Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (PA2943), Phospho-2-dehydro-3-deoxyheptonate aldolase (PA1750)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase 2 (aroQ2), 3-dehydroquinate dehydratase 1 (aroQ1)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60ShikimateUniRule annotation1
Active sitei64Proton acceptorUniRule annotation1
Binding sitei76NADPUniRule annotation1
Binding sitei85ShikimateUniRule annotation1
Binding sitei101ShikimateUniRule annotation1
Binding sitei214NADP; via carbonyl oxygenUniRule annotation1
Binding sitei216ShikimateUniRule annotation1
Binding sitei238NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 130NADPUniRule annotation5
Nucleotide bindingi150 – 155NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-25-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:PA0025
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0025

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360251 – 274Shikimate dehydrogenase (NADP(+))Add BLAST274

Proteomic databases

PaxDbiP43904

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0025

Structurei

3D structure databases

ProteinModelPortaliP43904
SMRiP43904
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237876
InParanoidiP43904
KOiK00014
OMAiFGNPIKH
PhylomeDBiP43904

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

P43904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRYCVFGNP IGHSKSPLIH RLFAEQTGEA LVYDAQLAPL DDFPGFARRF
60 70 80 90 100
FEQGKGANVT VPFKEEAYRL VDELSERATR AGAVNTLIRL ADGRLRGDNT
110 120 130 140 150
DGAGLLRDLT ANAGVELRGK RVLLLGAGGA VRGVLEPFLG ECPAELLIAN
160 170 180 190 200
RTARKAVDLA ERFADLGAVH GCGFAEVEGP FDLIVNGTSA SLAGDVPPLA
210 220 230 240 250
QSVIEPGRTV CYDMMYAKEP TAFNRWAAER GAARTLDGLG MLVEQAAEAF
260 270
FLWRGVRPAS APVLETLRRQ LATV
Length:274
Mass (Da):29,485
Last modified:December 8, 2000 - v3
Checksum:iF1B58750AFA9195E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116 – 117EL → DV in CAA59377 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85015 Genomic DNA Translation: CAA59377.1
AE004091 Genomic DNA Translation: AAG03415.1
PIRiG83641
RefSeqiNP_248715.1, NC_002516.2
WP_003111201.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG03415; AAG03415; PA0025
GeneIDi880894
KEGGipae:PA0025
PATRICifig|208964.12.peg.24

Similar proteinsi

Entry informationi

Entry nameiAROE_PSEAE
AccessioniPrimary (citable) accession number: P43904
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 8, 2000
Last modified: March 28, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health