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Protein

Uridylate kinase

Gene

pyrH

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.1 Publication

Catalytic activityi

ATP + UMP = ADP + UDP.

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.1 Publication

Kineticsi

  1. KM=1.57 mM for ATP (in the absence of GTP)1 Publication
  2. KM=0.46 mM for ATP (in the presence of GTP)1 Publication
  3. KM=40 µM for UMP1 Publication
  1. Vmax=57 µmol/min/mg enzyme1 Publication

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54UMP; via amide nitrogenBy similarity1
Binding sitei55ATP; via amide nitrogenBy similarity1
Binding sitei59ATPBy similarity1
Binding sitei74UMPBy similarity1
Binding sitei162ATPBy similarity1
Binding sitei168ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei171ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15ATPBy similarity4
Nucleotide bindingi135 – 142UMPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP43890.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinase (EC:2.7.4.22)
Short name:
UK
Alternative name(s):
Uridine monophosphate kinase
Short name:
UMP kinase
Short name:
UMPK
Gene namesi
Name:pyrH
Synonyms:smbA
Ordered Locus Names:HI_1065
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001438471 – 237Uridylate kinaseAdd BLAST237

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI1065.

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 13Combined sources7
Helixi15 – 18Combined sources4
Beta strandi23 – 25Combined sources3
Helixi28 – 42Combined sources15
Turni43 – 45Combined sources3
Beta strandi47 – 52Combined sources6
Turni55 – 57Combined sources3
Helixi61 – 65Combined sources5
Helixi70 – 94Combined sources25
Beta strandi99 – 105Combined sources7
Turni108 – 110Combined sources3
Beta strandi111 – 113Combined sources3
Helixi116 – 124Combined sources9
Beta strandi128 – 133Combined sources6
Helixi142 – 152Combined sources11
Beta strandi156 – 166Combined sources11
Beta strandi182 – 184Combined sources3
Helixi186 – 191Combined sources6
Helixi199 – 208Combined sources10
Beta strandi212 – 216Combined sources5
Helixi222 – 227Combined sources6
Beta strandi232 – 236Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A1FX-ray2.10A/B/C/D/E/F2-237[»]
ProteinModelPortaliP43890.
SMRiP43890.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43890.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 25Involved in allosteric activation by GTPSequence analysis6

Sequence similaritiesi

Belongs to the UMP kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
KOiK09903.
OMAiKGLKVMD.
PhylomeDBiP43890.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P43890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPIYKRIL LKLSGEALQG EDGLGIDPAI LDRMAVEIKE LVEMGVEVSV
60 70 80 90 100
VLGGGNLFRG AKLAKAGMNR VVGDHMGMLA TVMNGLAMRD SLFRADVNAK
110 120 130 140 150
LMSAFQLNGI CDTYNWSEAI KMLREKRVVI FSAGTGNPFF TTDSTACLRG
160 170 180 190 200
IEIEADVVLK ATKVDGVYDC DPAKNPDAKL YKNLSYAEVI DKELKVMDLS
210 220 230
AFTLARDHGM PIRVFNMGKP GALRQVVTGT EEGTTIC
Length:237
Mass (Da):25,726
Last modified:November 1, 1995 - v1
Checksum:iFDB0D6CE2CBCDCE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22719.1.
PIRiH64180.
RefSeqiNP_439223.1. NC_000907.1.
WP_005693391.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22719; AAC22719; HI_1065.
GeneIDi950042.
KEGGihin:HI1065.
PATRICi20190793. VBIHaeInf48452_1109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22719.1.
PIRiH64180.
RefSeqiNP_439223.1. NC_000907.1.
WP_005693391.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A1FX-ray2.10A/B/C/D/E/F2-237[»]
ProteinModelPortaliP43890.
SMRiP43890.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1065.

Protocols and materials databases

DNASUi950042.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22719; AAC22719; HI_1065.
GeneIDi950042.
KEGGihin:HI1065.
PATRICi20190793. VBIHaeInf48452_1109.

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
KOiK09903.
OMAiKGLKVMD.
PhylomeDBiP43890.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
SABIO-RKP43890.

Miscellaneous databases

EvolutionaryTraceiP43890.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_HAEIN
AccessioniPrimary (citable) accession number: P43890
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.