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Protein

Elongation factor Ts, mitochondrial

Gene

TSFM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.UniRule annotation

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • translation elongation factor activity Source: GO_Central

GO - Biological processi

  • mitochondrial translational elongation Source: GO_Central
  • regulation of DNA-templated transcription, elongation Source: ProtInc
  • translational elongation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123297-MONOMER.
ReactomeiR-HSA-5389840. Mitochondrial translation elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Ts, mitochondrialUniRule annotation
Short name:
EF-TsUniRule annotation
Short name:
EF-TsMtUniRule annotation
Gene namesi
Name:TSFMUniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:12367. TSFM.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Combined oxidative phosphorylation deficiency 3 (COXPD3)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA mitochondrial disease resulting in severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes.
See also OMIM:610505
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_068973312R → W in COXPD3. 2 PublicationsCorresponds to variant rs121909485dbSNPEnsembl.1

Keywords - Diseasei

Cardiomyopathy, Disease mutation

Organism-specific databases

DisGeNETi10102.
MalaCardsiTSFM.
MIMi610505. phenotype.
OpenTargetsiENSG00000123297.
Orphaneti168566. Fatal mitochondrial disease due to combined oxidative phosphorylation deficiency 3.
PharmGKBiPA37037.

Polymorphism and mutation databases

BioMutaiTSFM.
DMDMi12644268.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45MitochondrionUniRule annotationAdd BLAST45
ChainiPRO_000000746846 – 325Elongation factor Ts, mitochondrialAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76N6-succinyllysineBy similarity1
Modified residuei133N6-succinyllysineBy similarity1
Modified residuei192N6-succinyllysineBy similarity1
Modified residuei270PhosphoserineCombined sources1
Modified residuei324PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP43897.
MaxQBiP43897.
PeptideAtlasiP43897.
PRIDEiP43897.

PTM databases

iPTMnetiP43897.
PhosphoSitePlusiP43897.

Expressioni

Tissue specificityi

Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.

Gene expression databases

BgeeiENSG00000123297.
CleanExiHS_TSFM.
ExpressionAtlasiP43897. baseline and differential.
GenevisibleiP43897. HS.

Organism-specific databases

HPAiHPA006204.

Interactioni

Protein-protein interaction databases

BioGridi115409. 26 interactors.
IntActiP43897. 12 interactors.
MINTiMINT-3297563.

Structurei

Secondary structure

1325
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi48 – 57Combined sources10
Helixi61 – 71Combined sources11
Helixi75 – 89Combined sources15
Beta strandi92 – 94Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CP9NMR-A45-95[»]
ProteinModelPortaliP43897.
SMRiP43897.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43897.

Family & Domainsi

Sequence similaritiesi

Belongs to the EF-Ts family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000016293.
HOGENOMiHOG000220988.
HOVERGENiHBG005571.
InParanoidiP43897.
KOiK02357.
OMAiLVICETS.
OrthoDBiEOG091G0E11.
PhylomeDBiP43897.
TreeFamiTF314154.

Family and domain databases

Gene3Di3.30.479.20. 2 hits.
HAMAPiMF_00050. EF_Ts. 1 hit.
InterProiIPR001816. Transl_elong_EFTs/EF1B.
IPR014039. Transl_elong_EFTs/EF1B_dimer.
IPR018101. Transl_elong_Ts_CS.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR11741. PTHR11741. 1 hit.
PfamiPF00889. EF_TS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54713. SSF54713. 2 hits.
PROSITEiPS01126. EF_TS_1. 1 hit.
PS01127. EF_TS_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P43897-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLRSLRVF LVARTGSYPA GSLLRQSPQP RHTFYAGPRL SASASSKELL
60 70 80 90 100
MKLRRKTGYS FVNCKKALET CGGDLKQAEI WLHKEAQKEG WSKAAKLQGR
110 120 130 140 150
KTKEGLIGLL QEGNTTVLVE VNCETDFVSR NLKFQLLVQQ VALGTMMHCQ
160 170 180 190 200
TLKDQPSAYS KGFLNSSELS GLPAGPDREG SLKDQLALAI GKLGENMILK
210 220 230 240 250
RAAWVKVPSG FYVGSYVHGA MQSPSLHKLV LGKYGALVIC ETSEQKTNLE
260 270 280 290 300
DVGRRLGQHV VGMAPLSVGS LDDEPGGEAE TKMLSQPYLL DPSITLGQYV
310 320
QPQGVSVVDF VRFECGEGEE AAETE
Length:325
Mass (Da):35,391
Last modified:January 11, 2001 - v2
Checksum:i671645764A9CB31C
GO
Isoform 2 (identifier: P43897-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     161-161: K → KVQWLTPVNLALWEAEAGGSLE

Note: No experimental confirmation available.
Show »
Length:346
Mass (Da):37,656
Checksum:i15E85F6DF0FE8714
GO
Isoform 3 (identifier: P43897-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     162-167: GFLNSS → ENWEKT
     168-325: Missing.

Note: No experimental confirmation available.
Show »
Length:167
Mass (Da):18,748
Checksum:i32E5D247199AF171
GO
Isoform 4 (identifier: P43897-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-215: KLGENMILKRAAWVKVPSGFYVGS → LPFQIGAVSELQLPESNFLQNTSS
     216-325: Missing.

