi71421.HI0202.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi10 – 134
    Helixi14 – 174
    Helixi20 – 278
    Beta strandi30 – 367
    Helixi38 – 414
    Helixi64 – 7815
    Beta strandi83 – 875
    Beta strandi91 – 933
    Helixi96 – 1027
    Beta strandi106 – 1116
    Helixi120 – 1267
    Beta strandi128 – 1336
    Beta strandi139 – 1413
    Helixi142 – 15312
    Turni171 – 1744
    Beta strandi185 – 1873
    Helixi194 – 1985
    Helixi201 – 21919
    Helixi221 – 2255
    Helixi231 – 24414

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UAJX-ray1.85A1-246[»]
    1UAKX-ray2.05A1-246[»]
    1UALX-ray1.80A1-246[»]
    1UAMX-ray2.20A1-246[»]
    3AXZX-ray2.25A1-246[»]
    4MCBX-ray1.94A/B1-246[»]
    4MCCX-ray1.95A/B1-246[»]
    4MCDX-ray1.55A1-246[»]
    ProteinModelPortaliP43912.
    SMRiP43912. Positions 1-246.

    Miscellaneous databases

    EvolutionaryTraceiP43912.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni133 – 1386S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0336.
    KOiK00554.
    OMAiFVAGRYE.
    OrthoDBiEOG6J48RZ.
    PhylomeDBiP43912.

    Family and domain databases

    Gene3Di1.10.1270.20. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPiMF_00605. TrmD.
    InterProiIPR029028. Alpha/beta_knot_MTases.
    IPR002649. tRNA_m1G_MeTrfase_bac.
    IPR023148. tRNA_m1G_MeTrfase_C.
    IPR029026. tRNA_m1G_MTases_N.
    IPR016009. tRNA_MeTrfase_TRMD/TRM10.
    [Graphical view]
    PfamiPF01746. tRNA_m1G_MT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
    SUPFAMiSSF75217. SSF75217. 1 hit.
    TIGRFAMsiTIGR00088. trmD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P43912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD    50
    DRPYGGGPGM LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT 100
    ELAQNQKLIL VCGRYEGIDE RLIQTEIDEE WSIGDYVLTG GELPAMTLID 150
    AVARFIPGVL GKQASAEEDS FADGLLDCPH YTRPEVLEGL TVPPVLMSGH 200
    HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA QAEHNS 246
    Length:246
    Mass (Da):27,543
    Last modified:November 1, 1995 - v1
    Checksum:iDEEF238159B1003D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21871.1.
    PIRiC64054.
    RefSeqiNP_438371.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC21871; AAC21871; HI_0202.
    GeneIDi951111.
    KEGGihin:HI0202.
    PATRICi20188901. VBIHaeInf48452_0207.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21871.1 .
    PIRi C64054.
    RefSeqi NP_438371.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UAJ X-ray 1.85 A 1-246 [» ]
    1UAK X-ray 2.05 A 1-246 [» ]
    1UAL X-ray 1.80 A 1-246 [» ]
    1UAM X-ray 2.20 A 1-246 [» ]
    3AXZ X-ray 2.25 A 1-246 [» ]
    4MCB X-ray 1.94 A/B 1-246 [» ]
    4MCC X-ray 1.95 A/B 1-246 [» ]
    4MCD X-ray 1.55 A 1-246 [» ]
    ProteinModelPortali P43912.
    SMRi P43912. Positions 1-246.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC21871 ; AAC21871 ; HI_0202 .
    GeneIDi 951111.
    KEGGi hin:HI0202.
    PATRICi 20188901. VBIHaeInf48452_0207.

    Phylogenomic databases

    eggNOGi COG0336.
    KOi K00554.
    OMAi FVAGRYE.
    OrthoDBi EOG6J48RZ.
    PhylomeDBi P43912.

    Miscellaneous databases

    EvolutionaryTracei P43912.

    Family and domain databases

    Gene3Di 1.10.1270.20. 1 hit.
    3.40.1280.10. 1 hit.
    HAMAPi MF_00605. TrmD.
    InterProi IPR029028. Alpha/beta_knot_MTases.
    IPR002649. tRNA_m1G_MeTrfase_bac.
    IPR023148. tRNA_m1G_MeTrfase_C.
    IPR029026. tRNA_m1G_MTases_N.
    IPR016009. tRNA_MeTrfase_TRMD/TRM10.
    [Graphical view ]
    Pfami PF01746. tRNA_m1G_MT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000386. tRNA_mtase. 1 hit.
    SUPFAMi SSF75217. SSF75217. 1 hit.
    TIGRFAMsi TIGR00088. trmD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition."
      Ahn H.J., Kim H.-W., Yoon H.-J., Lee B.I., Suh S.W., Yang J.K.
      EMBO J. 22:2593-2603(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, SUBUNIT.

    Entry informationi

    Entry nameiTRMD_HAEIN
    AccessioniPrimary (citable) accession number: P43912
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: July 9, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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