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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandManganese

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:HI_1741
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001665711 – 677Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolaseAdd BLAST677

Proteomic databases

PRIDEiP43811

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1741

Structurei

3D structure databases

ProteinModelPortaliP43811
SMRiP43811
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 117HDPROSITE-ProRule annotationAdd BLAST100
Domaini601 – 675ACTPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated

Phylogenomic databases

eggNOGiENOG4105CWR Bacteria
COG0317 LUCA
KOiK01139
OMAiHYYSIYQ

Family and domain databases

CDDicd00077 HDc, 1 hit
cd05399 NT_Rel-Spo_like, 1 hit
cd01668 TGS_RelA_SpoT, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR012675 Beta-grasp_dom_sf
IPR003607 HD/PDEase_dom
IPR004811 RelA/Spo_fam
IPR007685 RelA_SpoT
IPR004095 TGS
IPR012676 TGS-like
IPR033655 TGS_RelA
PfamiView protein in Pfam
PF13291 ACT_4, 1 hit
PF13328 HD_4, 1 hit
PF04607 RelA_SpoT, 1 hit
PF02824 TGS, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
SM00954 RelA_SpoT, 1 hit
SUPFAMiSSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00691 spoT_relA, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

P43811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARDAHEGQ FRSSGEPYIT HPVAVASIIA QLHLDHEAVM AALLHDVIED
60 70 80 90 100
TPYTEEQLKE EFGASVAEIV DGVSKLDKLK FRTRQEAQVE NFRKMILAMT
110 120 130 140 150
RDIRVVLIKL ADRTHNMRTL GSLRPDKRRR IAKETLEIYC PLAHRLGIEH
160 170 180 190 200
IKNELEDLSF QAMHPHRYEV LKKLVDVARS NRQDLIERIS QEIKVRLENS
210 220 230 240 250
GIFARVWGRE KHLYKIYQKM RIKDQEFHSI MDIYAFRVIV KNVDDCYRVL
260 270 280 290 300
GQMHNLYKPR PGRVKDYIAV PKANGYQSLQ TSMIGPKGVP VEVHIHTEDM
310 320 330 340 350
EQVAEMGITA HWVYKENGKN DSTTAQIRVQ RWLQSLVEIQ QSVGNSFEFI
360 370 380 390 400
ENVKSEFFPK EIYVFTPKGR IVELPMGATA VDFAYAVHSD VGNTCVGVTV
410 420 430 440 450
EHKPYPLSKA LESGQTVNII TDPNAHPEVA WLNFVVTARA KTRIRHYLKQ
460 470 480 490 500
RCEEDAVKLG EVELNVALQP HNLGDFSIQQ IRTVLDALAL SSLDELLREI
510 520 530 540 550
GLGNQSASMI AHQFVGVPLE SANTKNLEFE SKILTIAPMQ VGKTQFAQCC
560 570 580 590 600
HPILGDPIVG CCTEKNTVVV HHQHCASLKN ACRQSLAKWD NVQSAVNFEA
610 620 630 640 650
ELQIEILNEQ NALLSLMTAI SASESSLQNI WTEELENNLL LVILQVCVKD
660 670
IKHLANIVHR IKGITGVVNV KRNINEL
Length:677
Mass (Da):76,726
Last modified:November 1, 1995 - v1
Checksum:iFB12CE3C33A18B93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC23388.1
PIRiF64139
RefSeqiNP_439885.2, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC23388; AAC23388; HI_1741
GeneIDi950884
KEGGihin:HI1741
PATRICifig|71421.8.peg.1824

Similar proteinsi

Entry informationi

Entry nameiSPOT_HAEIN
AccessioniPrimary (citable) accession number: P43811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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