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Protein

Type-2 restriction enzyme HindIII

Gene

hindIIIR

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence AAGCTT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1151. HindIII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HindIII (EC:3.1.21.4)
Short name:
R.HindIII
Alternative name(s):
Endonuclease HindIII
Type II restriction enzyme HindIII
Gene namesi
Name:hindIIIR
Ordered Locus Names:HI_1393
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Type-2 restriction enzyme HindIIIPRO_0000077321Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1393.

Structurei

Secondary structure

1
300
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1511Combined sources
Turni16 – 183Combined sources
Helixi21 – 3313Combined sources
Helixi37 – 4711Combined sources
Helixi59 – 7820Combined sources
Beta strandi81 – 855Combined sources
Beta strandi94 – 10310Combined sources
Beta strandi105 – 1106Combined sources
Helixi122 – 1254Combined sources
Helixi127 – 1337Combined sources
Turni134 – 1363Combined sources
Beta strandi137 – 1448Combined sources
Helixi146 – 1483Combined sources
Beta strandi151 – 1533Combined sources
Helixi155 – 1639Combined sources
Beta strandi165 – 1695Combined sources
Helixi170 – 1789Combined sources
Helixi190 – 1934Combined sources
Helixi195 – 2006Combined sources
Helixi205 – 2073Combined sources
Helixi213 – 22412Combined sources
Helixi228 – 26134Combined sources
Helixi265 – 27511Combined sources
Helixi278 – 29316Combined sources
Helixi295 – 2984Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E52X-ray2.00A/B/C/D1-300[»]
3A4KX-ray2.17A/B/C/D1-300[»]
3WVGX-ray2.25A/B/C/D1-300[»]
3WVHX-ray2.54A/B/C/D1-300[»]
3WVIX-ray2.55A/B/C/D1-300[»]
3WVKX-ray2.00A/B/C/D1-300[»]
3WVPX-ray2.30A/B/C/D1-300[»]
ProteinModelPortaliP43870.
SMRiP43870. Positions 2-300.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43870.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG45031.
KOiK01155.
OMAiDINMSSK.
OrthoDBiEOG686NN8.

Family and domain databases

InterProiIPR019043. Restrct_endonuc_II_HindIII.
[Graphical view]
PfamiPF09518. RE_HindIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSALEKLL SLIENLTNQE FKQATNSLIS FIYKLNRNEV IELVRSIGIL
60 70 80 90 100
PEAIKPSSTQ EKLFSKAGDI VLAKAFQLLN LNSKPLEQRG NAGDVIALSK
110 120 130 140 150
EFNYGLVADA KSFRLSRTAK NQKDFKVKAL SEWREDKDYA VLTAPFFQYP
160 170 180 190 200
TTKSQIFKQS LDENVLLFSW EHLAILLQLD LEETNIFSFE QLWNFPKKQS
210 220 230 240 250
KKTSVSDAEN NFMRDFNKYF MDLFKIDKDT LNQLLQKEIN FIEERSLIEK
260 270 280 290 300
EYWKKQINII KNFTREEAIE ALLKDINMSS KIETIDSFIK GIKSNDRLYL
Length:300
Mass (Da):34,952
Last modified:November 1, 1995 - v1
Checksum:i62F62AC4B18E841B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15391 Genomic DNA. Translation: AAA61958.1.
L42023 Genomic DNA. Translation: AAC23040.1.
PIRiH64121.
RefSeqiNP_439547.1. NC_000907.1.
WP_010869201.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23040; AAC23040; HI_1393.
GeneIDi950303.
KEGGihin:HI1393.
PATRICi20191483. VBIHaeInf48452_1452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15391 Genomic DNA. Translation: AAA61958.1.
L42023 Genomic DNA. Translation: AAC23040.1.
PIRiH64121.
RefSeqiNP_439547.1. NC_000907.1.
WP_010869201.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E52X-ray2.00A/B/C/D1-300[»]
3A4KX-ray2.17A/B/C/D1-300[»]
3WVGX-ray2.25A/B/C/D1-300[»]
3WVHX-ray2.54A/B/C/D1-300[»]
3WVIX-ray2.55A/B/C/D1-300[»]
3WVKX-ray2.00A/B/C/D1-300[»]
3WVPX-ray2.30A/B/C/D1-300[»]
ProteinModelPortaliP43870.
SMRiP43870. Positions 2-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1393.

Chemistry

BindingDBiP43870.
ChEMBLiCHEMBL5004.

Protein family/group databases

REBASEi1151. HindIII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23040; AAC23040; HI_1393.
GeneIDi950303.
KEGGihin:HI1393.
PATRICi20191483. VBIHaeInf48452_1452.

Phylogenomic databases

eggNOGiNOG45031.
KOiK01155.
OMAiDINMSSK.
OrthoDBiEOG686NN8.

Miscellaneous databases

EvolutionaryTraceiP43870.
PROiP43870.

Family and domain databases

InterProiIPR019043. Restrct_endonuc_II_HindIII.
[Graphical view]
PfamiPF09518. RE_HindIII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, analysis and expression of the HindIII R-M-encoding genes."
    Nwankwo D.O., Moran L.S., Slatko B.E., Waite-Rees P.A., Dorner L.F., Benner J.S., Wilson G.G.
    Gene 150:75-80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiT2D3_HAEIN
AccessioniPrimary (citable) accession number: P43870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.