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Protein

NAD(P) transhydrogenase subunit alpha

Gene

pntA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.By similarity

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175NAD; via amide nitrogenBy similarity1
Binding sitei225NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 128NADBy similarity4
Nucleotide bindingi195 – 197NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase subunit alpha (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit alpha
Pyridine nucleotide transhydrogenase subunit alpha
Gene namesi
Name:pntA
Ordered Locus Names:HI_1362
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 400CytoplasmicSequence analysisAdd BLAST400
Transmembranei401 – 421HelicalSequence analysisAdd BLAST21
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 451CytoplasmicSequence analysis9
Transmembranei452 – 472HelicalSequence analysisAdd BLAST21
Topological domaini473 – 478PeriplasmicSequence analysis6
Transmembranei479 – 499HelicalSequence analysisAdd BLAST21
Topological domaini500 – 512CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001990181 – 512NAD(P) transhydrogenase subunit alphaAdd BLAST512

Proteomic databases

PRIDEiP43842.

Interactioni

Subunit structurei

Heterodimer of an alpha (PntA) and a beta (PntB) chain.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi71421.HI1362.

Structurei

3D structure databases

ProteinModelPortaliP43842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108IIE. Bacteria.
COG3288. LUCA.
KOiK00324.
OMAiNVAPKEF.
PhylomeDBiP43842.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR008143. Ala_DH/PNT_CS2.
IPR008142. AlaDH/PNT_CS1.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR023753. FAD/NAD-binding_dom.
IPR016040. NAD(P)-bd_dom.
IPR026255. NADP_transhyd_a.
IPR024605. NADP_transhyd_a_C.
[Graphical view]
PfamiPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF12769. PNTB_4TM. 1 hit.
[Graphical view]
PIRSFiPIRSF000203. NADP_transhydrogenase_alpha. 1 hit.
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00561. pntA. 1 hit.
PROSITEiPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGVPRELL ENESRVAATP KTVQQILKLG FDVIVEHDAG FKASFEDQAF
60 70 80 90 100
LEAGAKIGTS AEIWQSDIIF KVNAPTDEEI AQMKEGAALV SFIWRMQNPE
110 120 130 140 150
LMKKLTAKKI NVLAMDAVPR ISRAQALDAL SSMANISGYR AVIEAAHEFG
160 170 180 190 200
SFFTGQITAA GKVPPAKVLV IGAGVAGLAA IGAANSLGAI VRAFDSRPEV
210 220 230 240 250
KEQVQSMGAS FLEIDFKEEG GSGDGYAKVM SEEFNRRAME LYAEQAKEVD
260 270 280 290 300
IIITTAAIPG KPAPRLITKE MVDSMKPGSV IVDLAAATGG NCEYTQAGKV
310 320 330 340 350
VTTENQVKVI GYTDFPSRLP TQSSQLYGTN LVNLLKLLCK EKDGNINIDF
360 370 380 390 400
EDVVLRGVTV VRDGEEIPPA QIQVSAQPKQ ETKAAPVAEK KESKPTDPRV
410 420 430 440 450
KYGVMAGVGV LFLWLASVAP AAFLSHFTVF VLACVVGYYV VWNVSHALHT
460 470 480 490 500
PLMAVTNAIS GIIIVGALLQ IRQPTGNLFI DALAFVAILV ASINIFGGFR
510
VTQRMLAMFR KG
Length:512
Mass (Da):54,973
Last modified:November 1, 1995 - v1
Checksum:i50D63AD536E837EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23009.1.
PIRiE64119.
RefSeqiNP_439513.1. NC_000907.1.
WP_005693998.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23009; AAC23009; HI_1362.
GeneIDi950278.
KEGGihin:HI1362.
PATRICi20191411. VBIHaeInf48452_1416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23009.1.
PIRiE64119.
RefSeqiNP_439513.1. NC_000907.1.
WP_005693998.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1362.

Proteomic databases

PRIDEiP43842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23009; AAC23009; HI_1362.
GeneIDi950278.
KEGGihin:HI1362.
PATRICi20191411. VBIHaeInf48452_1416.

Phylogenomic databases

eggNOGiENOG4108IIE. Bacteria.
COG3288. LUCA.
KOiK00324.
OMAiNVAPKEF.
PhylomeDBiP43842.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR008143. Ala_DH/PNT_CS2.
IPR008142. AlaDH/PNT_CS1.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR023753. FAD/NAD-binding_dom.
IPR016040. NAD(P)-bd_dom.
IPR026255. NADP_transhyd_a.
IPR024605. NADP_transhyd_a_C.
[Graphical view]
PfamiPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF12769. PNTB_4TM. 1 hit.
[Graphical view]
PIRSFiPIRSF000203. NADP_transhydrogenase_alpha. 1 hit.
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00561. pntA. 1 hit.
PROSITEiPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNTA_HAEIN
AccessioniPrimary (citable) accession number: P43842
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.