Note: No experimental confirmation available.
Show »
Length:215
Mass (Da):23,557
Checksum:i018E9C1CD7458EBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32 – 34HTF → ARV in AAC37577 (PubMed:7615523).Curated3
Sequence conflicti196N → T in AAH93068 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_068973312R → W in COXPD3. 2 PublicationsCorresponds to variant rs121909485dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001364161K → KVQWLTPVNLALWEAEAGGS LE in isoform 2. 1 Publication1
Alternative sequenceiVSP_043517162 – 167GFLNSS → ENWEKT in isoform 3. 1 Publication6
Alternative sequenceiVSP_043518168 – 325Missing in isoform 3. 1 PublicationAdd BLAST158
Alternative sequenceiVSP_045283192 – 215KLGEN…FYVGS → LPFQIGAVSELQLPESNFLQ NTSS in isoform 4. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_045284216 – 325Missing in isoform 4. 1 PublicationAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110399 mRNA. Translation: AAD20224.1.
AK304621 mRNA. Translation: BAG65403.1.
AK308981 mRNA. No translation available.
AC025165 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97075.1.
BC022862 mRNA. Translation: AAH22862.1.
BC093068 mRNA. Translation: AAH93068.1.
L37936 mRNA. Translation: AAC37577.1.
CCDSiCCDS53809.1. [P43897-2]
CCDS53810.1. [P43897-4]
CCDS53811.1. [P43897-3]
CCDS8958.2. [P43897-1]
PIRiI84606.
RefSeqiNP_001166166.1. NM_001172695.1. [P43897-3]
NP_001166167.1. NM_001172696.1. [P43897-2]
NP_001166168.1. NM_001172697.1. [P43897-4]
NP_005717.3. NM_005726.5. [P43897-1]
UniGeneiHs.632704.

Genome annotation databases

EnsembliENST00000323833; ENSP00000313877; ENSG00000123297. [P43897-2]
ENST00000454289; ENSP00000388330; ENSG00000123297. [P43897-1]
ENST00000540550; ENSP00000440987; ENSG00000123297. [P43897-3]
ENST00000543727; ENSP00000439342; ENSG00000123297. [P43897-4]
GeneIDi10102.
KEGGihsa:10102.
UCSCiuc001sqh.4. human. [P43897-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110399 mRNA. Translation: AAD20224.1.
AK304621 mRNA. Translation: BAG65403.1.
AK308981 mRNA. No translation available.
AC025165 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW97075.1.
BC022862 mRNA. Translation: AAH22862.1.
BC093068 mRNA. Translation: AAH93068.1.
L37936 mRNA. Translation: AAC37577.1.
CCDSiCCDS53809.1. [P43897-2]
CCDS53810.1. [P43897-4]
CCDS53811.1. [P43897-3]
CCDS8958.2. [P43897-1]
PIRiI84606.
RefSeqiNP_001166166.1. NM_001172695.1. [P43897-3]
NP_001166167.1. NM_001172696.1. [P43897-2]
NP_001166168.1. NM_001172697.1. [P43897-4]
NP_005717.3. NM_005726.5. [P43897-1]
UniGeneiHs.632704.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CP9NMR-A45-95[»]
ProteinModelPortaliP43897.
SMRiP43897.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115409. 26 interactors.
IntActiP43897. 12 interactors.
MINTiMINT-3297563.

PTM databases

iPTMnetiP43897.
PhosphoSitePlusiP43897.

Polymorphism and mutation databases

BioMutaiTSFM.
DMDMi12644268.

Proteomic databases

EPDiP43897.
MaxQBiP43897.
PeptideAtlasiP43897.
PRIDEiP43897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323833; ENSP00000313877; ENSG00000123297. [P43897-2]
ENST00000454289; ENSP00000388330; ENSG00000123297. [P43897-1]
ENST00000540550; ENSP00000440987; ENSG00000123297. [P43897-3]
ENST00000543727; ENSP00000439342; ENSG00000123297. [P43897-4]
GeneIDi10102.
KEGGihsa:10102.
UCSCiuc001sqh.4. human. [P43897-1]

Organism-specific databases

CTDi10102.
DisGeNETi10102.
GeneCardsiTSFM.
HGNCiHGNC:12367. TSFM.
HPAiHPA006204.
MalaCardsiTSFM.
MIMi604723. gene.
610505. phenotype.
neXtProtiNX_P43897.
OpenTargetsiENSG00000123297.
Orphaneti168566. Fatal mitochondrial disease due to combined oxidative phosphorylation deficiency 3.
PharmGKBiPA37037.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000016293.
HOGENOMiHOG000220988.
HOVERGENiHBG005571.
InParanoidiP43897.
KOiK02357.
OMAiLVICETS.
OrthoDBiEOG091G0E11.
PhylomeDBiP43897.
TreeFamiTF314154.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123297-MONOMER.
ReactomeiR-HSA-5389840. Mitochondrial translation elongation.

Miscellaneous databases

EvolutionaryTraceiP43897.
GeneWikiiTSFM.
GenomeRNAii10102.
PROiP43897.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123297.
CleanExiHS_TSFM.
ExpressionAtlasiP43897. baseline and differential.
GenevisibleiP43897. HS.

Family and domain databases

Gene3Di3.30.479.20. 2 hits.
HAMAPiMF_00050. EF_Ts. 1 hit.
InterProiIPR001816. Transl_elong_EFTs/EF1B.
IPR014039. Transl_elong_EFTs/EF1B_dimer.
IPR018101. Transl_elong_Ts_CS.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR11741. PTHR11741. 1 hit.
PfamiPF00889. EF_TS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54713. SSF54713. 2 hits.
PROSITEiPS01126. EF_TS_1. 1 hit.
PS01127. EF_TS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFTS_HUMAN
AccessioniPrimary (citable) accession number: P43897
Secondary accession number(s): B4E391
, F5H2T7, Q561V7, Q8TBC2, Q9UQK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.