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P43870

- T2D3_HAEIN

UniProt

P43870 - T2D3_HAEIN

Protein

Type-2 restriction enzyme HindIII

Gene

hindIIIR

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (14 May 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence AAGCTT and cleaves after A-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1151. HindIII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HindIII (EC:3.1.21.4)
    Short name:
    R.HindIII
    Alternative name(s):
    Endonuclease HindIII
    Type II restriction enzyme HindIII
    Gene namesi
    Name:hindIIIR
    Ordered Locus Names:HI_1393
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Type-2 restriction enzyme HindIIIPRO_0000077321Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1393.

    Structurei

    Secondary structure

    1
    300
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1511
    Turni16 – 183
    Helixi21 – 3313
    Helixi37 – 4711
    Helixi59 – 7820
    Beta strandi81 – 855
    Beta strandi94 – 10310
    Beta strandi105 – 1106
    Helixi122 – 1254
    Helixi127 – 1337
    Turni134 – 1363
    Beta strandi137 – 1448
    Helixi146 – 1483
    Beta strandi151 – 1533
    Helixi155 – 1639
    Beta strandi165 – 1695
    Helixi170 – 1789
    Helixi190 – 1934
    Helixi195 – 2006
    Helixi205 – 2073
    Helixi213 – 22412
    Helixi228 – 26134
    Helixi265 – 27511
    Helixi278 – 29316
    Helixi295 – 2984

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E52X-ray2.00A/B/C/D1-300[»]
    3A4KX-ray2.17A/B/C/D1-300[»]
    ProteinModelPortaliP43870.
    SMRiP43870. Positions 2-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP43870.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG45031.
    KOiK01155.
    OMAiDINMSSK.
    OrthoDBiEOG686NN8.

    Family and domain databases

    InterProiIPR019043. Restrct_endonuc_II_HindIII.
    [Graphical view]
    PfamiPF09518. RE_HindIII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKSALEKLL SLIENLTNQE FKQATNSLIS FIYKLNRNEV IELVRSIGIL    50
    PEAIKPSSTQ EKLFSKAGDI VLAKAFQLLN LNSKPLEQRG NAGDVIALSK 100
    EFNYGLVADA KSFRLSRTAK NQKDFKVKAL SEWREDKDYA VLTAPFFQYP 150
    TTKSQIFKQS LDENVLLFSW EHLAILLQLD LEETNIFSFE QLWNFPKKQS 200
    KKTSVSDAEN NFMRDFNKYF MDLFKIDKDT LNQLLQKEIN FIEERSLIEK 250
    EYWKKQINII KNFTREEAIE ALLKDINMSS KIETIDSFIK GIKSNDRLYL 300
    Length:300
    Mass (Da):34,952
    Last modified:November 1, 1995 - v1
    Checksum:i62F62AC4B18E841B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15391 Genomic DNA. Translation: AAA61958.1.
    L42023 Genomic DNA. Translation: AAC23040.1.
    PIRiH64121.
    RefSeqiNP_439547.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC23040; AAC23040; HI_1393.
    GeneIDi950303.
    KEGGihin:HI1393.
    PATRICi20191483. VBIHaeInf48452_1452.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L15391 Genomic DNA. Translation: AAA61958.1 .
    L42023 Genomic DNA. Translation: AAC23040.1 .
    PIRi H64121.
    RefSeqi NP_439547.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E52 X-ray 2.00 A/B/C/D 1-300 [» ]
    3A4K X-ray 2.17 A/B/C/D 1-300 [» ]
    ProteinModelPortali P43870.
    SMRi P43870. Positions 2-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1393.

    Chemistry

    BindingDBi P43870.
    ChEMBLi CHEMBL5004.

    Protein family/group databases

    REBASEi 1151. HindIII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC23040 ; AAC23040 ; HI_1393 .
    GeneIDi 950303.
    KEGGi hin:HI1393.
    PATRICi 20191483. VBIHaeInf48452_1452.

    Phylogenomic databases

    eggNOGi NOG45031.
    KOi K01155.
    OMAi DINMSSK.
    OrthoDBi EOG686NN8.

    Miscellaneous databases

    EvolutionaryTracei P43870.

    Family and domain databases

    InterProi IPR019043. Restrct_endonuc_II_HindIII.
    [Graphical view ]
    Pfami PF09518. RE_HindIII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, analysis and expression of the HindIII R-M-encoding genes."
      Nwankwo D.O., Moran L.S., Slatko B.E., Waite-Rees P.A., Dorner L.F., Benner J.S., Wilson G.G.
      Gene 150:75-80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-26.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiT2D3_HAEIN
    AccessioniPrimary (citable) accession number: P43870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: May 14, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3