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P43793 (DHE4_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Gene names
Name:gdhA
Ordered Locus Names:HI_0189
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamate biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449NADP-specific glutamate dehydrogenase
PRO_0000182770

Sites

Active site1281Proton donor By similarity
Binding site921Substrate By similarity
Binding site1131Substrate By similarity
Binding site1161Substrate By similarity
Binding site1671Substrate; via carbonyl oxygen By similarity
Binding site2111NADP By similarity
Binding site2421NADP By similarity
Binding site3801Substrate By similarity
Site1681Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P43793 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4698E0CD6C665A34

FASTA44948,660
        10         20         30         40         50         60 
MSKVASLDAF LTKVAQRDGY QPEFLQAVRE VFTSIWPFLE ANPKYRSEAL LERLVEPERA 

        70         80         90        100        110        120 
FQFRVAWTDD KGQVQVNRAF RVQFNSAIGP FKGGMRFHPS VNLSILKFLG FEQIFKNALT 

       130        140        150        160        170        180 
TLPMGGAKGG SDFDPKGKSD AEVMRFCQAL MAELYRHVGA DTDVPAGDIG VGGREVGYLA 

       190        200        210        220        230        240 
GYMKKLSNQS ACVFTGRGLS FGGSLIRPEA TGYGLIYFAQ AMLAEKGDSF AGKVVSVSGS 

       250        260        270        280        290        300 
GNVAQYAIEK ALSLGAKVVT CSDSSGYVYD PNGFTTEKLA ALFDIKNTKR GRVKDYAEQF 

       310        320        330        340        350        360 
GLQYFEGKRP WEVQVDIALP CATQNELELS DAQRLIKNGV KLVAEGANMP TTIEATEALL 

       370        380        390        400        410        420 
AADVLFGPGK AANAGGVATS GLEMAQSSQR LYWTAEEVDA QLHRIMLDIH ANCKKYGTIE 

       430        440 
GQENINYVVG ANVAGFVKVA DAMLAQGVY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC21858.1.
PIRA64053.
RefSeqNP_438358.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43793.
SMRP43793. Positions 6-449.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0189.

Proteomic databases

PRIDEP43793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC21858; AAC21858; HI_0189.
GeneID951095.
KEGGhin:HI0189.
PATRIC20188875. VBIHaeInf48452_0194.

Phylogenomic databases

eggNOGCOG0334.
KOK00262.
OMAIMLDIHA.
OrthoDBEOG65XN4D.
PhylomeDBP43793.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_HAEIN
AccessionPrimary (citable) accession number: P43793
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names