Reviewed, UniProtKB/Swiss-Prot P43793 (DHE4_HAEIN)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdhA
Ordered Locus Names:	HI0189

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	NADP-specific glutamate dehydrogenase		PRO_0000182770
Sites
	Active site	128	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43793-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4698E0CD6C665A34	FASTA	449	48,660
	10 20 30 40 50 60 MSKVASLDAF LTKVAQRDGY QPEFLQAVRE VFTSIWPFLE ANPKYRSEAL LERLVEPERA 70 80 90 100 110 120 FQFRVAWTDD KGQVQVNRAF RVQFNSAIGP FKGGMRFHPS VNLSILKFLG FEQIFKNALT 130 140 150 160 170 180 TLPMGGAKGG SDFDPKGKSD AEVMRFCQAL MAELYRHVGA DTDVPAGDIG VGGREVGYLA 190 200 210 220 230 240 GYMKKLSNQS ACVFTGRGLS FGGSLIRPEA TGYGLIYFAQ AMLAEKGDSF AGKVVSVSGS 250 260 270 280 290 300 GNVAQYAIEK ALSLGAKVVT CSDSSGYVYD PNGFTTEKLA ALFDIKNTKR GRVKDYAEQF 310 320 330 340 350 360 GLQYFEGKRP WEVQVDIALP CATQNELELS DAQRLIKNGV KLVAEGANMP TTIEATEALL 370 380 390 400 410 420 AADVLFGPGK AANAGGVATS GLEMAQSSQR LYWTAEEVDA QLHRIMLDIH ANCKKYGTIE 430 440 GQENINYVVG ANVAGFVKVA DAMLAQGVY

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21858.1.
PIR	A64053.
RefSeq	NP_438358.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	951095.
GenomeReviews	Gene locus HI0189 in contig L42023_GR.
KEGG	hin:HI0189.
NMPDR	fig|71421.1.peg.179.
TIGR	HI0189.
Phylogenomic databases
HOGENOM	P43793.
OMA	P43793. EMAQNAS.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0189-MON.
BRENDA	1.4.1.4. 109.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_HAEIN

Accession

Primary (citable) accession number: P43793

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)