Reviewed, UniProtKB/Swiss-Prot P43842 (PNTA_HAEIN)

Names and origin

Protein names

Recommended name:
    NAD(P) transhydrogenase subunit alpha
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase subunit alpha
    Pyridine nucleotide transhydrogenase subunit alpha

Gene names

Name:	pntA
Ordered Locus Names:	HI1362

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane By similarity.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	NAD(P) transhydrogenase subunit alpha		PRO_0000199018
Regions
	Transmembrane	401 – 421	21	Potential
	Transmembrane	422 – 442	21	Potential
	Transmembrane	452 – 472	21	Potential
	Transmembrane	479 – 499	21	Potential
	Nucleotide binding	167 – 197	31	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43842-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 50D63AD536E837EF	FASTA	512	54,973
	10 20 30 40 50 60 MLIGVPRELL ENESRVAATP KTVQQILKLG FDVIVEHDAG FKASFEDQAF LEAGAKIGTS 70 80 90 100 110 120 AEIWQSDIIF KVNAPTDEEI AQMKEGAALV SFIWRMQNPE LMKKLTAKKI NVLAMDAVPR 130 140 150 160 170 180 ISRAQALDAL SSMANISGYR AVIEAAHEFG SFFTGQITAA GKVPPAKVLV IGAGVAGLAA 190 200 210 220 230 240 IGAANSLGAI VRAFDSRPEV KEQVQSMGAS FLEIDFKEEG GSGDGYAKVM SEEFNRRAME 250 260 270 280 290 300 LYAEQAKEVD IIITTAAIPG KPAPRLITKE MVDSMKPGSV IVDLAAATGG NCEYTQAGKV 310 320 330 340 350 360 VTTENQVKVI GYTDFPSRLP TQSSQLYGTN LVNLLKLLCK EKDGNINIDF EDVVLRGVTV 370 380 390 400 410 420 VRDGEEIPPA QIQVSAQPKQ ETKAAPVAEK KESKPTDPRV KYGVMAGVGV LFLWLASVAP 430 440 450 460 470 480 AAFLSHFTVF VLACVVGYYV VWNVSHALHT PLMAVTNAIS GIIIVGALLQ IRQPTGNLFI 490 500 510 DALAFVAILV ASINIFGGFR VTQRMLAMFR KG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC23009.1.
PIR	E64119.
RefSeq	NP_439513.1.
3D structure databases
HSSP	HSSP built from PDB template 1L7E based on UniProtKB Q60164.
SMR	P43842. Positions 3-375.
ModBase	Search...
Genome annotation databases
GeneID	950278.
GenomeReviews	Gene locus HI1362 in contig L42023_GR.
KEGG	hin:HI1362.
NMPDR	fig|71421.1.peg.1298.
TIGR	HI1362.
Phylogenomic databases
HOGENOM	P43842.
OMA	FKVNAPT.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1362-MON.
BRENDA	1.6.1.2. 109.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR016040. NAD(P)-bd_dom. IPR004571. NADP_transhyd_a. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00561. pntA. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNTA_HAEIN

Accession

Primary (citable) accession number: P43842

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)