P43871 (MTH3_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HindIII
Short name=M.HindIII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase HindIII

Gene names

Name:	hindIIIM
Ordered Locus Names:	HI_1392

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence AAGCTT, causes specific methylation on A-1 on both strands, and protects the DNA from cleavage by the HindIII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Modification methylase HindIII		PRO_0000087964

Sequences

	Sequence		Length	Mass (Da)
	P43871 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 954E04023326E061	FASTA	309	35,550
	10 20 30 40 50 60 MIDCIYNSDS IFEIKKLDSN SIHAIISDIP YGIDYDDWDI LHSNTNSALG GTSSAQHKTS 70 80 90 100 110 120 LFKRRGKPLN GWSEADKKRP QEYQEWVESW SNEWFRVLKS GSSVFVFAGR QFAHRVVVAF 130 140 150 160 170 180 ENSGFTFKDM LSWEKDKAPH RAQRISCVFE RRGDIANTNK WVGWRVANLR PLFEPILWFQ 190 200 210 220 230 240 KPYKTGSTLA DNLIKHEVGA WNENSLTHWN IQQGALNHSN ILKVRITSED KGYHVAQKPL 250 260 270 280 290 300 NLMKLLIDLV TKEEQIVLDP FAGSGTTLLA AKELNRHFIG YEKNNGIYNI AVNRLGIEKN NCFYNKEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, analysis and expression of the HindIII R-M-encoding genes." Nwankwo D.O., Moran L.S., Slatko B.E., Waite-Rees P.A., Dorner L.F., Benner J.S., Wilson G.G. Gene 150:75-80(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L15391 Genomic DNA. Translation: AAA61959.1. L42023 Genomic DNA. Translation: AAC23039.1.
RefSeq	NP_439546.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43871.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1392.
Protein family/group databases
REBASE	3428. M.HindIII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC23039; AAC23039; HI_1392.
GeneID	950302.
KEGG	hin:HI1392.
PATRIC	20191481. VBIHaeInf48452_1451.
Phylogenomic databases
eggNOG	COG0863.
KO	K00571.
OMA	RAQRISC.
ProtClustDB	CLSK892404.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH3_HAEIN

Accession

Primary (citable) accession number: P43871

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program