Reviewed, UniProtKB/Swiss-Prot P43864 (LON_HAEIN)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Synonyms:	lon-A
Ordered Locus Names:	HI0462

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	803 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 803	803	ATP-dependent protease La		PRO_0000076136
Regions
	Domain	8 – 200	193	Lon
	Nucleotide binding	356 – 363	8	ATP Potential
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43864-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9E590852611EEA5B	FASTA	803	89,347
	10 20 30 40 50 60 MAKNTQRTMP VLPLRDVVVF PYMVMPLFVG RAKSINALEE AMNDDKQILL VSQREADLEE 70 80 90 100 110 120 PTPEDLFDVG TIANIIQLLK LPDDTVKVLV EGQNRAKINS LEDGEKCFSA QITPIETTYG 130 140 150 160 170 180 DEKELVVAKS AVLSEFENYL TLNKKVPTDI LNALQRIDDV DRLADTMAAH LPVSIRHKQN 190 200 210 220 230 240 ALELANVQER LEYLLGMMES EADILQVEKR IRGRVKKQME KSQRNYYLNE QIKAIRKEMD 250 260 270 280 290 300 GGENEDTIDE VEQLHQKVEA AGMPADVRDK VENELQKLKM MSAMSSEATV IRSYIEWMIQ 310 320 330 340 350 360 VPWHQRSKVK KDIVKAQQVL DTDHYGLDRV KERILEYLAV QARLNKVKGP ILCLVGPPGV 370 380 390 400 410 420 GKTSLGQSIA NATGRKYVRM ALGGVRDEAE IRGHRKTYIG ALPGKLIQKM AKVGVKNPLF 430 440 450 460 470 480 LLDEIDKMAS DMRGDPASAL LEVLDPEQNT TFNDHYLEVD YDLSDVMFVA TSNSMNIPGP 490 500 510 520 530 540 LLDRMEVIRL SGYTEDEKLN IAMRHLLAKQ IERNGLKKGE LTVEESAILD IIRYYTREAG 550 560 570 580 590 600 VRGLEREISK ICRKAVKNLL VNPKLKSITV NSDNLHDYLG VKRFEFGKAD TQNRIGEVTG 610 620 630 640 650 660 LAWTEVGGDL LTIETASVVG KGKLSFTGSL GDVMKESIQA AMTVVRARAD KLGINAEFHE 670 680 690 700 710 720 KRDIHIHVPD GATPKDGPSA GIAMCTALIS CLTGNPVRAD VAMTGEISLR GKVLPIGGLK 730 740 750 760 770 780 EKLLAAHRGG IKTVLIPKEN VKDLEEIPEN VKQNLAIHAV ETIDEVLGFA LENPPEGIEF 790 800 VKVEAKPKAP RRKVTSKSER AVN

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22121.1.
PIR	A64070.
RefSeq	NP_438623.1.
3D structure databases
SMR	P43864. Positions 491-584, 594-781.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Genome annotation databases
GeneID	949549.
GenomeReviews	Gene locus HI0462 in contig L42023_GR.
KEGG	hin:HI0462.
NMPDR	fig|71421.1.peg.436.
TIGR	HI0462.
Phylogenomic databases
HOGENOM	P43864.
OMA	P43864. VMKESIQ.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0462-MON.
BRENDA	3.4.21.53. 109.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_HAEIN

Accession

Primary (citable) accession number: P43864

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)