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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ShikimateUniRule annotation
Active sitei65 – 651Proton acceptorUniRule annotation
Binding sitei86 – 861ShikimateUniRule annotation
Binding sitei102 – 1021ShikimateUniRule annotation
Binding sitei189 – 1891NADP1 Publication
Binding sitei213 – 2131NADP; via carbonyl oxygenUniRule annotation
Binding sitei215 – 2151ShikimateUniRule annotation
Binding sitei238 – 2381NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1305NADPUniRule annotation1 Publication
Nucleotide bindingi149 – 1546NADPUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.25. 2529.
SABIO-RKP43876.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Shikimate dehydrogenase (NADP(+))PRO_0000136006Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi71421.HI0655.

Structurei

Secondary structure

1
272
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109Combined sources
Helixi16 – 2611Combined sources
Beta strandi31 – 377Combined sources
Turni40 – 423Combined sources
Helixi43 – 5210Combined sources
Beta strandi57 – 604Combined sources
Helixi65 – 717Combined sources
Beta strandi73 – 753Combined sources
Helixi77 – 826Combined sources
Beta strandi86 – 905Combined sources
Beta strandi92 – 943Combined sources
Beta strandi96 – 994Combined sources
Helixi102 – 11211Combined sources
Beta strandi121 – 1255Combined sources
Helixi129 – 1324Combined sources
Helixi135 – 1406Combined sources
Beta strandi144 – 1518Combined sources
Helixi152 – 16211Combined sources
Helixi163 – 1653Combined sources
Beta strandi168 – 1725Combined sources
Helixi173 – 1753Combined sources
Beta strandi182 – 1865Combined sources
Helixi201 – 2066Combined sources
Beta strandi210 – 2134Combined sources
Beta strandi217 – 2193Combined sources
Helixi222 – 2298Combined sources
Helixi239 – 25416Combined sources
Helixi260 – 27112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P74X-ray2.40A/B1-272[»]
1P77X-ray1.95A1-272[»]
ProteinModelPortaliP43876.
SMRiP43876. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43876.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 163Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiQPYGNIQ.
PhylomeDBiP43876.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P43876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLYAVWGNP IAQSKSPLIQ NKLAAQTHQT MEYIAKLGDL DAFEQQLLAF
60 70 80 90 100
FEEGAKGCNI TSPFKERAYQ LADEYSQRAK LAEACNTLKK LDDGKLYADN
110 120 130 140 150
TDGIGLVTDL QRLNWLRPNQ HVLILGAGGA TKGVLLPLLQ AQQNIVLANR
160 170 180 190 200
TFSKTKELAE RFQPYGNIQA VSMDSIPLQT YDLVINATSA GLSGGTASVD
210 220 230 240 250
AEILKLGSAF YDMQYAKGTD TPFIALCKSL GLTNVSDGFG MLVAQAAHSF
260 270
HLWRGVMPDF VSVYEQLKKA ML
Length:272
Mass (Da):29,760
Last modified:November 1, 1995 - v1
Checksum:i3B7A92AF22D4470B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22314.1.
PIRiH64084.
RefSeqiNP_438815.1. NC_000907.1.
WP_005694487.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22314; AAC22314; HI_0655.
GeneIDi949697.
KEGGihin:HI0655.
PATRICi20189925. VBIHaeInf48452_0684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22314.1.
PIRiH64084.
RefSeqiNP_438815.1. NC_000907.1.
WP_005694487.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P74X-ray2.40A/B1-272[»]
1P77X-ray1.95A1-272[»]
ProteinModelPortaliP43876.
SMRiP43876. Positions 1-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22314; AAC22314; HI_0655.
GeneIDi949697.
KEGGihin:HI0655.
PATRICi20189925. VBIHaeInf48452_0684.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiQPYGNIQ.
PhylomeDBiP43876.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BRENDAi1.1.1.25. 2529.
SABIO-RKP43876.

Miscellaneous databases

EvolutionaryTraceiP43876.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_HAEIN
AccessioniPrimary (citable) accession number: P43876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.