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P43824

- SYI_HAEIN

UniProt

P43824 - SYI_HAEIN

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Protein
Isoleucine--tRNA ligase
Gene
ileS, HI_0962
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylate By similarity
Binding sitei606 – 6061ATP By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity
Metal bindingi927 – 9271Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HI_0962
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098395Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP43824.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi603 – 6075"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43824-1 [UniParc]FASTAAdd to Basket

« Hide

MTVDYKNTLN LPETSFPMRG DLAKREPDKL KNWYEKNLYQ KIRKASKGKK    50
SFILHDGPPY ANGNIHIGHA VNKILKDIII KSKTALGFDS PYIPGWDCHG 100
LPIELKVEGL VGKPNEKISA AEFRQKCREY AAEQVEGQKK DFIRLGVLGD 150
WDNPYLTMNF DTEANIIRTL GKVIENGHLY KGSKPVHWCL DCGSSLAEAE 200
VEYEDKVSPS IYVRFPAESA DEIEAKFSAQ GRGQGKLSAI IWTTTPWTMP 250
SNRAIAVNAD LEYNLVQLGD ERVILAAELV ESVAKAVGIE HIEILGSVKG 300
DDLELSRFHH PFYDFTVPVI LGDHVTTDGG TGLVHTAPDH GLDDFIVGKQ 350
YDLPMAGLVS NDGKFISTTE FFAGKGVFEA NPLVIEKLQE VGNLLKVEKI 400
KHSYPHCWRH KTPIIFRATP QWFIGMETQG LRQQALGEIK QVRWIPDWGQ 450
ARIEKMVENR PDWCISRQRT WGVPMTLFVH KETEELHPRT LDLLEEVAKR 500
VERAGIQAWW DLDEKELLGA DAETYRKVPD TLDVWFDSGS TYSSVVANRL 550
EFNGQDIDMY LEGSDQHRGW FMSSLMLSTA TDSKAPYKQV LTHGFTVDGQ 600
GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEMT VSDEILKRAA 650
DSYRRIRNTA RFLLANLNGF DPKRDLVKPE KMISLDRWAV ACALDAQNEI 700
KDAYDNYQFH TVVQRLMRFC SVEMGSFYLD IIKDRQYTTK ADSLARRSCQ 750
TALWHIAEAL VRWMAPILSF TADEIWQHLP QTESARAEFV FTEEFYQGLF 800
GLGEDEKLDD AYWQQLIKVR SEVNRVLEIS RNNKEIGGGL EAEVTVYAND 850
EYRALLAQLG NELRFVLITS KVDVKSLSEK PADLADSELE GIAVSVTRSN 900
AEKCPRCWHY SDEIGVSPEH PTLCARCVEN VVGNGEVRYF A 941
Length:941
Mass (Da):106,529
Last modified:January 9, 2007 - v2
Checksum:i35B9C66B921B1DAF
GO

Sequence cautioni

The sequence L42023 differs from that shown. Reason: Erroneous termination at position 30. Translated as Leu.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. No translation available.
PIRiS78633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. No translation available.
PIRi S78633.

3D structure databases

ProteinModelPortali P43824.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OMAi KPVHWCL.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiSYI_HAEIN
AccessioniPrimary (citable) accession number: P43824
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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