Reviewed, UniProtKB/Swiss-Prot P43850 (PURK_HAEIN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	HI1616

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000074998
Regions
	Domain	89 – 274	186	ATP-grasp
	Nucleotide binding	158 – 161	4	ATP By similarity
	Nucleotide binding	244 – 245	2	ATP By similarity
Sites
	Binding site	85	1	ATP By similarity
	Binding site	125	1	ATP By similarity
	Binding site	136	1	ATP By similarity
	Binding site	166	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43850-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 90CE70504784B452	FASTA	362	41,273
	10 20 30 40 50 60 MQNSTLYPTV YVLGNGQLGR MLRYAGAPLD IYVEPLAFNA PVFDLPENAI ITAEIERWEK 70 80 90 100 110 120 TPLTELLGNH KNFVNQHIFG LLADRFTQKS LLDELNLSTS PWCLLKDKNQ WNDLFQTVGE 130 140 150 160 170 180 KVVVKRRTGG YDGRGQWIIR DENRADITDD LFGEVIAEKF IPFDYEVSIV GARFKNGEKR 190 200 210 220 230 240 FYPVTHNLQQ NGILRYSVVD CAFPQQSVQQ KQAETMLGKI MDKLGYVGVM AMECFVVGDK 250 260 270 280 290 300 LLINELAPRV HNSGHWTQLG CSISQFELHL RALLNLPTPE LQTFAPSVMI NLIGTNHNPK 310 320 330 340 350 360 WLNIPFAQLH WYGKEVRIGR KVGHINLSHP NKAVIIQQLE KLCTELPEDY QSGLNWAIEK LK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC23264.1.
PIR	H64132.
RefSeq	NP_439758.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Genome annotation databases
GeneID	950468.
GenomeReviews	Gene locus HI1616 in contig L42023_GR.
KEGG	hin:HI1616.
NMPDR	fig|71421.1.peg.1532.
TIGR	HI1616.
Phylogenomic databases
HOGENOM	P43850.
OMA	TFAPSVM.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1616-MON.
BRENDA	4.1.1.21. 109.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_HAEIN

Accession

Primary (citable) accession number: P43850

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)