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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathway:ippGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:HI_1741
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166571Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1741.

Structurei

3D structure databases

ProteinModelPortaliP43811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 117100HDAdd
BLAST
Domaini601 – 67575ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
KOiK01139.
OMAiREMHEID.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARDAHEGQ FRSSGEPYIT HPVAVASIIA QLHLDHEAVM AALLHDVIED
60 70 80 90 100
TPYTEEQLKE EFGASVAEIV DGVSKLDKLK FRTRQEAQVE NFRKMILAMT
110 120 130 140 150
RDIRVVLIKL ADRTHNMRTL GSLRPDKRRR IAKETLEIYC PLAHRLGIEH
160 170 180 190 200
IKNELEDLSF QAMHPHRYEV LKKLVDVARS NRQDLIERIS QEIKVRLENS
210 220 230 240 250
GIFARVWGRE KHLYKIYQKM RIKDQEFHSI MDIYAFRVIV KNVDDCYRVL
260 270 280 290 300
GQMHNLYKPR PGRVKDYIAV PKANGYQSLQ TSMIGPKGVP VEVHIHTEDM
310 320 330 340 350
EQVAEMGITA HWVYKENGKN DSTTAQIRVQ RWLQSLVEIQ QSVGNSFEFI
360 370 380 390 400
ENVKSEFFPK EIYVFTPKGR IVELPMGATA VDFAYAVHSD VGNTCVGVTV
410 420 430 440 450
EHKPYPLSKA LESGQTVNII TDPNAHPEVA WLNFVVTARA KTRIRHYLKQ
460 470 480 490 500
RCEEDAVKLG EVELNVALQP HNLGDFSIQQ IRTVLDALAL SSLDELLREI
510 520 530 540 550
GLGNQSASMI AHQFVGVPLE SANTKNLEFE SKILTIAPMQ VGKTQFAQCC
560 570 580 590 600
HPILGDPIVG CCTEKNTVVV HHQHCASLKN ACRQSLAKWD NVQSAVNFEA
610 620 630 640 650
ELQIEILNEQ NALLSLMTAI SASESSLQNI WTEELENNLL LVILQVCVKD
660 670
IKHLANIVHR IKGITGVVNV KRNINEL
Length:677
Mass (Da):76,726
Last modified:November 1, 1995 - v1
Checksum:iFB12CE3C33A18B93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23388.1.
PIRiF64139.
RefSeqiNP_439885.2. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23388; AAC23388; HI_1741.
GeneIDi950884.
KEGGihin:HI1741.
PATRICi20192253. VBIHaeInf48452_1824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC23388.1.
PIRiF64139.
RefSeqiNP_439885.2. NC_000907.1.

3D structure databases

ProteinModelPortaliP43811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC23388; AAC23388; HI_1741.
GeneIDi950884.
KEGGihin:HI1741.
PATRICi20192253. VBIHaeInf48452_1824.

Phylogenomic databases

eggNOGiCOG0317.
KOiK01139.
OMAiREMHEID.
OrthoDBiEOG6SV551.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiSPOT_HAEIN
AccessioniPrimary (citable) accession number: P43811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.