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Protein

Glutamine synthetase

Gene

glnA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Magnesium 1By similarity1
Metal bindingi136Magnesium 2By similarity1
Binding sitei212ATPBy similarity1
Metal bindingi217Magnesium 2By similarity1
Metal bindingi225Magnesium 2By similarity1
Binding sitei270L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi274Magnesium 1; via pros nitrogenBy similarity1
Binding sitei278ATPBy similarity1
Binding sitei326L-glutamateBy similarity1
Binding sitei332L-glutamateBy similarity1
Binding sitei344ATPBy similarity1
Binding sitei344L-glutamateBy similarity1
Binding sitei349ATPBy similarity1
Binding sitei357ATPBy similarity1
Metal bindingi362Magnesium 1By similarity1
Binding sitei364L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 278ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:HI_0865
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532381 – 472Glutamine synthetaseAdd BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei402O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

STRINGi71421.HI0865.

Structurei

3D structure databases

ProteinModelPortaliP43794.
SMRiP43794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 270L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
KOiK01915.
OMAiIEAAWNT.
PhylomeDBiP43794.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P43794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNANAIANV FKLIEENNVK FVLLRFTDIK GKEHGVSIPV SLVDEDMFED
60 70 80 90 100
GKMFDGSSVE GWKTINKADM LLMPMAETAI VDPFAQIPTL SIRCSVYEPT
110 120 130 140 150
TMQSYDRDPR SIAIRAENYM RSTGIADQAF FGPEPEFFLF DDVRFNVSMN
160 170 180 190 200
KASFSIDDIE AAWNTNKKYE EGNNAYRPLK KGGYCAVAPI DSAHDIRSEM
210 220 230 240 250
CLILEEMGLV IEAHHHEVAT AGQNEIATKF NTLTLKADET QIYKHVVQNV
260 270 280 290 300
ALEHGKTACF MPKPITGDNG SGMHCNMSLS KDGKNIFQGD KYAGLSETAL
310 320 330 340 350
YYIGGIIKHA KALNAFTNPS TNSYKRLVPG YEAPVLLAYS ASNRSASIRI
360 370 380 390 400
PAVTNPKAIR VEARFPDPLA NPYLAFAALL MAGLDGVVNK IHPGDAMDKN
410 420 430 440 450
LYDLPPEELK DIPAVASSLE EALNSLEKDY EFLTQGGVFA KDFIDAFISI
460 470
KRKEVERLNM APHPVEFEMY YA
Length:472
Mass (Da):52,344
Last modified:November 1, 1995 - v1
Checksum:i160DDD0F91B28046
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22524.1.
PIRiI64098.
RefSeqiNP_439025.1. NC_000907.1.
WP_005693212.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22524; AAC22524; HI_0865.
GeneIDi949878.
KEGGihin:HI0865.
PATRICifig|71421.8.peg.906.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1B_HAEIN
AccessioniPrimary (citable) accession number: P43794
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 7, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families