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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ShikimateUniRule annotation1
Active sitei65Proton acceptorUniRule annotation1
Binding sitei86ShikimateUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei189NADP1 Publication1
Binding sitei213NADP; via carbonyl oxygenUniRule annotation1
Binding sitei215ShikimateUniRule annotation1
Binding sitei238NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 130NADPUniRule annotation1 Publication5
Nucleotide bindingi149 – 154NADPUniRule annotation1 Publication6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.25. 2529.
SABIO-RKP43876.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360061 – 272Shikimate dehydrogenase (NADP(+))Add BLAST272

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi71421.HI0655.

Structurei

Secondary structure

1272
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi16 – 26Combined sources11
Beta strandi31 – 37Combined sources7
Turni40 – 42Combined sources3
Helixi43 – 52Combined sources10
Beta strandi57 – 60Combined sources4
Helixi65 – 71Combined sources7
Beta strandi73 – 75Combined sources3
Helixi77 – 82Combined sources6
Beta strandi86 – 90Combined sources5
Beta strandi92 – 94Combined sources3
Beta strandi96 – 99Combined sources4
Helixi102 – 112Combined sources11
Beta strandi121 – 125Combined sources5
Helixi129 – 132Combined sources4
Helixi135 – 140Combined sources6
Beta strandi144 – 151Combined sources8
Helixi152 – 162Combined sources11
Helixi163 – 165Combined sources3
Beta strandi168 – 172Combined sources5
Helixi173 – 175Combined sources3
Beta strandi182 – 186Combined sources5
Helixi201 – 206Combined sources6
Beta strandi210 – 213Combined sources4
Beta strandi217 – 219Combined sources3
Helixi222 – 229Combined sources8
Helixi239 – 254Combined sources16
Helixi260 – 271Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P74X-ray2.40A/B1-272[»]
1P77X-ray1.95A1-272[»]
ProteinModelPortaliP43876.
SMRiP43876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43876.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiQPYGNIQ.
PhylomeDBiP43876.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P43876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLYAVWGNP IAQSKSPLIQ NKLAAQTHQT MEYIAKLGDL DAFEQQLLAF
60 70 80 90 100
FEEGAKGCNI TSPFKERAYQ LADEYSQRAK LAEACNTLKK LDDGKLYADN
110 120 130 140 150
TDGIGLVTDL QRLNWLRPNQ HVLILGAGGA TKGVLLPLLQ AQQNIVLANR
160 170 180 190 200
TFSKTKELAE RFQPYGNIQA VSMDSIPLQT YDLVINATSA GLSGGTASVD
210 220 230 240 250
AEILKLGSAF YDMQYAKGTD TPFIALCKSL GLTNVSDGFG MLVAQAAHSF
260 270
HLWRGVMPDF VSVYEQLKKA ML
Length:272
Mass (Da):29,760
Last modified:November 1, 1995 - v1
Checksum:i3B7A92AF22D4470B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22314.1.
PIRiH64084.
RefSeqiNP_438815.1. NC_000907.1.
WP_005694487.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22314; AAC22314; HI_0655.
GeneIDi949697.
KEGGihin:HI0655.
PATRICi20189925. VBIHaeInf48452_0684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22314.1.
PIRiH64084.
RefSeqiNP_438815.1. NC_000907.1.
WP_005694487.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P74X-ray2.40A/B1-272[»]
1P77X-ray1.95A1-272[»]
ProteinModelPortaliP43876.
SMRiP43876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22314; AAC22314; HI_0655.
GeneIDi949697.
KEGGihin:HI0655.
PATRICi20189925. VBIHaeInf48452_0684.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiQPYGNIQ.
PhylomeDBiP43876.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BRENDAi1.1.1.25. 2529.
SABIO-RKP43876.

Miscellaneous databases

EvolutionaryTraceiP43876.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_HAEIN
AccessioniPrimary (citable) accession number: P43876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.