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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:HI_0921
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Leucine--tRNA ligasePRO_0000152023Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0921.

Structurei

3D structure databases

ProteinModelPortaliP43827.
SMRiP43827. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiP43827.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43827-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQEQYRPDMI EPKVQQYWAE NKVFKAIKDE SKEKYYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMLG KNVLQPFGWD AFGLPAEGAA IKNKTAPAKW
110 120 130 140 150
TYENIAYMKK QLQLLGFGFD WDREIATCKP EYYKWEQWFF TELYKKGLVY
160 170 180 190 200
KKTSTVNWCP NDETVLANEQ VHEGCCWRCD TPVEQKEIPQ WFIKITDYAE
210 220 230 240 250
QLLGGLDTLP QWPDMVKTMQ RNWIGRSEGV EITFDVANTN EKVAVYTTRP
260 270 280 290 300
DTFYGVSYLG IAAAHPLASL AAQNNSELAA FIQEAKNAKV AEADLATMEK
310 320 330 340 350
KGMATGLFAI HPLTGDKLPI WVANFVLMHY GTGAVMAVPA HDQRDFEFAQ
360 370 380 390 400
KYSLPIKQVI APLADEEIDL TKQAFVEHGK LVNSDEFDGK NFDGAFNGIA
410 420 430 440 450
DKLEKLGVGK RQVNYRLRDW GVSRQRYWGA PIPMLTLENG DVVPAPMEDL
460 470 480 490 500
PIILPEDVVM DGVKSPINAD PNWAKTTFND APALKETDTF DTFMESSWYY
510 520 530 540 550
ARYTCPQYQN GMLDAEEANY WLPVDQYIGG IEHATMHLLY FRFFHKLLRD
560 570 580 590 600
AGFVTSEEPA DKLLCQGMVL ADAFYYTSPT NERIWVSPTQ VTLERDEKGR
610 620 630 640 650
IIKATDPEGR ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS
660 670 680 690 700
PAEMTLEWQE SGVEGAKRFL GRVWNLVYQY QQNPAKTSLD LTALSAEQKV
710 720 730 740 750
LRREVHKTIA KVSDDIGRRQ TFNTAIAAVM ELMNKLTKAS LDSEQDRAVM
760 770 780 790 800
AEALSAVVRM LYPITPHICF ELWQALGNES AIDTAEWVKA DEAAMVEDEK
810 820 830 840 850
LIVVQVNGKV RGKVTVATDA DEDTVKTIAF ADENVKKFID NQHIVKVIYV
860
VGKLLNVVVK P
Length:861
Mass (Da):97,751
Last modified:November 1, 1995 - v1
Checksum:iEB93304F6B4C8FB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22581.1.
PIRiH64102.
RefSeqiNP_439081.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22581; AAC22581; HI_0921.
GeneIDi949390.
KEGGihin:HI0921.
PATRICi20190497. VBIHaeInf48452_0962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22581.1.
PIRiH64102.
RefSeqiNP_439081.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP43827.
SMRiP43827. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22581; AAC22581; HI_0921.
GeneIDi949390.
KEGGihin:HI0921.
PATRICi20190497. VBIHaeInf48452_0962.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiP43827.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiSYL_HAEIN
AccessioniPrimary (citable) accession number: P43827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.