Reviewed, UniProtKB/Swiss-Prot P43846 (PUR3_HAEIN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
      Short name=GART

Gene names

Name:	purN
Ordered Locus Names:	HI1428

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Phosphoribosylglycinamide formyltransferase		PRO_0000074944
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	90 – 93	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	109	1	Proton donor By similarity
	Binding site	107	1	10-formyltetrahydrofolate By similarity
	Binding site	145	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	174	1	5'-phosphoribosylglycinamide By similarity
	Site	145	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43846-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D1AE249CDDB879C4	FASTA	212	23,463
	10 20 30 40 50 60 MKKIAVLISG QGTNLQTIID ACHSGDIPAK IACVISNKAD AYGLVRAKQA QIPQAVFLRK 70 80 90 100 110 120 NFSNNLEMDD AIGDYLQSLA VDLIVLAGYM KILTPKFTQR FAGKILNIHP SLLPKYAGLN 130 140 150 160 170 180 TYQRAIEAGD NEHGTTVHFV NEEVDGGAIV LQAKVPIFPE DSIEEVEART REQEYQIYPL 190 200 210 VIKWFTEGRL RLKDNLAYLD GKALPKSGYA NE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC23075.1.
PIR	F64122.
RefSeq	NP_439577.1.
3D structure databases
HSSP	HSSP built from PDB template 1GAR based on UniProtKB P08179.
ModBase	Search...
Genome annotation databases
GeneID	950331.
GenomeReviews	Gene locus HI1428 in contig L42023_GR.
KEGG	hin:HI1428.
NMPDR	fig|71421.1.peg.1360.
TIGR	HI1428.
Phylogenomic databases
HOGENOM	P43846.
OMA	CSVHFVT.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1428-MON.
BRENDA	2.1.2.2. 109.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_HAEIN

Accession

Primary (citable) accession number: P43846

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)