Reviewed, UniProtKB/Swiss-Prot P43837 (MAO2_HAEIN)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:	maeB
Ordered Locus Names:	HI1245

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	In the N-terminal section; belongs to the malic enzymes family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro acyltransferase activity Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	NADP-dependent malic enzyme		PRO_0000160243
Regions
	Nucleotide binding	76 – 83	8	NADP By similarity
	Region	1 – 428	428	Malic enzyme
	Region	429 – 756	328	Phosphate acetyltransferase
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	136	1	Divalent metal cation By similarity
	Metal binding	137	1	Divalent metal cation By similarity
	Binding site	162	1	NAD By similarity
	Binding site	288	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43837-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8D303736F08B8E3B	FASTA	756	81,880
	10 20 30 40 50 60 MTEQLRQAAL DFHEFPIPGK IEVTPTKSLA TQRDLALAYS PGVAEPCLEI EKDPAASYKY 70 80 90 100 110 120 TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGINVFDIE VNEHDPDKLV 130 140 150 160 170 180 DIIASLEPTF GGVNLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS AAAIINSLRI 190 200 210 220 230 240 VGKKIEDVRL VASGAGAASI ACLNLLLSLG MKRENITVCD SKGVVYKGRD DKMDQTKKEY 250 260 270 280 290 300 AIEDNGWRKL ADAIPNADIF LGCSAAGALT QDMVKSMAAH PIILALANPN PEITPPEAKA 310 320 330 340 350 360 VRPDAIVCTG RSDYPNQVNN VLCFPFIFRG ALDVGATTIN EEMKRAAVYA IADLALEEQN 370 380 390 400 410 420 EVVTSAYGGE GATFGADYVI PRPFDPRLIV RIAPAVAKAA MESGVATRPI QNWDAYVEKL 430 440 450 460 470 480 TQFVYKTSLF MRPIFSQAKS AKQRIILAEG EENKALHATQ EVISMGLANP ILIGRRSVIE 490 500 510 520 530 540 EKIKKLGLRL TAGVDFEIVD NEDNPRYEEC WKHYYELTKR KGITPAIAKR VVRSNTTVLA 550 560 570 580 590 600 STLLSLGYAD ALVCGLFGSY GKHLASIRDI IGLKDGVKTA AALNSLVLPT GNVFLTDTHV 610 620 630 640 650 660 NSNPTAEELA EITLMAAEEI HRFGIEPAVA LLSHSNFGSS DSLGAPKMRE VLQIVKERNP 670 680 690 700 710 720 HLMIDGEMRG DLAMNEAHRK ELMPDSPLKG SANLLVFPDL SASRISYSLL RGTTTAITVG 730 740 750 PILMGMNKSA HILNPGASVR RIINMIAYAA VKAQQE

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22896.1.
PIR	F64112.
RefSeq	NP_439401.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	949562.
GenomeReviews	Gene locus HI1245 in contig L42023_GR.
KEGG	hin:HI1245.
NMPDR	fig|71421.1.peg.1191.
TIGR	HI1245.
Phylogenomic databases
HOGENOM	P43837.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1245-MON.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR012188. ME_PTA. IPR016040. NAD(P)-bd. IPR002505. PTA_PTB. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF036684. ME_PTA. 1 hit.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO2_HAEIN

Accession

Primary (citable) accession number: P43837

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)