P43884 (PLIN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Perilipin-1

Alternative name(s):
Lipid droplet-associated protein

Gene names

Name:	Plin1
Synonyms:	Peri, Plin

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
Subunit structure	Interacts with ABHD5.
Subcellular location	Lipid droplet. Note: Lipid droplet surface-associated.
Tissue specificity	Adipocytes.
Post-translational modification	Major cAMP-dependent protein kinase substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL. When unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. Ref.2 The N-terminus is blocked.
Sequence similarities	Belongs to the perilipin family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Lipid droplet
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	lipid particle Inferred from direct assay PubMed 17175194Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P43884-1)

Also known as: PERIA;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P43884-2)

Also known as: PERIB;

The sequence of this isoform differs from the canonical sequence as follows:
     407-422: LPRLSLMEPESEFQDI → VSPAPGPPSDSQGRFD
     423-517: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Perilipin-1		PRO_0000099886
Regions
	Compositional bias	308 – 319	12	Poly-Glu
Amino acid modifications
	Modified residue	411	1	Phosphoserine By similarity
	Modified residue	460	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	407 – 422	16	LPRLS…EFQDI → VSPAPGPPSDSQGRFD in isoform B.		VSP_004662
	Alternative sequence	423 – 517	95	Missing in isoform B.		VSP_004663

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (PERIA) [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1041F76DC2F55A25	FASTA	517	55,614
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEY LLPPDKVESA PSSGRQKTQK APKAKPSLLR RVSTLANTLS RHTMQTTARA 250 260 270 280 290 300 LKRGHSLAMW IPGVAPLSSL AQWGASAAMQ VVSRRQSEVR VPWLHNLAAS KDENHEDQTD 310 320 330 340 350 360 TEGEETDEEE EEEESEAEEN VLREVTALPT PLGFLGGVVH TVQKTLQNTI SAVTWAPAAV 370 380 390 400 410 420 LGTVGRILHL TPAQAVSSTK GRAMSLSDAL KGVTDNVVDT VVHYVPLPRL SLMEPESEFQ 430 440 450 460 470 480 DIDNPPAEVE RKGSGSRPAS PESTARPGQP RAACAVRGLS APSCPDLDDK TETSARPGLL 490 500 510 AMPREKPARR VSDSFFRPSV MEPILGRTQY SQLRKKS
	Isoform B (PERIB) [UniParc]. Checksum: B07043C3ABB4758B	FASTA	422	45,080
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEY LLPPDKVESA PSSGRQKTQK APKAKPSLLR RVSTLANTLS RHTMQTTARA 250 260 270 280 290 300 LKRGHSLAMW IPGVAPLSSL AQWGASAAMQ VVSRRQSEVR VPWLHNLAAS KDENHEDQTD 310 320 330 340 350 360 TEGEETDEEE EEEESEAEEN VLREVTALPT PLGFLGGVVH TVQKTLQNTI SAVTWAPAAV 370 380 390 400 410 420 LGTVGRILHL TPAQAVSSTK GRAMSLSDAL KGVTDNVVDT VVHYVPVSPA PGPPSDSQGR FD

References

[1]	"Isolation of cDNAs for perilipins A and B: sequence and expression of lipid droplet-associated proteins of adipocytes." Greenberg A.S., Egan J.J., Wek S.A., Moos M.C. Jr., Londos C., Kimmel A.R. Proc. Natl. Acad. Sci. U.S.A. 90:12035-12039(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Adipocyte.
[2]	"Dephosphorylation of perilipin by protein phosphatases present in rat adipocytes." Clifford G.M., McCormick D.K., Londos C., Vernon R.G., Yeaman S.J. FEBS Lett. 435:125-129(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Fat, wonderful fat - Issue 10 of May 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26043 mRNA. Translation: AAA41830.1. L26044 mRNA. Translation: AAA41831.1.
IPI	IPI00230811. IPI00947731.
PIR	A49413.
RefSeq	NP_037226.1. NM_013094.1.
UniGene	Rn.9737.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000020559.
PTM databases
PhosphoSite	P43884.
Proteomic databases
PRIDE	P43884.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25629.
KEGG	rno:25629.
UCSC	RGD:3351. rat.
Organism-specific databases
CTD	5346.
RGD	3351. Plin1.
Phylogenomic databases
eggNOG	NOG75006.
HOGENOM	HOG000261608.
InParanoid	P43884.
KO	K08768.
Enzyme and pathway databases
Reactome	REACT_113568. Metabolism.
Gene expression databases
ArrayExpress	P43884.
Genevestigator	P43884.
GermOnline	ENSRNOG00000015086. Rattus norvegicus.
Family and domain databases
InterPro	IPR004279. Perilipin. [Graphical view]
PANTHER	PTHR14024. PTHR14024. 1 hit.
Pfam	PF03036. Perilipin. 1 hit. [Graphical view]
PIRSF	PIRSF036881. PAT. 1 hit.
ProtoNet	Search...
Other
NextBio	607427.

Entry information

Entry name

PLIN1_RAT

Accession

Primary (citable) accession number: P43884

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program