P43898 (HEM6_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	PA0024

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_0000109910
Regions
	Region	49 – 58	10	Important for dimerization By similarity
	Region	109 – 111	3	Substrate binding By similarity
	Region	241 – 276	36	Important for dimerization By similarity
	Region	259 – 264	6	Substrate binding By similarity
Sites
	Active site	107	1	Proton donor By similarity
	Binding site	93	1	Substrate By similarity
	Site	176	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43898 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 240BD06FEA37EAE3	FASTA	305	34,806
	10 20 30 40 50 60 MTDRIAAVKT YLLDLQDRIC AALEAEDGKA RFAEDAWERP AGGGGRTRVI GDGALIEKGG 70 80 90 100 110 120 VNFSHVFGDS LPPSASAHRP ELAGRGFQAL GVSLVIHPEN PHVPTSHANV RFFCAEKEGE 130 140 150 160 170 180 EPVWWFGGGF DLTPYYAHEE DCVHWHRVAR DACAPFGADV YPRYKEWCDR YFHLKHRNEP 190 200 210 220 230 240 RGIGGLFFDD LNQWDFDTCF AFIRAIGDAY IDAYLPIVQR RKHTPFDERQ REFQAYRRGR 250 260 270 280 290 300 YVEFNLVFDR GTLFGLQSGG RTESILMSLP PQVRWGYDWK PEPGSEEARL TEYFLADRDW LAGQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and regulation of the Pseudomonas aeruginosa hemF gene encoding oxygen-dependent coproporphyrinogen III oxidase." Hungerer C., Troup B., Jahn D. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85015 Genomic DNA. Translation: CAA59376.1. AE004091 Genomic DNA. Translation: AAG03414.1.
PIR	S52924.
RefSeq	NP_248714.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P43898.
SMR	P43898. Positions 1-301.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880901.
GenomeReviews	Gene locus PA0024 in contig AE004091_GR.
KEGG	pae:PA0024.
PATRIC	19834242. VBIPseAer58763_0023.
Organism-specific databases
PseudoCAP	PA0024.
Phylogenomic databases
HOGENOM	HBG631180.
OMA	VKAYLLD.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	PAER208964:PA0024-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_PSEAE

Accession

Primary (citable) accession number: P43898

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program