Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43871

- MTH3_HAEIN

UniProt

P43871 - MTH3_HAEIN

Protein

Modification methylase HindIII

Gene

hindIIIM

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence AAGCTT, causes specific methylation on A-1 on both strands, and protects the DNA from cleavage by the HindIII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3428. M.HindIII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HindIII (EC:2.1.1.72)
    Short name:
    M.HindIII
    Alternative name(s):
    Adenine-specific methyltransferase HindIII
    Gene namesi
    Name:hindIIIM
    Ordered Locus Names:HI_1392
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Modification methylase HindIIIPRO_0000087964Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1392.

    Structurei

    3D structure databases

    ProteinModelPortaliP43871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0863.
    KOiK00571.
    OMAiNENSLTH.
    OrthoDBiEOG6FV84T.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P43871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDCIYNSDS IFEIKKLDSN SIHAIISDIP YGIDYDDWDI LHSNTNSALG    50
    GTSSAQHKTS LFKRRGKPLN GWSEADKKRP QEYQEWVESW SNEWFRVLKS 100
    GSSVFVFAGR QFAHRVVVAF ENSGFTFKDM LSWEKDKAPH RAQRISCVFE 150
    RRGDIANTNK WVGWRVANLR PLFEPILWFQ KPYKTGSTLA DNLIKHEVGA 200
    WNENSLTHWN IQQGALNHSN ILKVRITSED KGYHVAQKPL NLMKLLIDLV 250
    TKEEQIVLDP FAGSGTTLLA AKELNRHFIG YEKNNGIYNI AVNRLGIEKN 300
    NCFYNKEKK 309
    Length:309
    Mass (Da):35,550
    Last modified:November 1, 1995 - v1
    Checksum:i954E04023326E061
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15391 Genomic DNA. Translation: AAA61959.1.
    L42023 Genomic DNA. Translation: AAC23039.1.
    RefSeqiNP_439546.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC23039; AAC23039; HI_1392.
    GeneIDi950302.
    KEGGihin:HI1392.
    PATRICi20191481. VBIHaeInf48452_1451.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15391 Genomic DNA. Translation: AAA61959.1 .
    L42023 Genomic DNA. Translation: AAC23039.1 .
    RefSeqi NP_439546.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P43871.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1392.

    Protein family/group databases

    REBASEi 3428. M.HindIII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC23039 ; AAC23039 ; HI_1392 .
    GeneIDi 950302.
    KEGGi hin:HI1392.
    PATRICi 20191481. VBIHaeInf48452_1451.

    Phylogenomic databases

    eggNOGi COG0863.
    KOi K00571.
    OMAi NENSLTH.
    OrthoDBi EOG6FV84T.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, analysis and expression of the HindIII R-M-encoding genes."
      Nwankwo D.O., Moran L.S., Slatko B.E., Waite-Rees P.A., Dorner L.F., Benner J.S., Wilson G.G.
      Gene 150:75-80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiMTH3_HAEIN
    AccessioniPrimary (citable) accession number: P43871
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3