P43812 (PYRF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotidine 5'-phosphate decarboxylase
EC=4.1.1.23

Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase

Gene names

Name:	pyrF
Ordered Locus Names:	HI_1224

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP-Rule MF_01200
Catalytic activity	Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01200
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP-Rule MF_01200
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the OMP decarboxylase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway 'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Orotidine 5'-phosphate decarboxylase HAMAP-Rule MF_01200		PRO_0000134546
Regions
	Region	59 – 68	10	Substrate binding By similarity
Sites
	Active site	61	1	Proton donor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	209	1	Substrate; via amide nitrogen By similarity
	Binding site	210	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43812 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 14E8E825DBC767D9	FASTA	230	25,192
	10 20 30 40 50 60 MTSKIIVALD FEKEAEALAL VDQIDPSLCR LKVGKEMFTT LGINFVKQLH QRNFDVFLDL 70 80 90 100 110 120 KYHDIPNTVA RAVRSAADLG VWMVDLHASG GLRMMEDAKK ILEPYGKDAP LLIAVTVLTS 130 140 150 160 170 180 MEDLDLLQIG INASPMEQVL RLAHLTQRAG LDGVVCSPQE VEILRNACGE DFKLVTPGIR 190 200 210 220 230 PIGTDFGDQR RVMTPTAAIR AGSDYLVIGR PITQADNPAE VLRSINVSIG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22877.1.
PIR	B64111.
RefSeq	NP_439380.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43812.
SMR	P43812. Positions 3-229.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22877; AAC22877; HI_1224.
GeneID	950791.
KEGG	hin:HI1224.
PATRIC	20191127. VBIHaeInf48452_1276.
Phylogenomic databases
eggNOG	COG0284.
KO	K01591.
OMA	NFKIFLD.
ProtClustDB	PRK00230.
Enzyme and pathway databases
UniPathway	UPA00070; UER00120.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01200_B. OMPdecase_type1_B.
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR018089. OMPdecase_AS. IPR001754. OMPdeCOase_dom. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00215. OMPdecase. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRF_HAEIN

Accession

Primary (citable) accession number: P43812

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program