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Protein

Elongation factor Ts

Gene

tsf

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-892-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Ts
Short name:
EF-Ts
Gene namesi
Name:tsf
Ordered Locus Names:TTHA0860
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Elongation factor TsPRO_0000161222Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of two EF-Ts.EF-Tu dimer complexes.

Protein-protein interaction databases

DIPiDIP-6078N.
IntActiP43895. 1 interaction.
STRINGi300852.TTHA0860.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Helixi18 – 2710Combined sources
Turni28 – 303Combined sources
Helixi32 – 5019Combined sources
Beta strandi57 – 648Combined sources
Beta strandi68 – 7912Combined sources
Helixi81 – 855Combined sources
Helixi87 – 10317Combined sources
Beta strandi106 – 1094Combined sources
Helixi110 – 1123Combined sources
Helixi115 – 13016Combined sources
Turni131 – 1333Combined sources
Helixi136 – 15419Combined sources
Helixi156 – 1583Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi164 – 1685Combined sources
Helixi169 – 18012Combined sources
Beta strandi185 – 1939Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIPX-ray3.00C/D/G/H1-196[»]
1TFEX-ray1.70A55-196[»]
ProteinModelPortaliP43895.
SMRiP43895. Positions 2-196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43895.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 834Involved in Mg(2+) ion dislocation from EF-TuBy similarity

Sequence similaritiesi

Belongs to the EF-Ts family.Curated

Phylogenomic databases

eggNOGiENOG4105CU7. Bacteria.
COG0264. LUCA.
HOGENOMiHOG000220986.
KOiK02357.
OMAiQIYIQAA.
OrthoDBiEOG66B42N.
PhylomeDBiP43895.

Family and domain databases

Gene3Di3.30.479.20. 2 hits.
HAMAPiMF_00050. EF_Ts.
InterProiIPR001816. Transl_elong_EFTs/EF1B.
IPR014039. Transl_elong_EFTs/EF1B_dimer.
IPR018101. Transl_elong_Ts_CS.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR11741. PTHR11741. 1 hit.
PfamiPF00889. EF_TS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54713. SSF54713. 1 hit.
TIGRFAMsiTIGR00116. tsf. 1 hit.
PROSITEiPS01126. EF_TS_1. 1 hit.
PS01127. EF_TS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQMELIKKL REATGAGMMD VKRALEDAGW DEEKAVQLLR ERGAMKAAKK
60 70 80 90 100
ADREAREGII GHYIHHNQRV GVLVELNCET DFVARNELFQ NLAKDLAMHI
110 120 130 140 150
AMMNPRYVSA EEIPAEELEK ERQIYIQAAL NEGKPQQIAE KIAEGRLKKY
160 170 180 190
LEEVVLLEQP FVKDDKVKVK ELIQQAIAKI GENIVVRRFC RFELGA
Length:196
Mass (Da):22,413
Last modified:November 1, 1995 - v1
Checksum:i36A771F686BBB0DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83598 Genomic DNA. Translation: CAA58578.1.
AP008226 Genomic DNA. Translation: BAD70683.1.
RefSeqiWP_011228249.1. NC_006461.1.
YP_144126.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70683; BAD70683; BAD70683.
GeneIDi3170120.
KEGGittj:TTHA0860.
PATRICi23956702. VBITheThe93045_0854.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83598 Genomic DNA. Translation: CAA58578.1.
AP008226 Genomic DNA. Translation: BAD70683.1.
RefSeqiWP_011228249.1. NC_006461.1.
YP_144126.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIPX-ray3.00C/D/G/H1-196[»]
1TFEX-ray1.70A55-196[»]
ProteinModelPortaliP43895.
SMRiP43895. Positions 2-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6078N.
IntActiP43895. 1 interaction.
STRINGi300852.TTHA0860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70683; BAD70683; BAD70683.
GeneIDi3170120.
KEGGittj:TTHA0860.
PATRICi23956702. VBITheThe93045_0854.

Phylogenomic databases

eggNOGiENOG4105CU7. Bacteria.
COG0264. LUCA.
HOGENOMiHOG000220986.
KOiK02357.
OMAiQIYIQAA.
OrthoDBiEOG66B42N.
PhylomeDBiP43895.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-892-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP43895.

Family and domain databases

Gene3Di3.30.479.20. 2 hits.
HAMAPiMF_00050. EF_Ts.
InterProiIPR001816. Transl_elong_EFTs/EF1B.
IPR014039. Transl_elong_EFTs/EF1B_dimer.
IPR018101. Transl_elong_Ts_CS.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR11741. PTHR11741. 1 hit.
PfamiPF00889. EF_TS. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54713. SSF54713. 1 hit.
TIGRFAMsiTIGR00116. tsf. 1 hit.
PROSITEiPS01126. EF_TS_1. 1 hit.
PS01127. EF_TS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Elongation factor Ts from Thermus thermophilus -- overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu."
    Blank J., Nock S., Kreutzer R., Sprinzl M.
    Eur. J. Biochem. 236:222-227(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Crystal structure of the EF-Tu.EF-Ts complex from Thermus thermophilus."
    Wang Y., Jiang Y., Meyering-Voss M., Sprinzl M., Sigler P.B.
    Nat. Struct. Biol. 4:650-656(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF COMPLEX WITH EF-TU.

Entry informationi

Entry nameiEFTS_THET8
AccessioniPrimary (citable) accession number: P43895
Secondary accession number(s): Q5SJZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 11, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.