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Protein

Type-2 restriction enzyme HindIII

Gene

hindIIIR

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence AAGCTT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1151. HindIII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HindIII (EC:3.1.21.4)
Short name:
R.HindIII
Alternative name(s):
Endonuclease HindIII
Type II restriction enzyme HindIII
Gene namesi
Name:hindIIIR
Ordered Locus Names:HI_1393
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5004.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773211 – 300Type-2 restriction enzyme HindIIIAdd BLAST300

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1393.

Chemistry databases

BindingDBiP43870.

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 15Combined sources11
Turni16 – 18Combined sources3
Helixi21 – 33Combined sources13
Helixi37 – 47Combined sources11
Helixi59 – 78Combined sources20
Beta strandi81 – 85Combined sources5
Beta strandi89 – 91Combined sources3
Beta strandi94 – 103Combined sources10
Beta strandi105 – 110Combined sources6
Helixi122 – 125Combined sources4
Helixi127 – 133Combined sources7
Turni134 – 136Combined sources3
Beta strandi137 – 144Combined sources8
Helixi146 – 148Combined sources3
Beta strandi151 – 153Combined sources3
Helixi155 – 163Combined sources9
Beta strandi165 – 169Combined sources5
Helixi170 – 178Combined sources9
Helixi190 – 193Combined sources4
Helixi195 – 200Combined sources6
Helixi205 – 207Combined sources3
Helixi213 – 224Combined sources12
Helixi228 – 261Combined sources34
Helixi265 – 275Combined sources11
Helixi278 – 293Combined sources16
Helixi295 – 298Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E52X-ray2.00A/B/C/D1-300[»]
3A4KX-ray2.17A/B/C/D1-300[»]
3WVGX-ray2.25A/B/C/D1-300[»]
3WVHX-ray2.54A/B/C/D1-300[»]
3WVIX-ray2.55A/B/C/D1-300[»]
3WVKX-ray2.00A/B/C/D1-300[»]
3WVPX-ray2.30A/B/C/D1-300[»]
ProteinModelPortaliP43870.
SMRiP43870.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP43870.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105S7Q. Bacteria.
ENOG4111XCG. LUCA.
KOiK01155.
OMAiDINMSSK.

Family and domain databases

InterProiIPR019043. Restrct_endonuc_II_HindIII.
[Graphical view]
PfamiPF09518. RE_HindIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSALEKLL SLIENLTNQE FKQATNSLIS FIYKLNRNEV IELVRSIGIL
60 70 80 90 100
PEAIKPSSTQ EKLFSKAGDI VLAKAFQLLN LNSKPLEQRG NAGDVIALSK
110 120 130 140 150
EFNYGLVADA KSFRLSRTAK NQKDFKVKAL SEWREDKDYA VLTAPFFQYP
160 170 180 190 200
TTKSQIFKQS LDENVLLFSW EHLAILLQLD LEETNIFSFE QLWNFPKKQS
210 220 230 240 250
KKTSVSDAEN NFMRDFNKYF MDLFKIDKDT LNQLLQKEIN FIEERSLIEK
260 270 280 290 300
EYWKKQINII KNFTREEAIE ALLKDINMSS KIETIDSFIK GIKSNDRLYL
Length:300
Mass (Da):34,952
Last modified:November 1, 1995 - v1
Checksum:i62F62AC4B18E841B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15391 Genomic DNA. Translation: AAA61958.1.
L42023 Genomic DNA. Translation: AAC23040.1.
PIRiH64121.
RefSeqiNP_439547.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23040; AAC23040; HI_1393.
GeneIDi950303.
KEGGihin:HI1393.
PATRICi20191483. VBIHaeInf48452_1452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15391 Genomic DNA. Translation: AAA61958.1.
L42023 Genomic DNA. Translation: AAC23040.1.
PIRiH64121.
RefSeqiNP_439547.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E52X-ray2.00A/B/C/D1-300[»]
3A4KX-ray2.17A/B/C/D1-300[»]
3WVGX-ray2.25A/B/C/D1-300[»]
3WVHX-ray2.54A/B/C/D1-300[»]
3WVIX-ray2.55A/B/C/D1-300[»]
3WVKX-ray2.00A/B/C/D1-300[»]
3WVPX-ray2.30A/B/C/D1-300[»]
ProteinModelPortaliP43870.
SMRiP43870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1393.

Chemistry databases

BindingDBiP43870.
ChEMBLiCHEMBL5004.

Protein family/group databases

REBASEi1151. HindIII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23040; AAC23040; HI_1393.
GeneIDi950303.
KEGGihin:HI1393.
PATRICi20191483. VBIHaeInf48452_1452.

Phylogenomic databases

eggNOGiENOG4105S7Q. Bacteria.
ENOG4111XCG. LUCA.
KOiK01155.
OMAiDINMSSK.

Miscellaneous databases

EvolutionaryTraceiP43870.
PROiP43870.

Family and domain databases

InterProiIPR019043. Restrct_endonuc_II_HindIII.
[Graphical view]
PfamiPF09518. RE_HindIII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT2D3_HAEIN
AccessioniPrimary (citable) accession number: P43870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.