Reviewed, UniProtKB/Swiss-Prot P43843 (ASPG2_HAEIN)

Names and origin

Protein names

Recommended name:
    Probable L-asparaginase periplasmic
      Short name=L-ASNase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase

Gene names

Name:	ansB
Ordered Locus Names:	HI0745

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	asparagine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 349	328	Probable L-asparaginase periplasmic		PRO_0000002357
Sites
	Active site	35	1	By similarity
	Active site	112	1	By similarity
	Active site	113	1	By similarity
	Active site	185	1	By similarity
Amino acid modifications
	Disulfide bond	100 ↔ 128		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43843-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 45EA79CAC215F8A9	FASTA	349	37,212
	10 20 30 40 50 60 MKLTKLALCT LFGLGVSIAN AADLPNITIL ATGGTIAGSG QSSVNSAYKA GQLSIDTLIE 70 80 90 100 110 120 AVPEMKNIAN IKGEQIVKIG SQDMNDEVWL KLAKAINAQC KSTDGFVITH GTDTMEETAY 130 140 150 160 170 180 FLDLTVKCEK PVVLVGAMRP ATEKSADGPL NLYNAVVVAA DKKSSGRGVL VAMNNEVLGA 190 200 210 220 230 240 RDVTKTSTTA VQTFHSPNYG SLGYIHNSKV DYERSPESKH TINTPFNVEK LDSLPKVGII 250 260 270 280 290 300 YAYSNAPVEP LNALLNAGYQ GIVSAGVGNG NVNAAHLDRL EKAAKDSVVV VRSSRVPTGY 310 320 330 340 TTRDAEVDDS KYGFVASGTL NPQKARVLLQ LALTQTKDPK VIQQYFEDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed: 10675023] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-26.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22403.1.
PIR	A64090.
RefSeq	NP_438904.1.
3D structure databases
SMR	P43843. Positions 24-349.
ModBase	Search...
Genome annotation databases
GeneID	949675.
GenomeReviews	Gene locus HI0745 in contig L42023_GR.
KEGG	hin:HI0745.
NMPDR	fig|71421.1.peg.713.
TIGR	HI0745.
Phylogenomic databases
HOGENOM	HBG497495.
OMA	TLEESAY.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0745-MONOMER.
BRENDA	3.5.1.1. 109.
Family and domain databases
InterPro	IPR004550. AsnASE_II. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00520. asnASE_II. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG2_HAEIN

Accession

Primary (citable) accession number: P43843

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)