P43849 (PURE_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutase
Short name=N5-CAIR mutase
EC=5.4.99.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide mutase

Gene names

Name:	purE
Ordered Locus Names:	HI_1615

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) By similarity.
Catalytic activity	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	N5-carboxyaminoimidazole ribonucleotide mutase		PRO_0000074975
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	43	1	Substrate By similarity
Experimental info
	Sequence conflict	139 – 143	5	DALFT → AELLS in CAA83909. Ref.2
	Sequence conflict	147	1	A → S in CAA83909. Ref.2
	Sequence conflict	151	1	N → S in CAA83909. Ref.2
	Sequence conflict	154 – 155	2	NM → RA in CAA83909. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43849 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 60A03B0D4DD85412	FASTA	164	17,269
	10 20 30 40 50 60 MSKTAQIAVV MGSKSDWATM QEATQILDEL NVPYHVEVVS AHRTPDKLFE FAENAQKNGY 70 80 90 100 110 120 KVIIAGAGGA AHLPGMIAAK TLVPVLGVPV KSSMLSGVDS LYSIVQMPKG IPVGTLAIGP 130 140 150 160 AGAANAGLLA AQILAGWDDA LFTRLQAFRE NQTNMVLDNP DPRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"The fimbrial gene cluster of Haemophilus influenzae type b." van Ham M.S., van Alphen L., Mooi F.R., van Putten J.P.M. Mol. Microbiol. 13:673-684(1994) [PubMed: 7997179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-161. Strain: AM30 (770235) / Serotype B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC23263.1. Z33502 Genomic DNA. Translation: CAA83909.1.
PIR	G64132. S54434.
RefSeq	NP_439757.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43849.
SMR	P43849. Positions 5-163.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949616.
GenomeReviews	Gene locus HI_1615 in contig L42023_GR.
KEGG	hin:HI1615.
NMPDR	fig|71421.1.peg.1531.
PATRIC	20191961. VBIHaeInf48452_1688.
TIGR	HI_1615.
Phylogenomic databases
HOGENOM	HBG301965.
OMA	LIMGSDS.
PhylomeDB	P43849.
ProtClustDB	CLSK870172.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1615-MONOMER.
Family and domain databases
InterPro	IPR024694. N5-CAIR_mutase_PurE. IPR000031. N5-CAIR_Mutase_PurE_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
KO	K01588.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
PIRSF	PIRSF001338. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURE_HAEIN

Accession

Primary (citable) accession number: P43849

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program