Skip Header

Contribute Send feedback
Read comments (?) or add your own

P43912 (TRMD_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:HI_0202
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer. Ref.2

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_0000060385

Regions

Region133 – 1386S-adenosyl-L-methionine binding HAMAP MF_00605

Sites

Active site1691Proton acceptor Potential
Binding site861S-adenosyl-L-methionine
Binding site1131S-adenosyl-L-methionine; via amide nitrogen

Secondary structure

1 ...................................... 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P43912 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: DEEF238159B1003D

FASTA24627,543
        10         20         30         40         50         60 
MWIGVISLFP EMFKAITEFG VTGRAVKHNL LKVECWNPRD FTFDKHKTVD DRPYGGGPGM 

        70         80         90        100        110        120 
LMMVQPLRDA IHTAKAAAGE GAKVIYLSPQ GRKLDQGGVT ELAQNQKLIL VCGRYEGIDE 

       130        140        150        160        170        180 
RLIQTEIDEE WSIGDYVLTG GELPAMTLID AVARFIPGVL GKQASAEEDS FADGLLDCPH 

       190        200        210        220        230        240 
YTRPEVLEGL TVPPVLMSGH HEEIRKWRLK QSLQRTWLRR PELLEGLALT DEQRKLLKEA 


QAEHNS

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42023 Genomic DNA. Translation: AAC21871.1.
PIRC64054.
RefSeqNP_438371.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UAJX-ray1.85A1-246[»]
1UAKX-ray2.05A1-246[»]
1UALX-ray1.80A1-246[»]
1UAMX-ray2.20A1-246[»]
3AXZX-ray2.25A1-246[»]
ProteinModelPortalP43912.
SMRP43912. Positions 1-246.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID951111.
GenomeReviewsGene locus HI_0202 in contig L42023_GR.
KEGGhin:HI0202.
PATRIC20188901. VBIHaeInf48452_0207.
TIGRHI_0202.

Phylogenomic databases

HOGENOMHBG285805.
OMAVCGRFEG.
PhylomeDBP43912.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycHINF71421:HI_0202-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_HAEIN
AccessionPrimary (citable) accession number: P43912
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents