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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (PA2943), Phospho-2-dehydro-3-deoxyheptonate aldolase (PA1750)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase 2 (aroQ2), 3-dehydroquinate dehydratase 1 (aroQ1)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601ShikimateUniRule annotation
Active sitei64 – 641Proton acceptorUniRule annotation
Binding sitei76 – 761NADPUniRule annotation
Binding sitei85 – 851ShikimateUniRule annotation
Binding sitei101 – 1011ShikimateUniRule annotation
Binding sitei214 – 2141NADP; via carbonyl oxygenUniRule annotation
Binding sitei216 – 2161ShikimateUniRule annotation
Binding sitei238 – 2381NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1305NADPUniRule annotation
Nucleotide bindingi150 – 1556NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:PA0025
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0025.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Shikimate dehydrogenase (NADP(+))PRO_0000136025Add
BLAST

Proteomic databases

PaxDbiP43904.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0025.

Structurei

3D structure databases

ProteinModelPortaliP43904.
SMRiP43904. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 163Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237876.
InParanoidiP43904.
KOiK00014.
OMAiFGNPIKH.
PhylomeDBiP43904.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P43904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRYCVFGNP IGHSKSPLIH RLFAEQTGEA LVYDAQLAPL DDFPGFARRF
60 70 80 90 100
FEQGKGANVT VPFKEEAYRL VDELSERATR AGAVNTLIRL ADGRLRGDNT
110 120 130 140 150
DGAGLLRDLT ANAGVELRGK RVLLLGAGGA VRGVLEPFLG ECPAELLIAN
160 170 180 190 200
RTARKAVDLA ERFADLGAVH GCGFAEVEGP FDLIVNGTSA SLAGDVPPLA
210 220 230 240 250
QSVIEPGRTV CYDMMYAKEP TAFNRWAAER GAARTLDGLG MLVEQAAEAF
260 270
FLWRGVRPAS APVLETLRRQ LATV
Length:274
Mass (Da):29,485
Last modified:December 8, 2000 - v3
Checksum:iF1B58750AFA9195E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti116 – 1172EL → DV in CAA59377 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85015 Genomic DNA. Translation: CAA59377.1.
AE004091 Genomic DNA. Translation: AAG03415.1.
PIRiG83641.
RefSeqiNP_248715.1. NC_002516.2.
WP_003111201.1. NZ_ASJY01000004.1.

Genome annotation databases

EnsemblBacteriaiAAG03415; AAG03415; PA0025.
GeneIDi880894.
KEGGipae:PA0025.
PATRICi19834244. VBIPseAer58763_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85015 Genomic DNA. Translation: CAA59377.1.
AE004091 Genomic DNA. Translation: AAG03415.1.
PIRiG83641.
RefSeqiNP_248715.1. NC_002516.2.
WP_003111201.1. NZ_ASJY01000004.1.

3D structure databases

ProteinModelPortaliP43904.
SMRiP43904. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0025.

Proteomic databases

PaxDbiP43904.

Protocols and materials databases

DNASUi880894.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03415; AAG03415; PA0025.
GeneIDi880894.
KEGGipae:PA0025.
PATRICi19834244. VBIPseAer58763_0024.

Organism-specific databases

PseudoCAPiPA0025.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237876.
InParanoidiP43904.
KOiK00014.
OMAiFGNPIKH.
PhylomeDBiP43904.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_PSEAE
AccessioniPrimary (citable) accession number: P43904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 8, 2000
Last modified: September 7, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.